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dc.contributor.author | CARBONELL, ALBERTO![]() |
es_ES |
dc.contributor.author | La Peña Del Rivero, Marcos De![]() |
es_ES |
dc.contributor.author | FLORES PEDAUYE, RICARDO![]() |
es_ES |
dc.contributor.author | Gago, Selma![]() |
es_ES |
dc.date.accessioned | 2024-01-24T19:02:52Z | |
dc.date.available | 2024-01-24T19:02:52Z | |
dc.date.issued | 2006-11 | es_ES |
dc.identifier.issn | 0305-1048 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/202121 | |
dc.description.abstract | [EN] Eggplant latent viroid (ELVd) can form stable hammerhead structures in its (+) and (¿) strands. These ribozymes have the longest helices I reported in natural hammerheads, with that of the ELVd (+) hammerhead being particularly stable (5/7 bp are G-C). Moreover, the trinucleotide preceding the self-cleavage site of this hammerhead is AUA, which together with GUA also found in some natural hammerheads, deviate from the GUC present in most natural hammerheads including the ELVd (¿) hammerhead. When the AUA trinucleotide preceding the self-cleavage site of the ELVd (+) hammerhead was substituted by GUA and GUC, as well as by AUC (essentially absent in natural hammerheads), the values of the self-cleavage rate constants at low magnesium of the purified hammerheads were: ELVd-(+)-AUC¿ELVd-(+)-GUC>ELVd-(+)-GUA> ELVd-(+)-AUA. However, the ELVd-(+)-AUC hammerhead was the catalytically less efficient during in vitro transcription, most likely because of the transient adoption of catalytically-inactive metastable structures. These results suggest that natural hammerheads have been evolutionary selected to function co-transcriptionally, and provide a model explaining the lack of trinucleotide AUC preceding the self-cleavage site of most natural hammerheads. Comparisons with other natural hammerheads showed that the ELVd-(+)-GUC and ELVd-(+)-AUC hammerheads are the catalytically most active in a post-transcriptional context with low magnesium. | es_ES |
dc.description.sponsorship | This work has been partially supported by grants BFU2005- 06808/BMC from the Ministerio de Educacion y Ciencia (MEC) and ACOMP06/141 from the Generalidad Valenciana (Spain). A.C. has been supported by a pre-doctoral fellowship from the MEC, M.D.P. by a long-term postdoctoral fellowship of the International Human Frontier Science Program Organization and by a Ramon y Cajal contract from the MEC, and S.G. by a postdoctoral fellowship from the Fundacion Jose y Ana Royo and by an I3P contract from the Consejo Superior de Investigaciones Cientificas. Funding to pay the Open Access publication charges for this article was provided by the MEC. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.relation.ispartof | Nucleic Acids Research | es_ES |
dc.rights | Reconocimiento - No comercial (by-nc) | es_ES |
dc.subject | Eggplant latent viroid (ELVd) | es_ES |
dc.subject | Hammerhead ribozyme | es_ES |
dc.subject | Trinucleotide AUC | es_ES |
dc.title | Effects of the trinucleotide preceding the self-cleavage site on eggplant latent viroid hammerheads: differences in co- and post-transcriptional self-cleavage may explain the lack of trinucleotide AUC in most natural hammerheads | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1093/nar/gkl717 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//ACOMP06%2F141/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//BFU2005-06808%2FBMC/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Carbonell, A.; La Peña Del Rivero, MD.; Flores Pedauye, R.; Gago, S. (2006). Effects of the trinucleotide preceding the self-cleavage site on eggplant latent viroid hammerheads: differences in co- and post-transcriptional self-cleavage may explain the lack of trinucleotide AUC in most natural hammerheads. Nucleic Acids Research. 34(19):5613-5622. https://doi.org/10.1093/nar/gkl717 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1093/nar/gkl717 | es_ES |
dc.description.upvformatpinicio | 5613 | es_ES |
dc.description.upvformatpfin | 5622 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 34 | es_ES |
dc.description.issue | 19 | es_ES |
dc.identifier.pmid | 17028097 | es_ES |
dc.identifier.pmcid | PMC1636495 | es_ES |
dc.relation.pasarela | S\29244 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia | es_ES |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas | es_ES |