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Multiplex DNA Detection of Food Allergens on a Digital Versatile Disk

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Multiplex DNA Detection of Food Allergens on a Digital Versatile Disk

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dc.contributor.author Tortajada Genaro, Luis Antonio es_ES
dc.contributor.author Santiago Felipe, Sara es_ES
dc.contributor.author Morais Ezquerro, Sergi Beñat es_ES
dc.contributor.author GABALDÓN, JOSE ANTONIO es_ES
dc.contributor.author Puchades Pla, Rosa es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.date.accessioned 2013-04-26T12:53:56Z
dc.date.issued 2012-01-11
dc.identifier.issn 0021-8561
dc.identifier.uri http://hdl.handle.net/10251/28271
dc.description.abstract The development of a DNA microarray method on a digital versatile disk (DVD) is described for the simultaneous detection of traces of hazelnut (Corylus avellana L.), peanut (Arachis hypogaea), and soybean (Glycine max) in foods. After DNA extraction, multiplex PCR was set up using S'-labeled specific primers for Cor a 1, Ar h 2, and Le genes, respectively. Digoxin-labeled PCR products were detected by hybridization with S'-biotinylated probes immobilized on a streptavidin-modified DVD surface. The reaction product attenuates the signal intensity of the laser that reached the DVD drive used as detector, correlating well with the amount of amplified sequence. Analytical performances showed a detection limit of 1 mu g/g and good assay reproducibility (RSD 8%), suitable for the simultaneous detection of the three targeted allergens. The developed methodology was tested with several commercially available foodstuffs, demonstrating its applicability. The results were in good agreement, in terms of sensitivity and reproducibility, with those obtained with ELISA, PCR-gel agarose electrophoresis, and RT-PCR. es_ES
dc.description.sponsorship This research was funded through projects FEDER MICINN CTQ2010-15943 and by Generalitat Valenciana (GV/2009/028 and PROMETEO/2010/008). The Spanish Ministerio de Educacion y Ciencia provided S.S.-F. with a grant for her Ph.D. studies. en_EN
dc.language Español es_ES
dc.publisher American Chemical Society es_ES
dc.relation MICINN CTQ2010-15943 es_ES
dc.relation Generalitat Valenciana GV/2009/028 PROMETEO/2010/008 es_ES
dc.relation Spanish Ministerio de Educacion y Ciencia es_ES
dc.relation.ispartof Journal of Agricultural and Food Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Allergen es_ES
dc.subject DNA microarraying es_ES
dc.subject DVD es_ES
dc.subject Food es_ES
dc.subject Hazelnut es_ES
dc.subject Peanut es_ES
dc.subject Soybean es_ES
dc.subject Amino acids es_ES
dc.subject Antigens es_ES
dc.subject Data storage equipment es_ES
dc.subject Detectors es_ES
dc.subject DNA es_ES
dc.subject Electrophoresis es_ES
dc.subject Genes es_ES
dc.subject Multiplexing es_ES
dc.subject Nitrogen fixation es_ES
dc.subject Oilseeds es_ES
dc.subject Proteins es_ES
dc.subject Surface reactions es_ES
dc.subject Videodisks es_ES
dc.subject Polymerase chain reaction es_ES
dc.subject Plant antigen es_ES
dc.subject Article es_ES
dc.subject Chemistry es_ES
dc.subject Evaluation es_ES
dc.subject Food allergy es_ES
dc.subject Genetics es_ES
dc.subject Human es_ES
dc.subject Instrumentation es_ES
dc.subject Methodology es_ES
dc.subject Multiplex polymerase chain reaction es_ES
dc.subject Allergens es_ES
dc.subject Arachis hypogaea es_ES
dc.subject Corylus es_ES
dc.subject Food Hypersensitivity es_ES
dc.subject Humans es_ES
dc.subject Soybeans es_ES
dc.subject Corylus avellana es_ES
dc.subject Glycine max es_ES
dc.subject Plant
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Multiplex DNA Detection of Food Allergens on a Digital Versatile Disk es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1021/jf2037032
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Tortajada Genaro, LA.; Santiago Felipe, S.; Morais Ezquerro, SB.; Gabaldón, JA.; Puchades Pla, R.; Maquieira Catala, Á. (2012). Multiplex DNA Detection of Food Allergens on a Digital Versatile Disk. Journal of Agricultural and Food Chemistry. 60(1):36-43. doi:10.1021/jf2037032 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1021/jf2037032 es_ES
dc.description.upvformatpinicio 36 es_ES
dc.description.upvformatpfin 43 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 60 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 212779


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