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Characterization of constricted fruit (ctf) Mutant Uncovers a Role for AtMYB117/LOF1 in Ovule and Fruit Development in Arabidopsis thaliana

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Characterization of constricted fruit (ctf) Mutant Uncovers a Role for AtMYB117/LOF1 in Ovule and Fruit Development in Arabidopsis thaliana

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dc.contributor.author Gómez Jiménez, Maria Dolores es_ES
dc.contributor.author Urbez Lagunas, Cristina es_ES
dc.contributor.author Perez Amador, Miguel Angel es_ES
dc.contributor.author Carbonell Gisbert, Juan es_ES
dc.date.accessioned 2013-05-02T10:38:34Z
dc.date.available 2013-05-02T10:38:34Z
dc.date.issued 2011-04-13
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10251/28387
dc.description.abstract Pistil and fruit morphogenesis is the result of a complex gene network that is not yet fully understood. A search for novel genes is needed to make a more comprehensive model of pistil and fruit development. Screening for mutants with alterations in fruit morphology generated by an activation tagging strategy resulted in the isolation of the ctf (constricted fruit) mutant. It is characterized by a) small and wrinkled fruits, with an enlarged replum, an amorphous structure of the septum and an irregular distribution of ovules and seeds; b) ectopic carpelloid structures in sepals bearing ovule-like structures and c) dwarf plants with curled rosette leaves. The overexpressed gene in ctf was AtMYB117, also named LOF1 (LATERAL ORGAN FUSION1). AtMYB117/LOF1 transcripts were localized in boundary regions of the vegetative shoot apical meristem and leaf primordia and in a group of cells in the adaxial base of petioles and bracts. Transcripts were also detected in the boundaries between each of the four floral whorls and during pistil development in the inner of the medial ridges, the placenta, the base of the ovule primordia, the epidermis of the developing septum and the outer cell layers of the ovule funiculi. Analysis of changes of expression of pistil-related genes in the ctf mutant showed an enhancement of SHATTERPROOF1 (SHP1) and SHP2 expression. All these results suggest that AtMYB117/LOF1 is recruited by a variety of developmental programs for the establishment of boundary regions, including the development of floral organs and the initiation of ovule outgrowth. es_ES
dc.description.sponsorship This work was funded by the Spanish Ministry of Science and Innovation (grants BIO2005-07156-C02-01 and BIO2008-01039). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS ONE es_ES
dc.rights Reconocimiento (by) es_ES
dc.title Characterization of constricted fruit (ctf) Mutant Uncovers a Role for AtMYB117/LOF1 in Ovule and Fruit Development in Arabidopsis thaliana es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.pone.0018760
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2005-07156-C02-01 / es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2008-01039/ES/ANALISIS DE LA COORDINACION DE LOS PROGRAMAS DE DESARROLLO DE SEMILLAS Y FRUTOS DURANTE LA FRUCTIFICACION/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Gómez Jiménez, MD.; Urbez Lagunas, C.; Perez Amador, MA.; Carbonell Gisbert, J. (2011). Characterization of constricted fruit (ctf) Mutant Uncovers a Role for AtMYB117/LOF1 in Ovule and Fruit Development in Arabidopsis thaliana. PLoS ONE. 6(4):18760-18760. doi:10.1371/journal.pone.0018760 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018760 es_ES
dc.description.upvformatpinicio 18760 es_ES
dc.description.upvformatpfin 18760 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 6 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 213030
dc.identifier.pmid 21533201 en_EN
dc.identifier.pmcid PMC3076444 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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