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dc.contributor.author | CARBONELL, ALBERTO![]() |
es_ES |
dc.contributor.author | Martínez de Alba, Ángel-Emilio![]() |
es_ES |
dc.contributor.author | FLORES PEDAUYE, RICARDO![]() |
es_ES |
dc.contributor.author | Gago, Selma![]() |
es_ES |
dc.date.accessioned | 2024-01-24T19:02:21Z | |
dc.date.available | 2024-01-24T19:02:21Z | |
dc.date.issued | 2008-02-05 | es_ES |
dc.identifier.issn | 0042-6822 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/202112 | |
dc.description.abstract | [EN] Infection by viroids, non-protein-coding circular RNAs, occurs with the accumulation of 21¿24 nt viroid-derived small RNAs (vd-sRNAs) with characteristic properties of small interfering RNAs (siRNAs) associated to RNA silencing. The vd-sRNAs most likely derive from dicer-like (DCL) enzymes acting on viroid-specific dsRNA, the key elicitor of RNA silencing, or on the highly structured genomic RNA. Previously, viral dsRNAs delivered mechanically or agroinoculated have been shown to interfere with virus infection in a sequence-specific manner. Here, we report similar results with members of the two families of nuclear- and chloroplast-replicating viroids. Moreover, homologous vd-sRNAs codelivered mechanically also interfered with one of the viroids examined. The interference was sequence-specific, temperature-dependent and, in some cases, also dependent on the dose of the co-inoculated dsRNA or vd-sRNAs. The sequence-specific nature of these effects suggests the involvement of the RNA induced silencing complex (RISC), which provides sequence specificity to RNA silencing machinery. Therefore, viroid titer in natural infections might be regulated by the concerted action of DCL and RISC. Viroids could have evolved their secondary structure as a compromise between resistance to DCL and RISC, which act preferentially against RNAs with compact and relaxed secondary structures, respectively. In addition, compartmentation, association with proteins or active replication might also help viroids to elude their host RNA silencing machinery. | es_ES |
dc.description.sponsorship | This work has been partially supported by grants BFU2005- 06808/BMC from the Ministerio de Educación y Ciencia (MEC) and ACOMP06/141 and ACOMP07/268 from the Generalidad Valenciana (Spain) to R.F. During this work, A.C. received a predoctoral fellowship from the MEC, A.E.M a postdoctoral contract from the MEC, and S.G. a postdoctoral fellowship from the Fundación José y Ana Royo and an I3P contract from the Consejo Superior de Investigaciones Científicas. We thank A. Ahuir for excellent technical assistance and Dr. Lynne Yenush for reviewing the English text. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Virology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | RNA silencing | es_ES |
dc.subject | Dicer | es_ES |
dc.subject | RISC | es_ES |
dc.subject | Pospiviroidae | es_ES |
dc.subject | Avsunviroidae | es_ES |
dc.title | Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.virol.2007.09.031 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//ACOMP06%2F141/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//ACOMP07%2F268/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//BFU2005-06808%2FBMC/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Carbonell, A.; Martínez De Alba, Á.; Flores Pedauye, R.; Gago, S. (2008). Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families. Virology. 371(1):44-53. https://doi.org/10.1016/j.virol.2007.09.031 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.virol.2007.09.031 | es_ES |
dc.description.upvformatpinicio | 44 | es_ES |
dc.description.upvformatpfin | 53 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 371 | es_ES |
dc.description.issue | 1 | es_ES |
dc.identifier.pmid | 18028975 | es_ES |
dc.relation.pasarela | S\31824 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia | es_ES |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas | es_ES |