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dc.contributor.author | Di, Xueni | es_ES |
dc.contributor.author | RODRIGUEZ-CONCEPCION, Manuel | es_ES |
dc.date.accessioned | 2024-10-16T11:08:52Z | |
dc.date.available | 2024-10-16T11:08:52Z | |
dc.date.issued | 2023-11 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/210276 | |
dc.description.abstract | [EN] Isoprenoids are a wide family of metabolites including high-value chemicals, flavors, pigments, and drugs. Isoprenoids are particularly abundant and diverse in plants. The methyl-D-erythritol 4-phosphate (MEP) pathway produces the universal isoprenoid precursors isopentenyl diphosphate and dimethylallyl diphosphate in plant plastids for the downstream production of monoterpenes, diterpenes, and photosynthesis-related isoprenoids such as carotenoids, chlorophylls, tocopherols, phylloquinone, and plastoquinone. The enzyme deoxy-D-xylulose 5-phosphate synthase (DXS) is the first and main rate-determining enzyme of the MEP pathway. In tomato (Solanum lycopersicum), a plant with an active isoprenoid metabolism in several tissues, three genes encode DXS-like proteins (SlDXS1 to 3). Here, we show that the expression patterns of the three genes suggest distinct physiological roles without excluding that they might function together in some tissues. We also confirm that SlDXS1 and 2 are true DXS enzymes, whereas SlDXS3 lacks DXS activity. We further show that SlDXS1 and 2 co-localize in plastidial speckles and that they can be immunoprecipitated together, suggesting that they might form heterodimers in vivo in at least some tissues. These results provide novel insights for the biotechnological use of DXS isoforms in metabolic engineering strategies to up-regulate the MEP pathway flux. | es_ES |
dc.description.sponsorship | This work was funded by grants PID2020-115810GB-I00 and UToPIQ-PCI2021-121941 from Spanish MCIN/AEI/10.13039/501100011033 and European NextGeneration EU/PRTR and PRIMA programs and PROMETEU/2021/056 and AGROALNEXT/2022/067 from Generalitat Valenciana to M.R.-C. X.D. was supported by the China Scholarship Council. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation.ispartof | Plants | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Carotenoid | es_ES |
dc.subject | DXS | es_ES |
dc.subject | Heterodimer | es_ES |
dc.subject | Isoprenoid | es_ES |
dc.subject | MEP pathway | es_ES |
dc.subject | Tomato | es_ES |
dc.title | Exploring the Deoxy-D-xylulose-5-phosphate Synthase Gene Family in Tomato (Solanum lycopersicum) | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/plants12223886 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PCI2021-121941/ES/USE OF TOMATO LINES TOLERANT TO PROXIMITY SHADE TO INCREASE YIELD AND QUALITY IN INTERCROPPING AGROSYSTEMS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115810GB-I00/ES/MECANISMOS MOLECULARES DE LA BIOGENESIS DE CROMOPLASTOS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2021%2F056/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//AGROALNEXT%2F2022%2F067/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Di, X.; Rodriguez-Concepcion, M. (2023). Exploring the Deoxy-D-xylulose-5-phosphate Synthase Gene Family in Tomato (Solanum lycopersicum). Plants. 12(22). https://doi.org/10.3390/plants12223886 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/plants12223886 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 12 | es_ES |
dc.description.issue | 22 | es_ES |
dc.identifier.eissn | 2223-7747 | es_ES |
dc.identifier.pmid | 38005784 | es_ES |
dc.identifier.pmcid | PMC10675008 | es_ES |
dc.relation.pasarela | S\514163 | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | China Scholarship Council | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |