dc.contributor.author |
Marrakchi, Mouna
|
es_ES |
dc.contributor.author |
Campos Sánchez, Inmaculada
|
es_ES |
dc.contributor.author |
Helali, S.
|
es_ES |
dc.contributor.author |
Mejri, N.
|
es_ES |
dc.contributor.author |
Soto Camino, Juan
|
es_ES |
dc.contributor.author |
González Martínez, Miguel Ángel
|
es_ES |
dc.contributor.author |
Hamdi, M.
|
es_ES |
dc.contributor.author |
Abdelghani, A.
|
es_ES |
dc.date.accessioned |
2013-04-23T12:56:32Z |
|
dc.date.issued |
2011 |
|
dc.identifier.issn |
1546-198X |
|
dc.identifier.uri |
http://hdl.handle.net/10251/28160 |
|
dc.description.abstract |
[EN] A sensitive, simple and label-free immunosensor for atrazine detection was developed. The immunosensor was based on antibody physisorption as immobilization technique. This technique was proposed as a cost-effective gold immuno-functionalization strategy alternative to SAM-based strategy for pesticide detection. Each step of immunosensor construction was characterized by electrochemical impedance spectroscopy, and the antibody-atrazine interaction was measured in a dynamic range from 10 to 150 ng/mL. This method was highly selective for atrazine and propazine, showing weak or no cross-reactivity to other similar compounds like terbutylazine. A limit detection of 10 ng/mL of atrazine was detected in PBS buffer with a good reproductibility. Lower limit detection in tap water was obtained. |
es_ES |
dc.description.sponsorship |
This work was financially supported by the PCI cooperation project between Spain and Tunisia. The authors thank Dr. F. Del Campo and Dr. E. Baldrich from CNM-Barcelona (Spain) for the interdigitated microelectrode fabrication. Pr. A. Abdelghani has to thanks the NATO (North Atlantic Treaty Organization) for the material donation. |
en_EN |
dc.language |
Inglés |
es_ES |
dc.publisher |
American Scientific Publishers |
es_ES |
dc.relation.ispartof |
Sensor letters |
es_ES |
dc.rights |
Reserva de todos los derechos |
es_ES |
dc.subject |
Atrazine |
es_ES |
dc.subject |
Electrochemical impedance spectroscopy |
es_ES |
dc.subject |
Immunosensor |
es_ES |
dc.subject |
Physisorption |
es_ES |
dc.subject |
Cross-reactivity |
es_ES |
dc.subject |
Dynamic range |
es_ES |
dc.subject |
Immobilization technique |
es_ES |
dc.subject |
Interdigitated microelectrodes |
es_ES |
dc.subject |
Label free |
es_ES |
dc.subject |
Label-free immunosensor |
es_ES |
dc.subject |
Lower limits |
es_ES |
dc.subject |
Pesticide detection |
es_ES |
dc.subject |
Propazine |
es_ES |
dc.subject |
Reproductibility |
es_ES |
dc.subject |
Tap water |
es_ES |
dc.subject |
Antibodies |
es_ES |
dc.subject |
Herbicides |
es_ES |
dc.subject |
Microelectrodes |
es_ES |
dc.subject |
Immunosensors |
es_ES |
dc.subject.classification |
QUIMICA ANALITICA |
es_ES |
dc.subject.classification |
QUIMICA INORGANICA |
es_ES |
dc.title |
A Label-Free Interdigitated Microelectrodes Immunosensor for Pesticide Detection |
es_ES |
dc.type |
Artículo |
es_ES |
dc.embargo.lift |
10000-01-01 |
|
dc.embargo.terms |
forever |
es_ES |
dc.identifier.doi |
10.1166/sl.2011.1800 |
|
dc.rights.accessRights |
Cerrado |
es_ES |
dc.contributor.affiliation |
Universitat Politècnica de València. Departamento de Química - Departament de Química |
es_ES |
dc.description.bibliographicCitation |
Marrakchi, M.; Campos Sánchez, I.; Helali, S.; Mejri, N.; Soto Camino, J.; González Martínez, MÁ.; Hamdi, M.... (2011). A Label-Free Interdigitated Microelectrodes Immunosensor for Pesticide Detection. Sensor letters. 9:2203-2206. doi:10.1166/sl.2011.1800 |
es_ES |
dc.description.accrualMethod |
S |
es_ES |
dc.relation.publisherversion |
http://www.ingentaconnect.com/content/asp/senlet/2011/00000009/00000006/art00024?token=00561ef47838269a06c30b41333c4a2f7a3f6a2d3b7446477d3f34704f6d62222c227e372530332976d8f2 |
es_ES |
dc.description.upvformatpinicio |
2203 |
es_ES |
dc.description.upvformatpfin |
2206 |
es_ES |
dc.type.version |
info:eu-repo/semantics/publishedVersion |
es_ES |
dc.description.volume |
9 |
es_ES |
dc.relation.senia |
232805 |
|