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Surface Micro-Patterned Biofunctionalized Hydrogel for Direct Nucleic Acid Hybridization Detection

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Surface Micro-Patterned Biofunctionalized Hydrogel for Direct Nucleic Acid Hybridization Detection

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dc.contributor.author Zezza, Paola es_ES
dc.contributor.author Lucío, María Isabel es_ES
dc.contributor.author Fernández, Estrella es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.contributor.author Bañuls Polo, María-José es_ES
dc.date.accessioned 2024-01-03T19:03:59Z
dc.date.available 2024-01-03T19:03:59Z
dc.date.issued 2023-03 es_ES
dc.identifier.issn 2079-6374 es_ES
dc.identifier.uri http://hdl.handle.net/10251/201421
dc.description.abstract [EN] The present research is focused on the development of a biofunctionalized hydrogel with a surface diffractive micropattern as a label-free biosensing platform. The biosensors described in this paper were fabricated with a holographic recording of polyethylene terephthalate (PET) surface micro-structures, which were then transferred into a hydrogel material. Acrylamide-based hydrogels were obtained with free radical polymerization, and propargyl acrylate was added as a comonomer, which allowed for covalent immobilization of thiolated oligonucleotide probes into the hydrogel network, via thiol-yne photoclick chemistry. The comonomer was shown to significantly contribute to the immobilization of the probes based on fluorescence imaging. Two different immobilization approaches were demonstrated: during or after hydrogel synthesis. The second approach showed better loading capacity of the bioreceptor groups. Diffraction efficiency measurements of hydrogel gratings at 532 nm showed a selective response reaching a limit of detection in the complementary DNA strand of 2.47 mu M. The label-free biosensor as designed could significantly contribute to direct and accurate analysis in medical diagnosis as it is cheap, easy to fabricate, and works without the need for further reagents. es_ES
dc.description.sponsorship This work was financially supported by the E.U. FEDER, the Spanish Ministry of Science and Innovation (ADBIHOL-PID2019-110713RB-I00/AEI/10.13039/501100011033) and Generalitat Valenciana (PROMETEO/2020/094). M. I. Lucío acknowledges her Juan de la Cierva-Incorporación grant (IJC 2018-035355-I) funded by MCIN/AEI/10.13039/501100011033. P. Zezza acknowledges Generalitat Valenciana for her Grisolia fellowship grant. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Biosensors es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Hydrogels es_ES
dc.subject Surface micropattern es_ES
dc.subject Probe immobilization es_ES
dc.subject Photoclick reaction es_ES
dc.subject Diffraction es_ES
dc.subject Label-free es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Surface Micro-Patterned Biofunctionalized Hydrogel for Direct Nucleic Acid Hybridization Detection es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/bios13030312 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110713RB-I00/ES/HACIA EL BIOSENSADO HOLOGRAFICO AVANZADO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GENERALITAT VALENCIANA//PROMETEO%2F2020%2F094//HOLOGRAFÍA. UNA VIA PARA AFRONTAR NUEVOS RETOS EN BIOSENSADO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//IJC 2018-035355-I/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Zezza, P.; Lucío, MI.; Fernández, E.; Maquieira Catala, A.; Bañuls Polo, M. (2023). Surface Micro-Patterned Biofunctionalized Hydrogel for Direct Nucleic Acid Hybridization Detection. Biosensors. 13(3). https://doi.org/10.3390/bios13030312 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/bios13030312 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.description.issue 3 es_ES
dc.identifier.pmid 36979524 es_ES
dc.identifier.pmcid PMC10046352 es_ES
dc.relation.pasarela S\484104 es_ES
dc.contributor.funder GENERALITAT VALENCIANA es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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