Blanes-García, M.; Marinovic, Z.; Herranz-Jusdado, JG.; Xie, X.; Ferrao, L.; Gallego Albiach, V.; Pérez Igualada, LM.... (2024). Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla). Fish Physiology and Biochemistry. https://doi.org/10.1007/s10695-024-01338-1
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/206520
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Título:
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Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla)
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Autor:
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Blanes-García, Marta
Marinovic, Zoran
Herranz-Jusdado, Juan Germán
Xie, Xuan
Ferrao, Leonor
Gallego Albiach, Victor
Pérez Igualada, Luz María
Horváth, Ákos
Psenicka, Martin
Asturiano, Juan F.
Morini, Marina
Baloch, Abdul Rasheed
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Entidad UPV:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
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Fecha difusión:
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Resumen:
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[EN] Identification of specific molecular markers for spermatogonial stem cells in teleost is crucial for enhancing the efficacy of reproductive biotechnologies in aquaculture, such as transplantation and surrogate production ...[+]
[EN] Identification of specific molecular markers for spermatogonial stem cells in teleost is crucial for enhancing the efficacy of reproductive biotechnologies in aquaculture, such as transplantation and surrogate production in fishes. Since it is not yet possible to distinguish spermatogonial stem cells of European eel (Anguilla anguilla) using specific molecular markers, we isolated spermatogonial cells from immature European eels to find these potential markers. We attempted this by studying three candidate genes: vasa, nanos2, and dnd1. Two vasa (vasa1 and vasa2) genes, nanos2, and dnd1 were identified, characterized, and studied in the muscle, testis, and isolated spermatogonia. Our results showed that vasa1 and vasa2 had the highest levels of expression when measured by qPCR. In situ hybridization and immunochemistry assays showed that the four genes were localized explicitly in type A spermatogonia. However, vasa1 and vasa2 exhibited stronger signals in the immature testicular tissue than the other two potential markers. According to this, vasa1 and vasa2 were found to be the most effective markers for spermatogonial cells in the European eel.
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Palabras clave:
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Testis
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Fish
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Gene expression
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Vasa
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Dnd1
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Nanos2
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Derechos de uso:
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Reconocimiento (by)
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Fuente:
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Fish Physiology and Biochemistry. (issn:
0920-1742
)
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DOI:
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10.1007/s10695-024-01338-1
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Editorial:
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Springer-Verlag
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Versión del editor:
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https://doi.org/10.1007/s10695-024-01338-1
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Código del Proyecto:
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096413-B-I00/ES/DESARROLLO Y APLICACION DE LAS TECNICAS DE PRODUCCION SUBROGADA DE GAMETOS DE ANGUILA EUROPEA MEDIANTE XENOTRASPLANTE DE ESPERMATOGONIAS EN OTRAS ESPECIES DE PECES/
...[+]
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096413-B-I00/ES/DESARROLLO Y APLICACION DE LAS TECNICAS DE PRODUCCION SUBROGADA DE GAMETOS DE ANGUILA EUROPEA MEDIANTE XENOTRASPLANTE DE ESPERMATOGONIAS EN OTRAS ESPECIES DE PECES/
info:eu-repo/grantAgreement/EC/H2020/642893/EU/Improved production strategies for endangered freshwater species./
info:eu-repo/grantAgreement/UPV//PAID-01-20/
info:eu-repo/grantAgreement/GVA//GRISOLIAP%2F2020%2F063/
info:eu-repo/grantAgreement/GVA//THINKINAZUL%2F2021%2F012/
info:eu-repo/grantAgreement/MEYS//LM2018099/
info:eu-repo/grantAgreement/GACR//22-31141 J/
info:eu-repo/grantAgreement/MICINN//RYC2021-031558-I/
info:eu-repo/grantAgreement/MICINN//PRTR-C17.I1/
info:eu-repo/grantAgreement/ITM//TKP2020- NKA-16/
info:eu-repo/grantAgreement/ITM//NKFIH K138425/
info:eu-repo/grantAgreement/ITM//FK 142933/
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Agradecimientos:
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Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Funded by the European Union s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement Nº 642893 ...[+]
Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Funded by the European Union s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement Nº 642893 (IMPRESS; including the JGHJ and ARB predoctoral contracts), and by the Ministerio de Ciencia e Innovación y Universidades (Project EELGONIA; RTI2018-096413-BI00). MBG and LF have PhD contracts from the UPV (PAID01 20) and GVA (GRISOLIAP/2020/063), respectively. VG joined a grant RYC2021-031558-I funded by MCIN/AEI/10.13039/501100011033, and by European Union NextGenerationEU/PRTR. MM has a postdoc contract supported byMCIN/AEI/10.13039/501100011033 funded by European Union NextGenerationEU/PRTR (C17.I1) and by Generalitat Valenciana (THINKINAZUL/2021/012) to SEASPERM. MP was fnancially supported by the Ministry of Education, Youth and Sports
of the Czech Republic project CENAKVA (LM2018099) and Biodiversity (CZ.02.1.01/0.0/0.0/16_025/0007370), and the
Czech Science Foundation (grant number 22-31141 J)). ZM and ÁH were supported by the Ministry of Innovation and Technology within the framework of the Thematic Excellence Programme 2020, National Challenges Subprogramme (TKP2020-NKA-16) as well as the NKFIH K138425 and FK 142933 projects. Funding for open access charge: CRUE-Universitat Politècnica de València.
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Tipo:
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Artículo
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