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dc.contributor.author | Almudéver-Folch, Patricia![]() |
es_ES |
dc.contributor.author | Peris, José-Esteban![]() |
es_ES |
dc.contributor.author | Garrigues, Teresa![]() |
es_ES |
dc.contributor.author | Díez-Sales, Octavio![]() |
es_ES |
dc.contributor.author | Melero, Ana![]() |
es_ES |
dc.contributor.author | Alós, Manuel![]() |
es_ES |
dc.date.accessioned | 2024-02-07T19:02:47Z | |
dc.date.available | 2024-02-07T19:02:47Z | |
dc.date.issued | 2010-03-15 | es_ES |
dc.identifier.issn | 1570-0232 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/202407 | |
dc.description.abstract | [EN] A simple, sensitive and specific high-performance liquid chromatography method has been developed for the determination of nortriptyline (NT) in plasma samples. The assay involved derivatization with 9H-fluoren-9-ylmethyl chloroformate (Fmoc-Cl) and isocratic reversed-phase (C-18) chromatography with fluorescence detection. The developed method required only 100 mu l of plasma sample, deproteinized and derivatized in one step. Calibration curves were lineal over the concentration range of 5-5000 ng/ml. The derivatization reaction was performed at room temperature in 20 min and the obtained NT derivative was stable for at least 48 h at room temperature. The within-day and between-day relative standard deviation was below 8%. The limit of detection (LOD) was 2 ng/ml, and the lower limit of quantification (LLOQ) was established at 10 ng/ml. The method was applied on plasma collected from rats, at different time intervals, after intravenous administration of 0.5 mg of NT. | es_ES |
dc.description.sponsorship | This research was supported by Institut de Salud Carlos III grant FIS (FI 060944 953/2006). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Journal of Chromatography B | es_ES |
dc.rights | Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) | es_ES |
dc.subject | Derivatization | es_ES |
dc.subject | Fmoc chloride | es_ES |
dc.subject | Nortriptyline | es_ES |
dc.subject | HPLC | es_ES |
dc.subject.classification | MICROBIOLOGIA | es_ES |
dc.title | Quantification of nortriptyline in plasma by HPLC and fluorescence detection | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.jchromb.2010.01.033 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ISCIII//FIS (FI 060944 953%2F2006)./ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.description.bibliographicCitation | Almudéver-Folch, P.; Peris, J.; Garrigues, T.; Díez-Sales, O.; Melero, A.; Alós, M. (2010). Quantification of nortriptyline in plasma by HPLC and fluorescence detection. Journal of Chromatography B. 879(9-10):841-844. https://doi.org/10.1016/j.jchromb.2010.01.033 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.jchromb.2010.01.033 | es_ES |
dc.description.upvformatpinicio | 841 | es_ES |
dc.description.upvformatpfin | 844 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 879 | es_ES |
dc.description.issue | 9-10 | es_ES |
dc.identifier.pmid | 20172767 | es_ES |
dc.relation.pasarela | S\429748 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.subject.ods | 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades | es_ES |