Agostini, A.; Mondragón Martínez, L.; Pascual Vidal, L.; Aznar Gimeno, E.; Coll Merino, MC.; Martínez Mañez, R.; Sancenón Galarza, F.... (2012). Design of enzyme-mediated controlled release systems based on silica mesoporous supports capped with ester-glycol groups. Langmuir. 28:14766-14776. https://doi.org/10.1021/la303161e
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/28283
Title:
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Design of enzyme-mediated controlled release systems based on silica mesoporous supports capped with ester-glycol groups
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Author:
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Agostini, Alessandro
Mondragón Martínez, Laura
Pascual Vidal, Lluís
Aznar Gimeno, Elena
Coll Merino, Mª Carmen
Martínez Mañez, Ramón
Sancenón Galarza, Félix
Soto Camino, Juan
Marcos Martínez, María Dolores
Amoros del Toro, Pedro
Costero, Ana M.
Parra, Margarita
Gil, Salvador
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UPV Unit:
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Universitat Politècnica de València. Departamento de Química - Departament de Química
Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic
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Issued date:
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Abstract:
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[EN] An ethylene glycol-capped hybrid material for the controlled release of molecules in the presence of esterase enzyme has been prepared. The final organic-inorganic hybrid solid S1 was synthesized by a two-step procedure. ...[+]
[EN] An ethylene glycol-capped hybrid material for the controlled release of molecules in the presence of esterase enzyme has been prepared. The final organic-inorganic hybrid solid S1 was synthesized by a two-step procedure. In the first step, the pores of an inorganic MCM-41 support (in the form of nanoparticles) were loaded with [Ru(bipy) 3]Cl 2 complex, and then, in the second step, the pore outlets were functionalized with ester glycol moieties that acted as molecular caps. In the absence of an enzyme, release of the complex from aqueous suspensions of S1 at pH 8.0 is inhibited due to the steric hindrance imposed by the bulky ester glycol moieties. Upon addition of esterase enzyme, delivery of the ruthenium complex was observed due to enzymatic hydrolysis of the ester bond in the anchored ester glycol derivative, inducing the release of oligo(ethylene glycol) fragments. Hydrolysis of the ester bond results in size reduction of the appended group, therefore allowing delivery of the entrapped cargo. The S1 nanoparticles were not toxic for cells, as demonstrated by cell viability assays with HeLa and MCF-7 cell lines, and were found to be associated with lysosomes, as shown by confocal microscopy. However, when S1 nanoparticles were filled with the cytotoxic drug camptothecin (S1-CPT), S1-CPT-treated cells undergo cell death as a result of S1-CPT cell internalization and subsequent cellular enzyme-mediated hydrolysis and aperture of the molecular gate that induced the release of the camptothecin cargo. These findings point to a possible therapeutic application of these nanoparticles. © 2012 American Chemical Society.
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Subjects:
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Aqueous suspensions
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Camptothecin (CPT)
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Cell internalization
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Cell viability
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Controlled release
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Controlled release systems
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Cytotoxic drugs
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Enzyme-mediated hydrolysis
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Ester bonds
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Esterase enzymes
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Functionalized
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Glycol derivatives
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MCF-7 cells
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MCM-41 supports
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Mesoporous support
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Molecular cap
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Molecular gates
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Oligo(ethylene glycol)
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Organic-inorganic hybrid solids
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Ruthenium complexes
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Size reductions
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Steric hindrances
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Therapeutic Application
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Two-step procedure
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Body fluids
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Cell culture
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Cell death
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Chlorine compounds
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Confocal microscopy
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Cytotoxicity
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Enzymatic hydrolysis
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Esterification
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Ethylene glycol
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Hybrid materials
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Nanoparticles
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Ruthenium
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Ruthenium compounds
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Silica
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Suspensions (fluids)
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Esters
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Copyrigths:
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Cerrado |
Source:
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Langmuir. (issn:
0743-7463
)
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DOI:
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10.1021/la303161e
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Publisher:
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American Chemical Society
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Publisher version:
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http://pubs.acs.org/doi/pdf/10.1021/la303161e
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Project ID:
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info:eu-repo/grantAgreement/MICINN//MAT2009-14564-C04/
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Thanks:
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We thank the Spanish Government (Projects MAT2009-14564-C04 and SAF2010-15512) and the Generalitat Valencia (Project PROMETEO/2009/016) for support. A.A. and L.M. thank the Generalitat Valenciana for their Santiago Grisolia ...[+]
We thank the Spanish Government (Projects MAT2009-14564-C04 and SAF2010-15512) and the Generalitat Valencia (Project PROMETEO/2009/016) for support. A.A. and L.M. thank the Generalitat Valenciana for their Santiago Grisolia fellowship and VALI+D postdoctoral contract, respectively. We thank Eva Maria Lafuente Villarreal from the confocal microscopy service from CIPF for technical support.
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Type:
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Artículo
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