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Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy

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Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy

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dc.contributor.author Juste-Dolz, Augusto Miguel es_ES
dc.contributor.author Fernández-Sánchez, María Estrella es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Avella-Oliver, Miquel es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.date.accessioned 2024-01-31T19:02:26Z
dc.date.available 2024-01-31T19:02:26Z
dc.date.issued 2023-02 es_ES
dc.identifier.issn 2079-6374 es_ES
dc.identifier.uri http://hdl.handle.net/10251/202267
dc.description.abstract [EN] This paper focuses on creating one-dimensional diffractive grooved structures of antigen proteins on glass substrates for the label-free detection of antibodies to dairy allergens. In particular, the fabrication of protein structures is carried out by combining microcontact printing with physisorption, imines coupling, and thiol-ene click chemistry. The work first sets up these patterning methods and discusses and compares the main aspects involved in them (structure, biolayer thickness, functionality, stability). Homogeneous periodic submicron structures of proteins are created and characterized by diffractive measurements, AFM, FESEM, and fluorescence scanning. Then, this patterning method is applied to proteins involved in cow milk allergy, and the resulting structures are implemented as optical transducers to sense specific immunoglobulins G. In particular, gratings of bovine serum albumin, casein, and ß-lactoglobulin are created and assessed, reaching limits of detection in the range of 30¿45 ng·mL¿1 of unlabeled antibodies by diffractive biosensing. es_ES
dc.description.sponsorship Grant PID2019-110713RB-I00 funded by MCIN/AEI/10.13039/501100011033 and cofunded by "ERDF A way of making Europe", Generalitat Valenciana (PROMETEO/2020/094), and Universitat Politecnica de Valencia (PAID-06-22). es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Biosensors es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Diffraction es_ES
dc.subject Grating es_ES
dc.subject Microcontact printing es_ES
dc.subject Casein es_ES
dc.subject Bovine serum albumin es_ES
dc.subject SS-lactoglobulin es_ES
dc.subject Covalent es_ES
dc.subject Immunoglobulin G es_ES
dc.subject Dairy es_ES
dc.subject Beef es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/bios13020214 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110713RB-I00/ES/HACIA EL BIOSENSADO HOLOGRAFICO AVANZADO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-06-22//Erradicación del riesgo de obstrucción de drenajes por arrastre de mobiliario urbano y vehículos por inundación (ERODAMUVI)/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2020%2F094//HOLOGRAFÍA. UNA VIA PARA AFRONTAR NUEVOS RETOS EN BIOSENSADO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Juste-Dolz, AM.; Fernández-Sánchez, ME.; Puchades, R.; Avella-Oliver, M.; Maquieira Catala, A. (2023). Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy. Biosensors. 13(2). https://doi.org/10.3390/bios13020214 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/bios13020214 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.description.issue 2 es_ES
dc.identifier.pmid 36831980 es_ES
dc.identifier.pmcid PMC9953870 es_ES
dc.relation.pasarela S\482021 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Universitat Politècnica de València es_ES


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