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Early Population Dynamics of "Candidatus Liberibacter asiaticus" in Susceptible and Resistant Genotypes After Inoculation With Infected Diaphorina citri Feeding on Young Shoots

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Early Population Dynamics of "Candidatus Liberibacter asiaticus" in Susceptible and Resistant Genotypes After Inoculation With Infected Diaphorina citri Feeding on Young Shoots

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dc.contributor.author Alves, Mónica N. es_ES
dc.contributor.author Cifuentes-Arenas, Juan C. es_ES
dc.contributor.author Raiol-Junior, Laudecir L. es_ES
dc.contributor.author Ferro, Jesús A. es_ES
dc.contributor.author Peña, Leandro es_ES
dc.date.accessioned 2024-05-07T18:06:04Z
dc.date.available 2024-05-07T18:06:04Z
dc.date.issued 2021-06-09 es_ES
dc.identifier.issn 1664-302X es_ES
dc.identifier.uri http://hdl.handle.net/10251/204036
dc.description.abstract [EN] Huanglongbing is a highly destructive citrus disease associated with "Candidatus Liberibacter asiaticus" (Las), a phloem-limited and non-culturable bacterium, naturally transmitted by the psyllid Diaphorina citri. Although diverse approaches have been used to understand the molecular mechanisms involved in the pathogen-host interaction, such approaches have focused on already infected and/or symptomatic plants, missing early events in the initial days post-inoculation. This study aimed to identify the time course of Las multiplication and whole-plant colonization immediately following inoculation by infected psyllids feeding for 2 days. Thus, the experimental approach was to track Las titers after psyllid inoculation in new shoots (NS) of Citrus × sinensis (susceptible), Murraya paniculata (partially resistant), and Bergera koenigii (fully resistant). Soon after psyllid removal, Las titers dropped until the 10-12th days in all three species. Following this, Las titers increased exponentially only in C. × sinensis and M. paniculata, indicating active bacterial multiplication. In C. × sinensis, Las reached a stationary phase at ~5 log Las cells/g of tissue from the 40th day onward, while in M. paniculata, Las increased at a lower rate of up to ~3 log Las cells/g of tissue between the 40th and 60th days, decreasing gradually thereafter and becoming undetectable from the 160th day onward. In B. koenigii, Las titers decreased from the start and remained undetectable. In C. × sinensis, an average of 2.6 log of Las cells/g of tissue was necessary for Las to move out of 50% of the NS in 23.6 days and to colonize the rest of the plant, causing a successful infection. Conversely, the probability of Las moving out of the NS remained below 50% in M. paniculata and zero in B. koenigii. To our knowledge, this is the first study on Las dynamics and whole-plant colonization during the earliest stages of infection. Identification of critical time-points for either successful multiplication or Las resistance may help to elucidate initial events of Las-host interactions that may be missed due to longer sampling intervals and at later stages of infection. es_ES
dc.description.sponsorship This study was financed in part by Fundecitrus, grant no. 817526 from the European Union H2020 Innovation Action Program and Project PID2019-104569RB-I00 from the AEI-Spain, the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior Brazil (CAPES) -Finance Code 001. MA is recipient of a Ph.D. fellowship from CAPES. JF is recipient of a fellowship grant (#312089/2019-8) from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico -Brazil (CNPq). es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Microbiology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Bergera koenigii es_ES
dc.subject Citrus spp. es_ES
dc.subject HLB es_ES
dc.subject Las multiplication es_ES
dc.subject Murraya paniculata es_ES
dc.subject Bacterial growth es_ES
dc.subject QPCR es_ES
dc.title Early Population Dynamics of "Candidatus Liberibacter asiaticus" in Susceptible and Resistant Genotypes After Inoculation With Infected Diaphorina citri Feeding on Young Shoots es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fmicb.2021.683923 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104569RB-I00/ES/MEJORA GENETICA DE PORTAINJERTOS CITRICOS PARA GENERAR RESISTENCIA A VIEJAS Y NUEVAS ENFERMEDADES EMERGENTES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/817526/EU/PREVENTING HLB EPIDEMICS FOR ENSURING CITRUS SURVIVAL IN EUROPE/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CNPq//312089%2F2019-8/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CAPES//001/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Alves, MN.; Cifuentes-Arenas, JC.; Raiol-Junior, LL.; Ferro, JA.; Peña, L. (2021). Early Population Dynamics of "Candidatus Liberibacter asiaticus" in Susceptible and Resistant Genotypes After Inoculation With Infected Diaphorina citri Feeding on Young Shoots. Frontiers in Microbiology. 12. https://doi.org/10.3389/fmicb.2021.683923 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fmicb.2021.683923 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.identifier.pmid 34177870 es_ES
dc.identifier.pmcid PMC8219961 es_ES
dc.relation.pasarela S\459931 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior, Brasil es_ES
dc.contributor.funder Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil es_ES


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