Sánchez-Martín, MDM.; Giraldo-Reboloso, E.; Gisbert-Roca, F.; Alastrue-Agudo, A.; Martínez-Ramos, C.; Monleón Pradas, M.; Moreno-Manzano, V. (2023). Acute transplantation of NPC on electrospun poly-lactic acid membranes containing curcumin into the injured spinal cord reduces neuronal degeneration. Frontiers in Biomaterials Science. 2. https://doi.org/10.3389/fbiom.2023.1298894
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/204431
Title:
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Acute transplantation of NPC on electrospun poly-lactic acid membranes containing curcumin into the injured spinal cord reduces neuronal degeneration
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Author:
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Sánchez-Martín, María del Mar
Giraldo-Reboloso, Esther
Gisbert-Roca, Fernando
Alastrue-Agudo, Ana
Martínez-Ramos, Cristina
Monleón Pradas, Manuel
Moreno-Manzano, Victoria
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UPV Unit:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials
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Issued date:
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Abstract:
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[EN] Effective spinal cord injury (SCI) treatment remains a significant challenge, given the complex nature of the primary injury and associated devastating loss of neural activity. Neural progenitor cell (NPC)-based therapy ...[+]
[EN] Effective spinal cord injury (SCI) treatment remains a significant challenge, given the complex nature of the primary injury and associated devastating loss of neural activity. Neural progenitor cell (NPC)-based therapy has emerged as a potent strategy for the treatment of SCI. However, the invasive nature of direct cell transplantation and the need to enhance graft integration into host tissue remain critical issues. We implemented an improved combinatorial approach to SCI treatment by functionalizing electrospun poly-lactic acid (PLA) membranes that support the sustained delivery of curcumin (PLA-curcumin) and act as a carrier for NPC for local transplantation. In vitro experiments demonstrate that curcumin prevents harmful oxidative and inflammatory stress by preventing death and inhibiting NF-¿B activation (mimicked by treatment with hydrogen peroxide or lipopolysaccharide acid). Curcumin also enhances neurite-like outgrowth in NPC and cortical neurons in culture, which may enhance neural connectivity. In vivo transplantation of NPC on a PLA-curcumin electrospun membrane enables cell migration, reduces injured area size, and increases neuronal fiber preservation to induce a slowing of acute neural damage.
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Subjects:
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Poly-lactic acid
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Electrospun membranes
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Curcumin
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NPC
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Spinal cord injury
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Copyrigths:
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Reconocimiento (by)
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Source:
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Frontiers in Biomaterials Science. (eissn:
2813-3749
)
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DOI:
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10.3389/fbiom.2023.1298894
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Publisher:
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Frontiers Media SA
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Publisher version:
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https://doi.org/10.3389/fbiom.2023.1298894
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Project ID:
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-124359OB-I00/ES/ESTRATEGIAS DE TERAPIA CELULAR PARA LA REGENERACION DE LESIONES MEDULARES/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126612OB-I00/ES/CONSTRUCCION EX VIVO DE ESTRUCTURAS PSEUDO-NERVIOSAS Y SU TRASLACION IN VIVO/
info:eu-repo/grantAgreement/EC/H2020/964562/EU/Regeneration of Injured Spinal cord by Electro pUlsed bio-hybrid imPlant/
info:eu-repo/grantAgreement/UPV-VIN//PAID-10-22//El papel de los estímulos mecánicos, eléctricos, magnéticos y biológicos en la regeneración del sistema nervioso
/
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Thanks:
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The authors declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by FEDER/Ministerio de Ciencia e Innovación Agencia Estatal de Investigación ...[+]
The authors declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by FEDER/Ministerio de Ciencia e Innovación Agencia Estatal de Investigación (AEI) PID2021-1243590B-I100 (to VM-M) and PID2021-126612OB-I00 (to MM) funded by MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe, as well as the RISEUP EU grant (Ref. 964562) from FetOpen H2020 program. FG and MM-P acknowledge financing from Vicerrectorado de Investigación de la Universitat Politècnica de València (PAID-10-22). MS-M was supported by a grant from the AEI in the FPI program. Part of the equipment employed in this work was funded by Generalitat Valenciana and co-financed with ERDF funds (OP ERDF of Comunitat Valenciana 2014 2020) and the UE; Fondo Europeo de Desarrollo Regional (FEDER) incluido en el Programa Operativo FEDER de la Comunidad Valenciana 2014-2020.
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Type:
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Artículo
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