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dc.contributor.author | Juste-Dolz, Augusto Miguel | es_ES |
dc.contributor.author | Fernández-Sánchez, María Estrella | es_ES |
dc.contributor.author | Puchades, Rosa | es_ES |
dc.contributor.author | Avella-Oliver, Miquel | es_ES |
dc.contributor.author | Maquieira Catala, Angel | es_ES |
dc.date.accessioned | 2024-01-31T19:02:26Z | |
dc.date.available | 2024-01-31T19:02:26Z | |
dc.date.issued | 2023-02 | es_ES |
dc.identifier.issn | 2079-6374 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/202267 | |
dc.description.abstract | [EN] This paper focuses on creating one-dimensional diffractive grooved structures of antigen proteins on glass substrates for the label-free detection of antibodies to dairy allergens. In particular, the fabrication of protein structures is carried out by combining microcontact printing with physisorption, imines coupling, and thiol-ene click chemistry. The work first sets up these patterning methods and discusses and compares the main aspects involved in them (structure, biolayer thickness, functionality, stability). Homogeneous periodic submicron structures of proteins are created and characterized by diffractive measurements, AFM, FESEM, and fluorescence scanning. Then, this patterning method is applied to proteins involved in cow milk allergy, and the resulting structures are implemented as optical transducers to sense specific immunoglobulins G. In particular, gratings of bovine serum albumin, casein, and ß-lactoglobulin are created and assessed, reaching limits of detection in the range of 30¿45 ng·mL¿1 of unlabeled antibodies by diffractive biosensing. | es_ES |
dc.description.sponsorship | Grant PID2019-110713RB-I00 funded by MCIN/AEI/10.13039/501100011033 and cofunded by "ERDF A way of making Europe", Generalitat Valenciana (PROMETEO/2020/094), and Universitat Politecnica de Valencia (PAID-06-22). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | Biosensors | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Diffraction | es_ES |
dc.subject | Grating | es_ES |
dc.subject | Microcontact printing | es_ES |
dc.subject | Casein | es_ES |
dc.subject | Bovine serum albumin | es_ES |
dc.subject | SS-lactoglobulin | es_ES |
dc.subject | Covalent | es_ES |
dc.subject | Immunoglobulin G | es_ES |
dc.subject | Dairy | es_ES |
dc.subject | Beef | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/bios13020214 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110713RB-I00/ES/HACIA EL BIOSENSADO HOLOGRAFICO AVANZADO/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPV//PAID-06-22//Erradicación del riesgo de obstrucción de drenajes por arrastre de mobiliario urbano y vehículos por inundación (ERODAMUVI)/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2020%2F094//HOLOGRAFÍA. UNA VIA PARA AFRONTAR NUEVOS RETOS EN BIOSENSADO/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.description.bibliographicCitation | Juste-Dolz, AM.; Fernández-Sánchez, ME.; Puchades, R.; Avella-Oliver, M.; Maquieira Catala, A. (2023). Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy. Biosensors. 13(2). https://doi.org/10.3390/bios13020214 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/bios13020214 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 13 | es_ES |
dc.description.issue | 2 | es_ES |
dc.identifier.pmid | 36831980 | es_ES |
dc.identifier.pmcid | PMC9953870 | es_ES |
dc.relation.pasarela | S\482021 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |