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Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars

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Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars

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dc.contributor.author Ortega Higueruelo, Francisco José es_ES
dc.contributor.author Bañuls Polo, Mª José es_ES
dc.contributor.author Sanza, Francisco J. es_ES
dc.contributor.author Casquel, Rafael es_ES
dc.contributor.author Laguna, Mari Fe es_ES
dc.contributor.author Holgado, Miguel es_ES
dc.contributor.author López-Romero, David es_ES
dc.contributor.author Barrios, Carlos A. es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.contributor.author Puchades Pla, Rosa es_ES
dc.date.accessioned 2013-04-16T07:02:50Z
dc.date.available 2013-04-16T07:02:50Z
dc.date.issued 2012
dc.identifier.issn 2079-6374
dc.identifier.uri http://hdl.handle.net/10251/27865
dc.description.abstract In this paper, label-free biosensing for antibody screening by periodic lattices of high-aspect ratio SU-8 nano-pillars (BICELLs) is presented. As a demonstration, the determination of anti-gestrinone antibodies from whole rabbit serum is carried out, and for the first time, the dissociation constant (KD = 6 nM) of antigen-antibody recognition process is calculated using this sensing system. After gestrinone antigen immobilization on the BICELLs, the immunorecognition was performed. The cells were interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR), and the dip wavenumber shift was monitored. The biosensing assay exhibited good reproducibility and sensitivity (LOD = 0.75 ng/mL). es_ES
dc.format.extent 14 es_ES
dc.language Inglés es_ES
dc.publisher MDPI es_ES
dc.relation.ispartof Biosensors es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Micro-nanofabrication es_ES
dc.subject SU-8 es_ES
dc.subject Optical interrogation es_ES
dc.subject Label-free nanobiosensing es_ES
dc.subject Gestrinone es_ES
dc.subject Immunoassay affinity constant determination es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/bios2030291
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Ortega Higueruelo, FJ.; Bañuls Polo, MJ.; Sanza, FJ.; Casquel, R.; Laguna, MF.; Holgado, M.; López-Romero, D.... (2012). Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars. Biosensors. 2:291-304. doi:10.3390/bios2030291 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://www.mdpi.com/2079-6374/2/3/291 es_ES
dc.description.upvformatpinicio 291 es_ES
dc.description.upvformatpfin 304 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 2 es_ES
dc.relation.senia 232804
dc.identifier.pmcid PMC4263551 en_EN


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