Perez Garnes, M.; González García, C.; Moratal Pérez, D.; Rico Tortosa, PM.; Salmerón Sánchez, M. (2011). Fibronectin distribution on demixed nanoscale topographies. International Journal of Artificial Organs. 34(1):54-63. https://doi.org/10.5301/ijao.2011.6316
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/28173
Title:
|
Fibronectin distribution on demixed nanoscale topographies
|
Author:
|
Perez Garnes, Manuel
González García, Cristina
Moratal Pérez, David
Rico Tortosa, Patricia María
Salmerón Sánchez, Manuel
|
UPV Unit:
|
Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica
Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada
|
Issued date:
|
|
Abstract:
|
[EN] Purpose: It is known that surface nanotopography influences cell adhesion and differentiation. Our aim is to analyze the effect of nanoscale topography on fibronectin adsorption and, afterwards, on cell adhesion in ...[+]
[EN] Purpose: It is known that surface nanotopography influences cell adhesion and differentiation. Our aim is to analyze the effect of nanoscale topography on fibronectin adsorption and, afterwards, on cell adhesion in order to rationalize the cell-material interaction by focusing on the state of the intermediate layer of adsorbed fibronectin at the material interphase. Methods: Nanotopographic surfaces were produced by demixing of thin film polymer blends - PLLA and PS - during a high speed spin-casting process. Fibronectin (FN) was adsorbed on the different nanotopographies and the protein distribution was directly observed by atomic force microscopy (AFM). The fraction of the surface covered by the protein was quantified by image analysis, as well as the distribution of FN between peaks and valleys. Focal adhesion protein -vinculin- was immunostained and quantified by image analysis on the different nanoscale surfaces. Results: Different nanoscale domains were obtained by changing the composition of the system within a height range of 3 nm to 30 nm. FN tends to adsorb on the peaks of nanoisland topographies, especially in compositions that did not enhance cell adhesion. Moreover, protein distribution between valleys and peaks alters the size of focal adhesion plaques, which grew larger on surfaces with an even distribution of fibronectin. Conclusions: Our results suggest that the surface nanotopography is a key material property capable of influencing protein adsorption. Additionally, the distribution of the protein on the different samples was correlated to the initial ability of cells to adhere in terms of the size of the focal plaques. © 2011 Wichtig Editore.
[-]
|
Subjects:
|
Fibronectin
,
Polymer
,
Vinculin
,
Article
,
Atomic force microscopy
,
Cell adhesion
,
Cell differentiation
,
Cell interaction
,
Film
,
Human
,
Human cell
,
Image analysis
,
Immunohistochemistry
,
Nanoanalysis
,
Protein localization
,
Surface nanotopography
,
Topography
,
3T3 Cells
,
Adsorption
,
Animals
,
Fibronectins
,
Focal Adhesions
,
Humans
,
Image Processing, Computer-Assisted
,
Lactic Acid
,
Mice
,
Microscopy, Atomic Force
,
Nanotechnology
,
Polymers
,
Polystyrenes
,
Surface Properties
,
Tissue Scaffolds
|
Copyrigths:
|
Cerrado |
Source:
|
International Journal of Artificial Organs. (issn:
0391-3988
)
|
DOI:
|
10.5301/ijao.2011.6316
|
Publisher:
|
Wichtig Editore
|
Publisher version:
|
http://www.artificial-organs.com/article/fibronectin-distribution-on-demixed-nanoscale-topographies-ijao-10-00143
|
Project ID:
|
info:eu-repo/grantAgreement/MICINN//MAT2009-14440-C02-01/ES/Dinamica De Las Proteinas De La Matriz En La Interfase Celula-Material/
info:eu-repo/grantAgreement/MICINN//TEC2009-14128/ES/Analisis Microestructural, Mecanico Y Del Crecimiento Celular En Soportes Macroporosos Para Ingenieria Tisular Osea Mediante Tratamiento De Imagen./
|
Thanks:
|
This studied was funded by the Spanish Ministry of Science and Innovation through MAT2009-14440-C02-01 and TEC2009-14128 grants. CIBER-BBN is an initiative funded by the VI National R&D&D&i Plan 2008-2011, Iniciativa Ingenio ...[+]
This studied was funded by the Spanish Ministry of Science and Innovation through MAT2009-14440-C02-01 and TEC2009-14128 grants. CIBER-BBN is an initiative funded by the VI National R&D&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. This work was supported by funds for research in the field of Regenerative Medicine through the collaboration agreement with the Conselleria de Sanidad (Generalitat Valenciana), and the Instituto de Salud Carlos III.
[-]
|
Type:
|
Artículo
|