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dc.contributor.author | Marco-Jiménez, Francisco | es_ES |
dc.contributor.author | Garcia-Dominguez, Ximo | es_ES |
dc.contributor.author | García-Valero, Luis | es_ES |
dc.contributor.author | Vicente Antón, José Salvador | es_ES |
dc.date.accessioned | 2024-04-22T18:06:45Z | |
dc.date.available | 2024-04-22T18:06:45Z | |
dc.date.issued | 2023-03 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/203668 | |
dc.description.abstract | [EN] Although many devices have been developed to reduce sample volume, with an explosion of methods appearing in the literature over the last decade, commercially available devices with simultaneous vitrification of a larger number of embryos are scarce, with the apparent gap for their use in prolific livestock species. In this study, we investigated the effectiveness of a new three-dimensional (3D)-printed device that combines minimum volume cooling vitrification with simultaneous vitrification of a larger number of rabbit embryos. Late morulae/early blastocysts were vitrified with the open Cryoeyelet® device (n = 175; 25 embryos per device), the open Cryotop® device (n = 175; 10 embryos per device), and the traditional closed French mini-straw device (n = 125; 25 embryos per straw) and compared in terms of in vitro development and reproductive performance after transfer to adoptive mothers. Fresh embryos constituted the control group (n = 125). In experiment 1, there was no difference in the development rate to the blastocyst hatching stage between the CryoEyelet® and the other devices. In experiment 2, the CryoEyelet® device showed a higher implantation rate compared with the Cryotop® (6.3% unit of SD, p = 0.87) and French mini-straw® (16.8% unit of SD, p = 1.00) devices. In terms of offspring rate, the CryoEyelet® device was similar to the Cryotop® device but superior to the French straw device. Regarding embryonic and fetal losses, the CryoEyelet® showed lower embryonic losses compared to other vitrification devices. The analysis of bodyweight showed that all devices showed a similar outcomes-a higher birthweight but a lower body weight at puberty than those in the fresh transfer embryos group. In summary, the CryoEyelet® device can be used for the vitrification of many late morulae or early blastocyst stage rabbit embryos per device. Further studies should be performed to evaluate the CryoEyelet® device in other polytocous species for the simultaneous vitrification of a large number of embryos. | es_ES |
dc.description.sponsorship | This research was funded by the Funded by MCIN/AEI/10.13039/501100011033 and by the European Union NextGenerationEU /PRTR (PDC2021-120767-I00). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | Animals | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Cryopreservation | es_ES |
dc.subject | Rabbit | es_ES |
dc.subject | Morulae | es_ES |
dc.subject | Blastocyst | es_ES |
dc.subject.classification | PRODUCCION ANIMAL | es_ES |
dc.title | A 3D-Printed Large Holding Capacity Device for Minimum Volume Cooling Vitrification of Embryos in Prolific Livestock Species | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/ani13050791 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PDC2021-120767-I00/ES/DISPOSITIVO PARA LA VITRIFICACION DE OVULOS Y EMBRIONES EN ESPECIES GANADERAS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.description.bibliographicCitation | Marco-Jiménez, F.; Garcia-Dominguez, X.; García-Valero, L.; Vicente Antón, JS. (2023). A 3D-Printed Large Holding Capacity Device for Minimum Volume Cooling Vitrification of Embryos in Prolific Livestock Species. Animals. 13(5). https://doi.org/10.3390/ani13050791 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/ani13050791 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 13 | es_ES |
dc.description.issue | 5 | es_ES |
dc.identifier.eissn | 2076-2615 | es_ES |
dc.identifier.pmid | 36899646 | es_ES |
dc.identifier.pmcid | PMC10000242 | es_ES |
dc.relation.pasarela | S\496764 | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |