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dc.contributor.author | De Brasi-Velasco, Sabrina | es_ES |
dc.contributor.author | Sanchez-Guerrero, Antonio | es_ES |
dc.contributor.author | Castillo López Del Toro, Mª Cruz | es_ES |
dc.contributor.author | Vertommen, Didier | es_ES |
dc.contributor.author | León, José | es_ES |
dc.contributor.author | Sevilla, Francisca | es_ES |
dc.contributor.author | Jimenez, Ana | es_ES |
dc.date.accessioned | 2024-11-25T19:22:47Z | |
dc.date.available | 2024-11-25T19:22:47Z | |
dc.date.issued | 2023-07 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/212249 | |
dc.description.abstract | [EN] Abscisic acid (ABA) plays a fundamental role in plant growth and development processes such as seed germination, stomatal response or adaptation to stress, amongst others. Increases in the endogenous ABA content is recognized by specific receptors of the PYR/PYL/RCAR family that are coupled to a phosphorylation cascade targeting transcription factors and ion channels. Just like other receptors of the family, nuclear receptor PYR1 binds ABA and inhibits the activity of type 2C phosphatases (PP2Cs), thus avoiding the phosphatase-exerted inhibition on SnRK2 kinases, positive regulators which phosphorylate targets and trigger ABA signalling. Thioredoxins (TRXs) are key components of cellular redox homeostasis that regulate specific target proteins through a thiol-disulfide exchange, playing an essential role in redox homeostasis, cell survival, and growth. In higher plants, TRXs have been found in almost all cellular compartments, although its presence and role in nucleus has been less studied. In this work, affinity chromatography, Dot-blot, co-immunoprecipitation, and bimolecular fluorescence complementation assays allowed us to identify PYR1 as a new TRXo1 target in the nucleus. Studies on recombinant HisAtPYR1 oxidation-reduction with wild type and site-specific mutagenized forms showed that the receptor underwent redox regulation involving changes in the oligomeric state in which Cys30 and Cys65 residues were implied. TRXo1 was able to reduce previously-oxidized inactive PYR1, thus recovering its capacity to inhibit HAB1 phosphatase. In vivo PYR1 oligomerization was dependent on the redox state, and a differential pattern was detected in KO and over-expressing Attrxo1 mutant plants grown in the presence of ABA compared to WT plants. Thus, our findings suggest the existence of a redox regulation of TRXo1 on PYR1 that may be relevant for ABA signalling and had not been described so far. | es_ES |
dc.description.sponsorship | This study was supported by the Spanish grants MICINN-FEDER (PID2021-127335NB-I00, PID2020-112618 GB-I00 (to JL) and Network RED2018-102397-T) and S¿eneca Foundation (Excellence Project 19876/GERM/15 and project 22051/PI/22). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Redox Biology | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Abscisic acid | es_ES |
dc.subject | Oligomerization | es_ES |
dc.subject | Protein interaction | es_ES |
dc.subject | PYR1 | es_ES |
dc.subject | Redox regulation | es_ES |
dc.subject | Thioredoxin o1 | es_ES |
dc.title | Thioredoxin TRXo1 is involved in ABA perception via PYR1 redox regulation | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.envexpbot.2019.02.026 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112618GB-I00/ES/FUNCION DE LAS PROTEINAS VQ Y WRKY EN PROCESOS BIOLOGICOS MODULADOS POR OXIGENO Y OXIDO NITRICO EN PLANTAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-127335NB-I00/ES/REGULACION REDOX MEDIADA POR THIOREDOXINA: EN BUSCA DE SUS DIANAS EN PROCESOS RELACIONADOS CON ESTRES ABIOTICO/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/f SéNeCa//19876%2FGERM%2F15/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/f SéNeCa//22051%2FPI%2F22/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//RED2018-102397-T/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | De Brasi-Velasco, S.; Sanchez-Guerrero, A.; Castillo López Del Toro, MC.; Vertommen, D.; León, J.; Sevilla, F.; Jimenez, A. (2023). Thioredoxin TRXo1 is involved in ABA perception via PYR1 redox regulation. Redox Biology. 63. https://doi.org/10.1016/j.envexpbot.2019.02.026 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.envexpbot.2019.02.026 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 63 | es_ES |
dc.identifier.eissn | 2213-2317 | es_ES |
dc.relation.pasarela | S\514100 | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
dc.contributor.funder | Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia | es_ES |