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Composition of free and adherent ruminal bacteria: inaccuracyof the microbial nutrient supply estimates obtained using freebacteria as reference samples and 15N as the marker

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Composition of free and adherent ruminal bacteria: inaccuracyof the microbial nutrient supply estimates obtained using freebacteria as reference samples and 15N as the marker

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dc.contributor.author González, J es_ES
dc.contributor.author Arroyo, J.M. es_ES
dc.contributor.author Ouarti, M
dc.contributor.author Guevara-González, J.
dc.contributor.author Rodríguez, C.A.
dc.contributor.author Alvir, M.R.
dc.contributor.author Moya Salvador, Vicente Javier
dc.contributor.author Piquer Querol, Olga
dc.date.accessioned 2013-04-15T08:56:10Z
dc.date.available 2013-04-15T08:56:10Z
dc.date.issued 2012
dc.identifier.issn 1751-7311
dc.identifier.uri http://hdl.handle.net/10251/27815
dc.description.abstract Previous studies have indicated that 15N enrichment of solid-associated bacteria (SAB) may be predicted from the same value in liquid-associated bacteria (LAB). The aims of this study were to confirm this and to measure the error in the nutrient supply from SAB, when LAB are used as the reference sample. For this purpose, the chemical and amino acid (AA) compositions of both the bacterial populations were studied in four experiments carried out on different groups of three rumen cannulated wethers. Diets (one in Experiments 1 and 4 and three in Experiments 2 and 3) had forage-to-concentrate ratios (dry matter (DM) basis) between 2 : 1 and 40 : 60, and were consumed at intake levels between 40 and 75 g DM/kg (BW) 0.75. The bacteria samples were isolated after continuous infusion of ( 15NH 4) 2SO 4 (40, 18, 30 and 25 mg 15N/day, in Experiments 1 to 4, respectively) for at least 14 days. In all experiments, SAB had consistently higher concentrations of organic matter (826 v. 716 g/kg DM, as average) and total lipids (192 v. 95 g/kg DM, as average) than LAB. Similar CP concentrations of both populations were observed, except a higher concentration in SAB than in LAB in Experiment 3. A consistent (in Experiment 4 only as tendency) higher AA-N/total N ratio (on average 17.5%) was observed in SAB than in LAB. The 15N enrichment in SAB was systematically lower than in LAB. On the basis of the results of all studies a close relationship was found between the 15N enrichment in SAB and LAB, which was shown irrespective of experiments. This relationship was established from Experiments 1 and 2 and the above cited previous results (n = 20; P < 0.001; R 2 = 0.996), and then confirmed from the results of Experiments 3 and 4. These relationships between SAB and LAB demonstrate that CP supply from SAB is underevaluated by, on average, 21.2% when LAB are used as the reference. This underevaluation was higher for true protein and even higher for the lipid supply (32.5% and 59.6%, respectively, as an average of the four experiments). Large differences in AA profile were observed between SAB and LAB. The prediction equation obtained using 15N as the marker may be used to correct the errors associated with the traditional use of LAB as the reference sample, and therefore to obtain more accurate estimates of the microbial nutrient supply to the ruminants. © Copyright The Animal Consortium 2011. es_ES
dc.description.sponsorship Financial support was provided by the CICYT funded Projects AGL 2001-3662, AGL 2005-01712 and AGL 2006-08300. Analyses of 15N isotope ratios were performed at the Servicio Interdepartamental de Investigacio´ n, Universidad Auto´ noma de Madrid, Spain. en_EN
dc.language Inglés es_ES
dc.publisher Cambridge University Press
dc.relation CICYT/AGL 2001-3662
dc.relation CICYT/AGL 2005-01712
dc.relation CICYT/AGL 2006-08300
dc.relation.ispartof Animal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject 15N/N es_ES
dc.subject Amino acids es_ES
dc.subject Chemical composition es_ES
dc.subject Microbial nutrient supply es_ES
dc.subject Rumen bacteria es_ES
dc.subject Amino acid es_ES
dc.subject Biological marker es_ES
dc.subject Lipid es_ES
dc.subject Nitrogen es_ES
dc.subject Animal es_ES
dc.subject Animal food es_ES
dc.subject Bacterium es_ES
dc.subject Classification es_ES
dc.subject Conference paper es_ES
dc.subject Evaluation es_ES
dc.subject Gas chromatography es_ES
dc.subject Isolation and purification es_ES
dc.subject Male es_ES
dc.subject Microbiology es_ES
dc.subject Ruminant stomach es_ES
dc.subject Sheep es_ES
dc.subject Animal Feed es_ES
dc.subject Animals es_ES
dc.subject Bacteria es_ES
dc.subject Biological Markers es_ES
dc.subject Chromatography, Gas es_ES
dc.subject Lipids es_ES
dc.subject Rumen es_ES
dc.subject Bacteria (microorganisms) es_ES
dc.subject Bovidae es_ES
dc.subject Gas
dc.title Composition of free and adherent ruminal bacteria: inaccuracyof the microbial nutrient supply estimates obtained using freebacteria as reference samples and 15N as the marker es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1017/S1751731111001807
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation González, J.; Arroyo, J.; Ouarti, M.; Guevara-González, J.; Rodríguez, C.; Alvir, M.; Moya Salvador, VJ.... (2012). Composition of free and adherent ruminal bacteria: inaccuracyof the microbial nutrient supply estimates obtained using freebacteria as reference samples and 15N as the marker. Animal. 6(3):468-475. doi:10.1017/S1751731111001807 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1017/S1751731111001807 es_ES
dc.description.upvformatpinicio 468 es_ES
dc.description.upvformatpfin 475 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 6 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 215128
dc.contributor.funder Comisión Interministerial de Ciencia y Tecnología


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