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The capsid protein of citrus leprosis virus C shows a nuclear distribution and interacts with the nucleolar fibrillarin protein

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The capsid protein of citrus leprosis virus C shows a nuclear distribution and interacts with the nucleolar fibrillarin protein

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dc.contributor.author Leastro, Mikhail Oliveira es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author SANCHEZ NAVARRO, JESUS ANGEL es_ES
dc.date.accessioned 2024-05-16T18:08:25Z
dc.date.available 2024-05-16T18:08:25Z
dc.date.issued 2024-02 es_ES
dc.identifier.issn 0168-1702 es_ES
dc.identifier.uri http://hdl.handle.net/10251/204204
dc.description.abstract [EN] Brevipalpus-transmitted viruses (BTVs) have a significant negative economic impact on the citrus industry in Central and South America. Until now, only a few studies have explored the intracellular distribution and interaction of BTVs-encoded proteins with host factors, particularly for cileviruses, the main BTV responsible for the Citrus Leprosis (CL) disease. This study describes the nuclear localization of citrus leprosis virus C (CiLV-C) capsid protein (p29) and its interaction with the fibrillarin (Fib2) within the nucleolar compartment and cell cytoplasm. Our results, obtained by computer predictions and laser scanning confocal microscopy analyses, including colocalization and bimolecular fluorescence complementation (BiFC) approaches, revealed that a fraction of the p29 is localized in the nucleus and colocalizes with the Fib2 in both the nucleolus and cytosol. The nuclear localization of p29 correlated with a smaller nucleus size. Furthermore, co-immunoprecipitation (Co-IP) assays confirmed the interactions between p29 and Fib2. The implications of these findings for the functionalities of the cilevirus capsid protein are discussed. es_ES
dc.description.sponsorship This work was supported by grant PID2020-115571RB-100 from the Spanish MCIN/AEI/10.13039/501100011033 granting agency and from Prometeo project CIPROM/2022/31 funded by Generalitat Valenciana. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Virus Research es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Cilevirus es_ES
dc.subject CiLV-C es_ES
dc.subject Capsid protein es_ES
dc.subject P29 es_ES
dc.subject Fibrillarin es_ES
dc.subject Nucleus es_ES
dc.title The capsid protein of citrus leprosis virus C shows a nuclear distribution and interacts with the nucleolar fibrillarin protein es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.virusres.2023.199297 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115571RB-I00/ES/LA MODIFICACION N6-METILADENOSINA (M6A) DEL RNA Y EL DIRECCIONAMIENTO DUAL A CLOROPLASTOS Y MITOCONDRIAS COMO MECANISMOS REGULADORES EN LA BIOLOGIA DE LOS VIRUS RNA DE PLANTAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//CIPROM%2F2022%2F31/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Leastro, MO.; Pallás Benet, V.; Sanchez Navarro, JA. (2024). The capsid protein of citrus leprosis virus C shows a nuclear distribution and interacts with the nucleolar fibrillarin protein. Virus Research. 340. https://doi.org/10.1016/j.virusres.2023.199297 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.virusres.2023.199297 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 340 es_ES
dc.identifier.pmid 38070688 es_ES
dc.identifier.pmcid PMC10758971 es_ES
dc.relation.pasarela S\513755 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES


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