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The Glycolitic Versatility of Bacteroides Uniformis CECT 7771 and and Its Genome Response to Oligo and Polysaccharides.

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The Glycolitic Versatility of Bacteroides Uniformis CECT 7771 and and Its Genome Response to Oligo and Polysaccharides.

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dc.contributor.author Benítez-Páez, Alfonso es_ES
dc.contributor.author Gómez Del Pulgar, Eva M. es_ES
dc.contributor.author Sanz, Yolanda es_ES
dc.date.accessioned 2024-05-08T18:04:10Z
dc.date.available 2024-05-08T18:04:10Z
dc.date.issued 2027-08-25 es_ES
dc.identifier.uri http://hdl.handle.net/10251/204050
dc.description.abstract [EN] Bacteroides spp. are dominant components of the phylum Bacteroidetes in the gut microbiota and prosper in glycan enriched environments. However, knowledge of the machinery of specific species isolated from humans (like Bacteroides uniformis) contributing to the utilization of dietary and endogenous sources of glycans and their byproducts is limited. We have used the cutting-edge nanopore-based technology to sequence the genome of B. uniformis CECT 7771, a human symbiont with a proven pre-clinical efficacy on metabolic and immune dysfunctions in obesity animal models. We have also used massive sequencing approaches to distinguish the genome expression patterns in response to carbon sources of different complexity during growth. At genome-wide level, our analyses globally demonstrate that B. uniformis strains exhibit an expanded glycolytic capability when compared with other Bacteroides species. Moreover, by studying the growth and whole-genome expression of B. uniformis CECT 7771 in response to different carbon sources, we detected a differential growth fitness and expression patterns across the genome depending on the carbon source of the culture media. The dietary fibers used exerted different effects on B. uniformis CECT 7771 activating different molecular pathways and, therefore, allowing the production of different metabolite types with potential impact on gut health. The genome and transcriptome analysis of B. uniformis CECT 7771, in response to different carbon sources, shows its high versatility to utilize both dietary and endogenous glycans along with the production of potentially beneficial end products for both the bacterium and the host, pointing to a mechanistic basis of a mutualistic relationship. es_ES
dc.description.sponsorship This study was supported by grants RTC-2016-5396-1 from the Spanish Ministry of Economy and Competitiveness (MINECO, Spain). The contract to ABP is supported by the EU Project MyNewGut (No. 613979) from the 7th Framework Program. Authors also thank to Joan Vermeiren and to Cargill R&D Centre Europe for the kind donation of WBE fiber for experiments. es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Cellular and Infection Microbiology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Bacteroides uniformis es_ES
dc.subject Polysaccharides es_ES
dc.subject Genome es_ES
dc.subject Transcriptome es_ES
dc.subject Mucin-degrader es_ES
dc.subject GABA es_ES
dc.subject Butyrate es_ES
dc.title The Glycolitic Versatility of Bacteroides Uniformis CECT 7771 and and Its Genome Response to Oligo and Polysaccharides. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fcimb.2017.00383 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/613979/EU/Microbiome Influence on Energy balance and Brain Development-Function Put into Action to Tackle Diet-related Diseases and Behavior./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RTC-2016-5396-1/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Benítez-Páez, A.; Gómez Del Pulgar, EM.; Sanz, Y. (2027). The Glycolitic Versatility of Bacteroides Uniformis CECT 7771 and and Its Genome Response to Oligo and Polysaccharides. Frontiers in Cellular and Infection Microbiology. 7. https://doi.org/10.3389/fcimb.2017.00383 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fcimb.2017.00383 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.identifier.eissn 2235-2988 es_ES
dc.identifier.pmid 28971068 es_ES
dc.identifier.pmcid PMC5609589 es_ES
dc.relation.pasarela S\361728 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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