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ß-Galactosidase-Triggered Photodynamic Elimination of Senescent Cells with a Boron Dipyrromethene-Based Photosensitizer

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ß-Galactosidase-Triggered Photodynamic Elimination of Senescent Cells with a Boron Dipyrromethene-Based Photosensitizer

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dc.contributor.author Chu, Jacky C. H. es_ES
dc.contributor.author Escriche-Navarro, Blanca es_ES
dc.contributor.author Xiong, Junlong es_ES
dc.contributor.author García-Fernández, Alba es_ES
dc.contributor.author Martínez-Máñez, Ramón es_ES
dc.contributor.author Ng, Dennis K. P. es_ES
dc.date.accessioned 2024-10-03T18:26:37Z
dc.date.available 2024-10-03T18:26:37Z
dc.date.issued 2024-06 es_ES
dc.identifier.uri http://hdl.handle.net/10251/209279
dc.description.abstract [EN] Senescence is a cellular response having physiological and reparative functions to preserve tissue homeostasis and suppress tumor growth. However, the accumulation of senescent cells would cause deleterious effects that lead to age-related dysfunctions and cancer progression. Hence, selective detection and elimination of senescent cells are crucial yet remain a challenge. A beta-galactosidase (beta-gal)-activated boron dipyrromethene (BODIPY)-based photosensitizer (compound 1) is reported here that can selectively detect and eradicate senescent cells. It contains a galactose moiety connected to a pyridinium BODIPY via a self-immolative nitrophenylene linker, of which the photoactivity is effectively quenched. Upon interactions with the senescence-associated beta-gal, it undergoes enzymatic hydrolysis followed by self-immolation, leading to the release of an activated BODIPY moiety by which the fluorescence emission and singlet oxygen generation are restored. The ability of 1 to detect and eliminate senescent cells is demonstrated in vitro and in vivo, using SK-Mel-103 tumor-bearing mice treated with senescence-inducing therapy. The results demonstrate that 1 can be selectively activated in senescent cells to trigger a robust senolytic effect upon irradiation. This study breaks new ground in the design and application of new senolytic agents based on photodynamic therapy.; A tailor-made photosensitizer based on boron dipyrromethene conjugated with a galactose moiety through a self-immolative linker has been designed and synthesized. It exhibits a selective response toward the senescence-associated beta-galactosidase, rendering it an efficient theranostic behavior for detection and photodynamic elimination of senescent cells in vitro and in vivo. image es_ES
dc.description.sponsorship J.C.H.C., B.E.N., and J.X. contributed equally to this work. This work was supported by a General Research Fund from the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. 14307518). The authors also acknowledge the financial support from Project PID2021-126304OB-C41 funded by MCIN/AEI/10.13039/501100011033/ and by European Regional Development Fund - A way of doing Europe and Project CIPROM/2021/007 funded by Generalitat Valenciana. Parts of the Table of Contents graphic and Figure 1A were created with BioRender.com. es_ES
dc.language Inglés es_ES
dc.publisher Wiley-VCH Verlag GmbH & Co. KGaA es_ES
dc.relation.ispartof Advanced Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Boron dipyrromethene es_ES
dc.subject Beta-galactosidase es_ES
dc.subject Photodynamic therapy es_ES
dc.subject Senescent cells es_ES
dc.subject Singlet oxygen es_ES
dc.subject.classification QUIMICA INORGANICA es_ES
dc.title ß-Galactosidase-Triggered Photodynamic Elimination of Senescent Cells with a Boron Dipyrromethene-Based Photosensitizer es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/advs.202401012 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126304OB-C41/ES/NUEVOS MATERIALES Y SONDAS PARA EL RECONOCIMIENTO, LIBERACION DE FARMACOS, NANOMOTORES Y COMUNICACION/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//CIPROM%2F2021%2F007/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/RGC//14307518/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Chu, JCH.; Escriche-Navarro, B.; Xiong, J.; García-Fernández, A.; Martínez-Máñez, R.; Ng, DKP. (2024). ß-Galactosidase-Triggered Photodynamic Elimination of Senescent Cells with a Boron Dipyrromethene-Based Photosensitizer. Advanced Science. https://doi.org/10.1002/advs.202401012 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1002/advs.202401012 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.identifier.eissn 2198-3844 es_ES
dc.identifier.pmid 38884205 es_ES
dc.identifier.pmcid PMC11336962 es_ES
dc.relation.pasarela S\525645 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Research Grant Council, Hong Kong es_ES
dc.contributor.funder European Regional Development Fund es_ES


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