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Aluminum Oxide Nanoparticles as Carriers and Adjuvants for Eliciting Antibodies from Non-immunogenic Haptens

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Aluminum Oxide Nanoparticles as Carriers and Adjuvants for Eliciting Antibodies from Non-immunogenic Haptens

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dc.contributor.author Maquieira Catala, Ángel es_ES
dc.contributor.author Brun Sánchez, Eva María es_ES
dc.contributor.author Garcés García, Marta es_ES
dc.contributor.author Puchades Pla, Rosa es_ES
dc.date.accessioned 2013-04-18T11:52:21Z
dc.date.issued 2012
dc.identifier.issn 0003-2700
dc.identifier.uri http://hdl.handle.net/10251/28038
dc.description.abstract [EN] Functionalized nanomaterials have applications yet to be discovered, especially in the biological field and, in particular, the in vivo production of antibodies. In this paper, we show that aluminum oxide nanoparticles that are covalently coupled to haptens (small molecular mass compounds) activate the immune system, eliciting antibodies (polyclonal and monoclonal) specific for the herbicide atrazine, the antibiotic sulfasalazine, and the vitamin biotin in mice and rabbits. The particles play the role of carrier and adjuvant, with the immune response being dependent on size and crystallinity. The affinity constants of the antibodies are similar to those reached with traditional immunization strategies based on the use of carrier proteins. This approach has not been previously described, being of scientific and practical interest, as it can lead to immunogens safer than the conventional ones, potentially applicable to human vaccination. In addition, the useful advantages of this technique include the stability of the metallic particles, the synthesis of immunogens in organic media, the versatility of particle derivatization, the ease of purification, the full chemical characterization of immunogens, the lack of a requirement for an adjuvant, the reduction of the cross reactivity, and the low cost of materials. Also, the analytical performances of the immunoassays developed using these antibodies are comparable to those obtained by the standard protocols. Analytical applications of the developed ELISAs were fully demonstrated. Characterization (IgG nature, affinity constants, etc.) of the immunoreagents were also fully assessed. © 2012 American Chemical Society. es_ES
dc.description.sponsorship The authors are thankful to S. Gee (Entomology Department, UC Davis, CA, USA) for aiding previous exploratory work, to T. Corns and M. Cambra (IVIA., Valencia, Spain) for managing the animal experiments and obtaining the monoclonal antibodies, to M. Plebanski (Department of Immunology, Monash University, Australia) for his valuable aid in planning the research, and to G. Gonzalez-Sapienza (Department of Immunology, Universidad de la Republica, Montevideo, Uruguay) for the sharp recommendations and critical evaluation of the results. This research was partially supported by Prometeo 2010/008 (Generalitat Valenciana) and FEDER MICINN CTQ2010-15943 project. en_EN
dc.language Inglés es_ES
dc.publisher American Chemical Society es_ES
dc.relation.ispartof Analytical Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Affinity constants es_ES
dc.subject Aluminum oxides es_ES
dc.subject Analytical applications es_ES
dc.subject Analytical performance es_ES
dc.subject Biological fields es_ES
dc.subject Carrier proteins es_ES
dc.subject Chemical characterization es_ES
dc.subject Cross reactivity es_ES
dc.subject Crystallinities es_ES
dc.subject Derivatizations es_ES
dc.subject Functionalized es_ES
dc.subject Immune response es_ES
dc.subject Immune systems es_ES
dc.subject Immunogens es_ES
dc.subject Immunoreagents es_ES
dc.subject In-vivo es_ES
dc.subject Low costs es_ES
dc.subject Metallic particles es_ES
dc.subject Organic media es_ES
dc.subject Polyclonal es_ES
dc.subject Standard protocols es_ES
dc.subject Antigens es_ES
dc.subject Chemical stability es_ES
dc.subject Herbicides es_ES
dc.subject Nanoparticles es_ES
dc.subject Oxides es_ES
dc.subject Antibodies es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Aluminum Oxide Nanoparticles as Carriers and Adjuvants for Eliciting Antibodies from Non-immunogenic Haptens es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1021/ac3020998
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F008/ES/AYUDA PARA EL GRUPO SYM-IDM/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//CTQ2010-15943/ES/ESTUDIO DE NUEVAS VIAS DE DESARROLLO DE BIOMEMS PARA SCREENING MASIVO. DEMOSTRACION DE CONCEPTO COMO HERRAMIENTA DE ANALISIS APLICABLE EN "OMICAS"./ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Maquieira Catala, Á.; Brun Sánchez, EM.; Garcés García, M.; Puchades Pla, R. (2012). Aluminum Oxide Nanoparticles as Carriers and Adjuvants for Eliciting Antibodies from Non-immunogenic Haptens. Analytical Chemistry. 84:9340-9348. https://doi.org/10.1021/ac3020998 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://pubs.acs.org/doi/ipdf/10.1021/ac3020998 es_ES
dc.description.upvformatpinicio 9340 es_ES
dc.description.upvformatpfin 9348 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 84 es_ES
dc.relation.senia 232759
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Generalitat Valenciana es_ES


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