Palkina, KA.; Karataeva, TA.; Perfilov, MM.; Fakhranurova, LI.; Markina, NM.; Gonzalez Somermeyer, L.; Garcia-Perez, E.... (2024). A hybrid pathway for self-sustained luminescence. Science Advances. 10(10). https://doi.org/10.1126/sciadv.adk1992
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/207831
Título:
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A hybrid pathway for self-sustained luminescence
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Autor:
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Palkina, Kseniia A.
Karataeva, Tatiana A.
Perfilov, Maxim M.
Fakhranurova, Liliia I.
Markina, Nadezhda M.
Gonzalez Somermeyer, Louisa
Garcia-Perez, Elena
Vázquez-Vilar, Marta
Rodriguez-Rodriguez, Marta
Vazquez-Vilriales, Victor
Shakhova, Ekaterina S.
Mitiouchkina, Tatiana
Belozerova, Olga A.
Kovalchuk, Sergey I.
Alekberova, Anna
Orzáez Calatayud, Diego Vicente
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Entidad UPV:
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Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
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Fecha difusión:
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Resumen:
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[EN] The fungal bioluminescence pathway can be reconstituted in other organisms allowing luminescence imaging without exogenously supplied substrate. The pathway starts from hispidin biosynthesis-a step catalyzed by a large ...[+]
[EN] The fungal bioluminescence pathway can be reconstituted in other organisms allowing luminescence imaging without exogenously supplied substrate. The pathway starts from hispidin biosynthesis-a step catalyzed by a large fungal polyketide synthase that requires a posttranslational modification for activity. Here, we report identification of alternative compact hispidin synthases encoded by a phylogenetically diverse group of plants. A hybrid bioluminescence pathway that combines plant and fungal genes is more compact, not dependent on availability of machinery for posttranslational modifications, and confers autonomous bioluminescence in yeast, mammalian, and plant hosts. The compact size of plant hispidin synthases enables additional modes of delivery of autoluminescence, such as delivery with viral vectors.
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Palabras clave:
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Cell-Suspension Cultures
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III Polyketide Synthases
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Chalcone Synthase
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Expression System
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Biosynthesis
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Protein
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Derechos de uso:
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Reconocimiento (by)
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Fuente:
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Science Advances. (eissn:
2375-2548
)
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DOI:
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10.1126/sciadv.adk1992
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Editorial:
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American Association for the Advancement of Science (AAAS)
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Versión del editor:
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https://doi.org/10.1126/sciadv.adk1992
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Código del Proyecto:
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108203RB-I00/ES/EXPLOITING THE MODULAR ARCHITECTURE OF CRISPR%2FCAS TO DESIGN PROGRAMMABLE GENE CIRCUITS AND OTHER NEW BREEDING TOOLS IN PLANTS/
info:eu-repo/grantAgreement/RFBR//21-54-52004/
info:eu-repo/grantAgreement/RSF//22-14-00400/
info:eu-repo/grantAgreement/RSF//22-74-00124/
info:eu-repo/grantAgreement/UKRI//UKRI MC-A658-5QEA0/
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Agradecimientos:
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This study was partially funded by light Bio and Planta. the Synthetic biology Group is funded by the MRC London Institute of Medical Sciences (UKRi Mc-A658- 5QeA0). Cloning and luminescent assays performed in BY-2 were ...[+]
This study was partially funded by light Bio and Planta. the Synthetic biology Group is funded by the MRC London Institute of Medical Sciences (UKRi Mc-A658- 5QeA0). Cloning and luminescent assays performed in BY-2 were partially supported by RSF, project number 22-14-00400, https://rscf.ru/project/22-14-00400/. Plant transformations were funded by RFBR and MOST, project number 21-54-52004. Plant imaging experiments were funded by RSF, project number 22-74-00124, https://rscf.ru/project/22-74-00124/. viral delivery experiments were funded by the grant PID2019-108203RB-I00 Plan nacional I+D from the Ministerio de ciencia e innovacion (Spain) through the Agencia estatal de investigacion (cofinanced by the European Regional Development Fund)
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Tipo:
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Artículo
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