- -

A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis

RiuNet: Repositorio Institucional de la Universidad Politécnica de Valencia

Compartir/Enviar a

Citas

Estadísticas

  • Estadisticas de Uso

A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis

Mostrar el registro sencillo del ítem

Ficheros en el ítem

dc.contributor.author Piovesana, Maiara es_ES
dc.contributor.author Wood, Ana K. M. es_ES
dc.contributor.author Smith, Daniel P. es_ES
dc.contributor.author Deery, Michael J. es_ES
dc.contributor.author Bayliss, Richard es_ES
dc.contributor.author Carrera, Esther es_ES
dc.contributor.author Wellner, N es_ES
dc.contributor.author Kosik, Ondrej es_ES
dc.contributor.author Napier, Johnathan A. es_ES
dc.contributor.author Kurup, Smita es_ES
dc.contributor.author Matthes, Michaela C. es_ES
dc.date.accessioned 2024-06-20T18:16:59Z
dc.date.available 2024-06-20T18:16:59Z
dc.date.issued 2023-05-19 es_ES
dc.identifier.issn 0022-0957 es_ES
dc.identifier.uri http://hdl.handle.net/10251/205328
dc.description.abstract [EN] Cysteine-rich receptor-like kinases (CRKs) are a large family of plasma membrane-bound receptors ubiquitous in higher plants. However, despite their prominence, their biological roles have remained largely elusive so far. In this study we report the characterization of an Arabidopsis mutant named crk10-A397T in which alanine 397 has been replaced by a threonine in the aC helix of the kinase domain of CRK10, known to be a crucial regulatory module in mammalian kinases. The crk10-A397T mutant is a dwarf that displays collapsed xylem vessels in the root and hypocotyl, whereas the vasculature of the inflorescence develops normally. In situ phosphorylation assays with His-tagged wild type and crk10-A397T versions of the CRK10 kinase domain revealed that both alleles are active kinases capable of autophosphorylation, with the newly introduced threonine acting as an additional phosphorylation site in crk10-A397T. Transcriptomic analysis of wild type and crk10-A397T mutant hypocotyls revealed that biotic and abiotic stress-responsive genes are constitutively up-regulated in the mutant, and a root-infection assay with the vascular pathogen Fusarium oxysporum demonstrated that the mutant has enhanced resistance to this pathogen compared with wild type plants. Taken together our results suggest that crk10-A397T is a gain-of-function allele of CRK10, the first such mutant to have been identified for a CRK in Arabidopsis. es_ES
dc.description.sponsorship This work was funded by the UK Biotechnology and Biological Sciences Research Council grants BBS/E/C/000I0420 and BB/P016855/1. es_ES
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation.ispartof Journal of Experimental Botany es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Alpha C helix es_ES
dc.subject Arabidopsis es_ES
dc.subject Cysteine-rich receptor-like kinase CRK10 es_ES
dc.subject Defence response es_ES
dc.subject Fusarium oxysporum es_ES
dc.subject Gain-of-function mutation es_ES
dc.subject Kinase regulation es_ES
dc.subject Transcriptomics es_ES
dc.subject Xylem vessel collapse es_ES
dc.title A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/jxb/erad080 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/DEFRA//101948%2F198285%2F6/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/BBSRC//BB%2FP016855%2F1/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/BBSRC//BBS%2FE%2FC%2F000I0420/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Piovesana, M.; Wood, AKM.; Smith, DP.; Deery, MJ.; Bayliss, R.; Carrera, E.; Wellner, N.... (2023). A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis. Journal of Experimental Botany. 74(10):3104-3121. https://doi.org/10.1093/jxb/erad080 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1093/jxb/erad080 es_ES
dc.description.upvformatpinicio 3104 es_ES
dc.description.upvformatpfin 3121 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 74 es_ES
dc.description.issue 10 es_ES
dc.identifier.pmid 36869735 es_ES
dc.identifier.pmcid PMC10199123 es_ES
dc.relation.pasarela S\513631 es_ES
dc.contributor.funder Department for Environment, Food and Rural Affairs, UK Government es_ES
dc.contributor.funder Biotechnology and Biological Sciences Research Council, Reino Unido es_ES


Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem