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Localization of QTLs for in vitro plant regeneration in tomato (Solanum lycopersicum L.).

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Localization of QTLs for in vitro plant regeneration in tomato (Solanum lycopersicum L.).

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dc.contributor.author Trujillo Moya, Carlos es_ES
dc.contributor.author Gisbert Domenech, María Carmen es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.contributor.author Nuez Viñals, Fernando es_ES
dc.date.accessioned 2013-04-16T09:46:33Z
dc.date.available 2013-04-16T09:46:33Z
dc.date.issued 2011
dc.identifier.issn 1471-2229
dc.identifier.uri http://hdl.handle.net/10251/27885
dc.description.abstract Background Low regeneration ability limits biotechnological breeding approaches. The influence of genotype in the regeneration response is high in both tomato and other important crops. Despite the various studies that have been carried out on regeneration genetics, little is known about the key genes involved in this process. The aim of this study was to localize the genetic factors affecting regeneration in tomato. Results We developed two mapping populations (F2 and BC1) derived from a previously selected tomato cultivar (cv. Anl27) with low regeneration ability and a high regeneration accession of the wild species Solanum pennellii (PE-47). The phenotypic assay indicated dominance for bud induction and additive effects for both the percentage of explants with shoots and the number of regenerated shoots per explant. Two linkage maps were developed and six QTLs were identified on five chromosomes (1, 3, 4, 7 and 8) in the BC1 population by means of the Interval Mapping and restricted Multiple QTL Mapping methods. These QTLs came from S. pennellii, with the exception of the minor QTL located on chromosome 8, which was provided by cv. Anl27. The main QTLs correspond to those detected on chromosomes 1 and 7. In the F2 population, a QTL on chromosome 7 was identified on a similar region as that detected in the BC1 population. Marker segregation distortion was observed in this population in those areas where the QTLs of BC1 were detected. Furthermore, we located two tomato candidate genes using a marker linked to the high regeneration gene: Rg-2 (a putative allele of Rg-1) and LESK1, which encodes a serine/threonine kinase and was proposed as a marker for regeneration competence. As a result, we located a putative allele of Rg-2 in the QTL detected on chromosome 3 that we named Rg-3. LESK1, which is also situated on chromosome 3, is outside Rg-3. In a preliminary exploration of the detected QTL peaks, we found several genes that may be related to regeneration. Conclusions In this study we have identified new QTLs related to the complex process of regeneration from tissue culture. We have also located two candidate genes, discovering a putative allele of the high regeneration gene Rg-1 in the QTL on chromosome 3. The identified QTLs could represent a significant step toward the understanding of this process and the identification of other related candidate genes. It will also most likely facilitate the development of molecular markers for use in gene isolation. es_ES
dc.format.extent 13 es_ES
dc.language Inglés es_ES
dc.publisher BioMed Central es_ES
dc.relation.ispartof BMC Plant Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Agrobacterium-Mediated transformation es_ES
dc.subject Tissue-Culture response es_ES
dc.subject Shoot regeneration es_ES
dc.subject Lycopersicon-Esculentum es_ES
dc.subject Arabidopsis-Thaliana es_ES
dc.subject Genetic-Analysis es_ES
dc.subject Cucumis-Melo es_ES
dc.subject Major Genes es_ES
dc.subject Markers es_ES
dc.subject Capacity es_ES
dc.subject.classification GENETICA es_ES
dc.title Localization of QTLs for in vitro plant regeneration in tomato (Solanum lycopersicum L.). es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/1471-2229-11-140
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Trujillo Moya, C.; Gisbert Domenech, MC.; Vilanova Navarro, S.; Nuez Viñals, F. (2011). Localization of QTLs for in vitro plant regeneration in tomato (Solanum lycopersicum L.). BMC Plant Biology. 11:140-152. doi:10.1186/1471-2229-11-140 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://www.biomedcentral.com/content/pdf/1471-2229-11-140.pdf es_ES
dc.description.upvformatpinicio 140 es_ES
dc.description.upvformatpfin 152 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 11 es_ES
dc.relation.senia 206604
dc.identifier.pmid 22014149 es_ES
dc.identifier.pmcid PMC3209458 es_ES


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