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GoldenBraid: an iterative simple standardized cloning system system for standardized assembly assembly of recyclable reusable genetic modules

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GoldenBraid: an iterative simple standardized cloning system system for standardized assembly assembly of recyclable reusable genetic modules

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dc.contributor.author Sarrión Perdigones, Manuel Alejandro es_ES
dc.contributor.author Juárez Ortega, Paloma es_ES
dc.contributor.author Fernández Del Carmen, María Asunción es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.date.accessioned 2013-04-30T14:17:54Z
dc.date.available 2013-04-30T14:17:54Z
dc.date.issued 2011
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10251/28362
dc.description.abstract Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized assembly system based on type IIS restriction enzymes that allows the indefinite growth of reusable gene modules made of standardized DNA pieces. The GB system consists of a set of four destination plasmids (pDGBs) designed to incorporate multipartite assemblies made of standard DNA parts and to combine them binarily to build increasingly complex multigene constructs. The relative position of type IIS restriction sites inside pDGB vectors introduces a double loop (¿braid¿) topology in the cloning strategy that allows the indefinite growth of composite parts through the succession of iterative assembling steps, while the overall simplicity of the system is maintained. We propose the use of GoldenBraid as an assembly standard for Plant Synthetic Biology. For this purpose we have GB-adapted a set of binary plasmids for A. tumefaciens-mediated plant transformation. Fast GB-engineering of several multigene T-DNAs, including two alternative modules made of five reusable devices each, and comprising a total of 19 basic parts are also described. es_ES
dc.description.sponsorship This work was supported by the Spanish Ministry of Science and Innovation grants BIO2008-03434 and BIO2010-15384. A. Sarrion-Perdigones is a recipient of a FPI fellowship of the Spanish Ministry of Science and Innovation and P. Juarez is a recipient of a FPU fellowship from the Spanish Ministry of Education. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS ONE es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title GoldenBraid: an iterative simple standardized cloning system system for standardized assembly assembly of recyclable reusable genetic modules es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.pone.0021622
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2008-03434/ES/EL FRUTO DE TOMATE COMO BIOFACTORIA DE PROTEINAS INMUNOTERAPEUTICAS ORALES: PRODUCCION DE ANTICUERPOS FRENTE A ROTAVIRUS/ / es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2010-15384/ES/FABRICANDO TOMATES SALUDABLES: BIOPIEZAS PARA INTRAGENESIS Y MOLECULAR FARMING EN SOLANACEAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Sarrión Perdigones, MA.; Juárez Ortega, P.; Fernandez Del Carmen, MA.; Granell Richart, A.; Orzáez Calatayud, DV. (2011). GoldenBraid: an iterative simple standardized cloning system system for standardized assembly assembly of recyclable reusable genetic modules. PLoS ONE. 7(6):21622-21622. doi:10.1371/journal.pone.0021622 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://www.plosone.org/article/info:doi/10.1371/journal.pone.0021622 es_ES
dc.description.upvformatpinicio 21622 es_ES
dc.description.upvformatpfin 21622 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 212705
dc.identifier.pmid 21750718 en_EN
dc.identifier.pmcid PMC3131274 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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