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Fast and robust wave optics-based reconstruction protocol for Fourier lightfield microscopy

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Fast and robust wave optics-based reconstruction protocol for Fourier lightfield microscopy

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dc.contributor.author Incardona, Nicolo es_ES
dc.contributor.author Tolosa, Angel es_ES
dc.contributor.author Saavedra, Genaro es_ES
dc.contributor.author Martinez-Corral, Manuel es_ES
dc.contributor.author Sánchez-Ortiga, Emilio es_ES
dc.date.accessioned 2024-11-29T19:08:00Z
dc.date.available 2024-11-29T19:08:00Z
dc.date.issued 2023-02 es_ES
dc.identifier.issn 0143-8166 es_ES
dc.identifier.uri http://hdl.handle.net/10251/212494
dc.description.abstract [EN] Fourier lightfield microscopy (FLMic) is a powerful technique to record 3D images of thick dynamic samples. Belonging FLMic to the general class of computational imaging techniques, its efficiency is determined by sev-eral factors, like the optical system, the calibration process, the reconstruction algorithm, or the computation architecture. In the case of FLMic the calibration and the reconstruction algorithm should be fully adapted to the singular features of the technique. To this end, and concerning the reconstruction, we discard the use of experimental PSFs, and propose the use of a synthetic one, which is calculated on the basis of paraxial optics and taking into account the equal influence of diffraction and pixelation. Using this quite simple PSF, performing the adequate calibration and finally implementing the algorithm in GPU, we demonstrate here the possibility of obtaining 3D images with good results in terms of resolution and strong improvement in terms of computation time. In summary, and aiming to accelerate the widespread of FLMic among microscopy users and researchers, we are proposing a fast protocol fully adapted to FLMic and that is very flexible and robust against any slight misalignment or against the change of any optical element. es_ES
dc.description.sponsorship This research was funded by Grant RTI2018-099041-B-I00, which is co-founded by the Ministerio de Ciencia, Innovacion y Universidades (Spain), by the European Regional Development Fund, and by Generalitat Valenciana (Spain) under Grant PROMETEO/2019/048. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Optics and Lasers in Engineering es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Fourier lightfield microscopy es_ES
dc.subject FLMic es_ES
dc.subject 3D microscopy es_ES
dc.subject 3D Deconvolution es_ES
dc.subject GPU es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Fast and robust wave optics-based reconstruction protocol for Fourier lightfield microscopy es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.optlaseng.2022.107336 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099041-B-I00/ES/MICROSCOPIO MULTIMODAL PARA LA OBTENCION DE IMAGENES BIOMEDICAS 3D/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2019%2F048/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Incardona, N.; Tolosa, A.; Saavedra, G.; Martinez-Corral, M.; Sánchez-Ortiga, E. (2023). Fast and robust wave optics-based reconstruction protocol for Fourier lightfield microscopy. Optics and Lasers in Engineering. 161. https://doi.org/10.1016/j.optlaseng.2022.107336 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.optlaseng.2022.107336 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 161 es_ES
dc.relation.pasarela S\529449 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES


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