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A permeable cuticle is associated with the release of reactive oxygen species and induction of innate immunity

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A permeable cuticle is associated with the release of reactive oxygen species and induction of innate immunity

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dc.contributor.author L'Haridon, Floriane es_ES
dc.contributor.author Besson-Bard, Angelique es_ES
dc.contributor.author Binda, Matteo es_ES
dc.contributor.author Serrano Mateo, Mario es_ES
dc.contributor.author Abou-Mansour, Eliane es_ES
dc.contributor.author Balet, Francine es_ES
dc.contributor.author Schoonbeek, Henk-Jan es_ES
dc.contributor.author Hess, Stephane es_ES
dc.contributor.author Mir Moreno, Ricardo es_ES
dc.contributor.author Leon Ramos, Jose es_ES
dc.contributor.author Lamotte, Olivier es_ES
dc.contributor.author Metraux, Jean-Pierre es_ES
dc.date.accessioned 2013-05-06T12:20:07Z
dc.date.available 2013-05-06T12:20:07Z
dc.date.issued 2011
dc.identifier.issn 1553-7366
dc.identifier.uri http://hdl.handle.net/10251/28578
dc.description.abstract [EN] Wounded leaves of Arabidopsis thaliana show transient immunity to Botrytis cinerea, the causal agent of grey mould. Using a fluorescent probe, histological staining and a luminol assay, we now show that reactive oxygen species (ROS), including H 2O 2 and O 2 -, are produced within minutes after wounding. ROS are formed in the absence of the enzymes Atrboh D and F and can be prevented by diphenylene iodonium (DPI) or catalase. H 2O 2 was shown to protect plants upon exogenous application. ROS accumulation and resistance to B. cinerea were abolished when wounded leaves were incubated under dry conditions, an effect that was found to depend on abscisic acid (ABA). Accordingly, ABA biosynthesis mutants (aba2 and aba3) were still fully resistant under dry conditions even without wounding. Under dry conditions, wounded plants contained higher ABA levels and displayed enhanced expression of ABA-dependent and ABA-reporter genes. Mutants impaired in cutin synthesis such as bdg and lacs2.3 are already known to display a high level of resistance to B. cinerea and were found to produce ROS even when leaves were not wounded. An increased permeability of the cuticle and enhanced ROS production were detected in aba2 and aba3 mutants as described for bdg and lacs2.3. Moreover, leaf surfaces treated with cutinase produced ROS and became more protected to B. cinerea. Thus, increased permeability of the cuticle is strongly linked with ROS formation and resistance to B. cinerea. The amount of oxalic acid, an inhibitor of ROS secreted by B. cinerea could be reduced using plants over expressing a fungal oxalate decarboxylase of Trametes versicolor. Infection of such plants resulted in a faster ROS accumulation and resistance to B. cinerea than that observed in untransformed controls, demonstrating the importance of fungal suppression of ROS formation by oxalic acid. Thus, changes in the diffusive properties of the cuticle are linked with the induction ROS and attending innate defenses. © 2011 L'Haridon et al. es_ES
dc.description.sponsorship This work was made possible by funds to JPM from the Swiss National Science Foundation (grant 125370). Jose Leon was supported by Ministerio de Ciencia e Innovacion of Spain (BIO2008-00839) and Ricardo Mir by a FPU Fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS Pathogens es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Abscisic acid es_ES
dc.subject Abscisic acid 2 es_ES
dc.subject Abscisic acid 3 es_ES
dc.subject Atrboh D es_ES
dc.subject Atrboh F es_ES
dc.subject Bdg protein es_ES
dc.subject Carboxylyase es_ES
dc.subject Catalase es_ES
dc.subject Cutinase es_ES
dc.subject Diphenyliodonium salt es_ES
dc.subject Enzyme es_ES
dc.subject Fluorescent dye es_ES
dc.subject Hydrogen peroxide es_ES
dc.subject Lacs2.3 protein es_ES
dc.subject Luminol es_ES
dc.subject Oxalate decarboxylase es_ES
dc.subject Oxalic acid es_ES
dc.subject Oxygen es_ES
dc.subject Phytohormone es_ES
dc.subject Reactive oxygen metabolite es_ES
dc.subject Unclassified drug es_ES
dc.subject Vegetable protein es_ES
dc.subject Arabidopsis protein es_ES
dc.subject BDG protein, Arabidopsis es_ES
dc.subject Cutine es_ES
dc.subject Fungal protein es_ES
dc.subject LACS2 protein, Arabidopsis es_ES
dc.subject Long chain fatty acid coenzyme A ligase es_ES
dc.subject Membrane lipid es_ES
dc.subject Superoxide es_ES
dc.subject Animal experiment es_ES
dc.subject Article es_ES
dc.subject Biosynthesis es_ES
dc.subject Botrytis cinerea es_ES
dc.subject Cell permeabilization es_ES
dc.subject Controlled study es_ES
dc.subject Cuticle es_ES
dc.subject Dry deposition es_ES
dc.subject Gene expression es_ES
dc.subject Hormone synthesis es_ES
dc.subject Immunoassay es_ES
dc.subject Innate immunity es_ES
dc.subject Mutant es_ES
dc.subject Nonhuman es_ES
dc.subject Plant disease es_ES
dc.subject Plant leaf es_ES
dc.subject Protein function es_ES
dc.subject Protein secretion es_ES
dc.subject Protein synthesis es_ES
dc.subject Reporter gene es_ES
dc.subject Trametes versicolor es_ES
dc.subject Wound es_ES
dc.subject Arabidopsis es_ES
dc.subject Botrytis es_ES
dc.subject Genetics es_ES
dc.subject Immunology es_ES
dc.subject Metabolism es_ES
dc.subject Microbiology es_ES
dc.subject Mutation es_ES
dc.subject Physiology es_ES
dc.subject Plant immunity es_ES
dc.subject Trametes es_ES
dc.subject Transgenic plant es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject Botryotinia fuckeliana es_ES
dc.subject Arabidopsis Proteins es_ES
dc.subject Carboxy-Lyases es_ES
dc.subject Coenzyme A Ligases es_ES
dc.subject Fungal Proteins es_ES
dc.subject Membrane Lipids es_ES
dc.subject Plant Diseases es_ES
dc.subject Plant Leaves es_ES
dc.subject Plants, Genetically Modified es_ES
dc.subject Superoxides es_ES
dc.title A permeable cuticle is associated with the release of reactive oxygen species and induction of innate immunity es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.ppat.1002148
dc.relation.projectID info:eu-repo/grantAgreement/SNSF//25370/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/SNSF/Project funding/31003A_125370/CH/ en_EN
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2008-00839/ES/BIOSINTESIS Y FUNCION DEL OXIDO NITRICO EN ARABIDOPSIS. CONEXION CON LOS ACIDOS ABSCISICO, SALICILICO Y JASMONICO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation L'haridon, F.; Besson-Bard, A.; Binda, M.; Serrano Mateo, M.; Abou-Mansour, E.; Balet, F.; Schoonbeek, H.... (2011). A permeable cuticle is associated with the release of reactive oxygen species and induction of innate immunity. PLoS Pathogens. 7:1002148-1002148. https://doi.org/10.1371/journal.ppat.1002148 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1371/journal.ppat.1002148 es_ES
dc.description.upvformatpinicio 1002148 es_ES
dc.description.upvformatpfin 1002148 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.relation.senia 213961
dc.identifier.pmid 21829351 en_EN
dc.identifier.pmcid PMC3145797
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Swiss National Science Foundation
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