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Arrangement of Type IV Collagen and Laminin on Substrates with Controlled Density of ¿OH Groups

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Arrangement of Type IV Collagen and Laminin on Substrates with Controlled Density of ¿OH Groups

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dc.contributor.author Coelho, Nuno Miranda es_ES
dc.contributor.author González García, Cristina es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.contributor.author Altankov, George
dc.date.accessioned 2013-06-26T06:50:03Z
dc.date.available 2013-06-26T06:50:03Z
dc.date.issued 2011
dc.identifier.issn 1937-3341
dc.identifier.uri http://hdl.handle.net/10251/30116
dc.description This is a copy of an article published in the Diabetes Technology and Therapeutics © 2011 [copyright Mary Ann Liebert, Inc.]; Diabetes Technology and Therapeutics is available online at: http://online.liebertpub.com.
dc.description.abstract Collagen IV (Col IV) and laminin (Lam) are the main structural components of the basement membrane where they form two overlapping polymeric networks. We studied the adsorption pattern of these proteins on five model surfaces with tailored density of-OH groups obtained by copolymerization of different ratios ethyl acrylate (EA) and hydroxyl EA (HEA): X OH=0, X OH=0.3, X OH=0.5, X OH=0.7, and X OH=1 (where X refers the ratio of HEA). Atomic force microscopy revealed substratum-specific adsorption patterns of Col IV and Lam, ranging from single molecules deposition on more hydrophilic substrata to the formation of complex networks on hydrophobic ones. Human umbilical endothelial cells were used to study the biological performance of adsorbed proteins, following the overall cell morphology, the quantities for cell adhesion and spreading, and the development of focal adhesion complexes and actin cytoskeleton. Surprisingly, two optima in the cellular interaction were observed-one on the most hydrophilic X OH=1 and other on the relatively hydrophobic X OH=0.3 substrate-valid for both Col IV and Lam. When the proteins were adsorbed consecutively, a hydrophobic shift to X OH=0 substratum was obtained. Collectively, these data suggest that varying with the density of-OH groups one can tailor the conformation and the functional activity of adsorbed basement membrane proteins. © 2011, Mary Ann Liebert, Inc. es_ES
dc.description.sponsorship AFM was performed under the technical guidance of the Microscopy Service at the Universidad Politecnica de Valencia, whose advice is greatly appreciated. The work was supported by the Spanish Ministry of Science and Innovation through project nos. MAT2009-14440-C02-0 and MAT2009-14440-C02-02. en_EN
dc.language Inglés es_ES
dc.publisher Mary Ann Liebert es_ES
dc.relation Spanish Ministry of Science and Innovation MAT2009-14440-C02-0 MAT2009-14440-C02-02 es_ES
dc.relation.ispartof Tissue Engineering Part A es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Actin cytoskeleton es_ES
dc.subject Adsorption patterns es_ES
dc.subject Basement membrane es_ES
dc.subject Biological performance es_ES
dc.subject Cell morphology es_ES
dc.subject Cellular interaction es_ES
dc.subject Complex networks es_ES
dc.subject Ethyl acrylates es_ES
dc.subject Focal adhesions es_ES
dc.subject Functional activities es_ES
dc.subject Human umbilical endothelial cells es_ES
dc.subject Laminin es_ES
dc.subject Model surface es_ES
dc.subject Polymeric networks es_ES
dc.subject Single molecule es_ES
dc.subject Structural component es_ES
dc.subject Adsorption es_ES
dc.subject Atomic force microscopy es_ES
dc.subject Biological membranes es_ES
dc.subject Buildings es_ES
dc.subject Cell adhesion es_ES
dc.subject Collagen es_ES
dc.subject Endothelial cells es_ES
dc.subject Hydrophilicity es_ES
dc.subject Hydrophobicity es_ES
dc.subject Substrates es_ES
dc.subject Adhesion es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Arrangement of Type IV Collagen and Laminin on Substrates with Controlled Density of ¿OH Groups es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1089/ten.tea.2010.0713
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Coelho, NM.; González García, C.; Salmerón Sánchez, M.; Altankov, G. (2011). Arrangement of Type IV Collagen and Laminin on Substrates with Controlled Density of ¿OH Groups. Tissue Engineering Part A. 17:2245-2257. doi:10.1089/ten.tea.2010.0713 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1089/ten.tea.2010.0713 es_ES
dc.description.upvformatpinicio 2245 es_ES
dc.description.upvformatpfin 2257 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 17 es_ES
dc.relation.senia 219971


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