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dc.contributor.author | Calabuig-Serna, Antonio | es_ES |
dc.contributor.author | Mir Moreno, Ricardo | es_ES |
dc.contributor.author | Seguí-Simarro, Jose M. | es_ES |
dc.date.accessioned | 2024-07-02T18:08:59Z | |
dc.date.available | 2024-07-02T18:08:59Z | |
dc.date.issued | 2023-03 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/205726 | |
dc.description.abstract | [EN] In this work, we studied the induction of somatic embryogenesis in Arabidopsis using IZEs as explants. We characterized the process at the light and scanning electron microscope level and studied several specific aspects such as WUS expression, callose deposition, and principally Ca2+ dynamics during the first stages of the process of embryogenesis induction, by confocal FRET analysis with an Arabidopsis line expressing a cameleon calcium sensor. We also performed a pharmacological study with a series of chemicals know to alter calcium homeostasis (CaCl2, inositol 1,4,5-trisphosphate, ionophore A23187, EGTA), the calcium-calmodulin interaction (chlorpromazine, W-7), and callose deposition (2-deoxy-D-glucose). We showed that, after determination of the cotiledonary protrusions as embryogenic regions, a finger-like appendix may emerge from the shoot apical region and somatic embryos are produced from the WUS-expressing cells of the appendix tip. Ca2+ levels increase and callose is deposited in the cells of the regions where somatic embryos will be formed, thereby constituting early markers of the embryogenic regions. We also found that Ca2+ homeostasis in this system is strictly maintained and cannot be altered to modulate embryo production, as shown for other systems. Together, these results contribute to a better knowledge and understanding of the process of induction of somatic embryos in this system. | es_ES |
dc.description.sponsorship | This research was funded by MCIN/AEI/10.13039/501100011033, grant number PID2020- 115763RB-I00 to JMSS and by Generalitat Valenciana, grant number CDEIGENT 2018/023 to RM. ACS is recipient of a predoctoral contract from the FPU program of the Spanish Government. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation.ispartof | Plants | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | 2-deoxy-D-glucose | es_ES |
dc.subject | Chlorpromazine | es_ES |
dc.subject | EGTA | es_ES |
dc.subject | FRET | es_ES |
dc.subject | In vitro culture | es_ES |
dc.subject | Inositol 1,4,5-trisphosphate | es_ES |
dc.subject | Ionophore A23187 | es_ES |
dc.subject | W-7 | es_ES |
dc.subject.classification | GENETICA | es_ES |
dc.title | Calcium dynamics, WUSCHEL expression and callose deposition during somatic embryogenesis in Arabidopsis thaliana immature zygotic embryos | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/plants12051021 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115763RB-I00/ES/ESTUDIO DEL PAPEL DEL CALCIO EN LA INDUCCION DE EMBRIOGENESIS IN VITRO EN ESPECIES VEGETALES MODELO Y RECALCITRANTES/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//CIDEGENT%2F2018%2F023/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.description.bibliographicCitation | Calabuig-Serna, A.; Mir Moreno, R.; Seguí-Simarro, JM. (2023). Calcium dynamics, WUSCHEL expression and callose deposition during somatic embryogenesis in Arabidopsis thaliana immature zygotic embryos. Plants. 12(5). https://doi.org/10.3390/plants12051021 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/plants12051021 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 12 | es_ES |
dc.description.issue | 5 | es_ES |
dc.identifier.eissn | 2223-7747 | es_ES |
dc.identifier.pmid | 36903882 | es_ES |
dc.identifier.pmcid | PMC10005541 | es_ES |
dc.relation.pasarela | S\483778 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | AGENCIA ESTATAL DE INVESTIGACION | es_ES |
upv.costeAPC | 1500 | es_ES |