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A Label-Free Interdigitated Microelectrodes Immunosensor for Pesticide Detection

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A Label-Free Interdigitated Microelectrodes Immunosensor for Pesticide Detection

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dc.contributor.author Marrakchi, Mouna es_ES
dc.contributor.author Campos Sánchez, Inmaculada es_ES
dc.contributor.author Helali, S. es_ES
dc.contributor.author Mejri, N. es_ES
dc.contributor.author Soto Camino, Juan es_ES
dc.contributor.author González Martínez, Miguel Ángel es_ES
dc.contributor.author Hamdi, M. es_ES
dc.contributor.author Abdelghani, A. es_ES
dc.date.accessioned 2013-04-23T12:56:32Z
dc.date.issued 2011
dc.identifier.issn 1546-198X
dc.identifier.uri http://hdl.handle.net/10251/28160
dc.description.abstract [EN] A sensitive, simple and label-free immunosensor for atrazine detection was developed. The immunosensor was based on antibody physisorption as immobilization technique. This technique was proposed as a cost-effective gold immuno-functionalization strategy alternative to SAM-based strategy for pesticide detection. Each step of immunosensor construction was characterized by electrochemical impedance spectroscopy, and the antibody-atrazine interaction was measured in a dynamic range from 10 to 150 ng/mL. This method was highly selective for atrazine and propazine, showing weak or no cross-reactivity to other similar compounds like terbutylazine. A limit detection of 10 ng/mL of atrazine was detected in PBS buffer with a good reproductibility. Lower limit detection in tap water was obtained. es_ES
dc.description.sponsorship This work was financially supported by the PCI cooperation project between Spain and Tunisia. The authors thank Dr. F. Del Campo and Dr. E. Baldrich from CNM-Barcelona (Spain) for the interdigitated microelectrode fabrication. Pr. A. Abdelghani has to thanks the NATO (North Atlantic Treaty Organization) for the material donation. en_EN
dc.language Inglés es_ES
dc.publisher American Scientific Publishers es_ES
dc.relation.ispartof Sensor letters es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Atrazine es_ES
dc.subject Electrochemical impedance spectroscopy es_ES
dc.subject Immunosensor es_ES
dc.subject Physisorption es_ES
dc.subject Cross-reactivity es_ES
dc.subject Dynamic range es_ES
dc.subject Immobilization technique es_ES
dc.subject Interdigitated microelectrodes es_ES
dc.subject Label free es_ES
dc.subject Label-free immunosensor es_ES
dc.subject Lower limits es_ES
dc.subject Pesticide detection es_ES
dc.subject Propazine es_ES
dc.subject Reproductibility es_ES
dc.subject Tap water es_ES
dc.subject Antibodies es_ES
dc.subject Herbicides es_ES
dc.subject Microelectrodes es_ES
dc.subject Immunosensors es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.subject.classification QUIMICA INORGANICA es_ES
dc.title A Label-Free Interdigitated Microelectrodes Immunosensor for Pesticide Detection es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1166/sl.2011.1800
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Marrakchi, M.; Campos Sánchez, I.; Helali, S.; Mejri, N.; Soto Camino, J.; González Martínez, MÁ.; Hamdi, M.... (2011). A Label-Free Interdigitated Microelectrodes Immunosensor for Pesticide Detection. Sensor letters. 9:2203-2206. doi:10.1166/sl.2011.1800 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://www.ingentaconnect.com/content/asp/senlet/2011/00000009/00000006/art00024?token=00561ef47838269a06c30b41333c4a2f7a3f6a2d3b7446477d3f34704f6d62222c227e372530332976d8f2 es_ES
dc.description.upvformatpinicio 2203 es_ES
dc.description.upvformatpfin 2206 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.relation.senia 232805


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