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Carbon nanoparticles as detection labels in antibody microarrays.Detection of genes encoding virulence factors in Shiga toxin-producing Escherichia coli

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Carbon nanoparticles as detection labels in antibody microarrays.Detection of genes encoding virulence factors in Shiga toxin-producing Escherichia coli

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dc.contributor.author Noguera Murray, Patricia Silvestre es_ES
dc.contributor.author Posthuma-Trumpie, Geertruida A es_ES
dc.contributor.author van Tuil, Marc es_ES
dc.contributor.author van der Wal, Fimme J. es_ES
dc.contributor.author de Boer, Albert es_ES
dc.contributor.author Moers, Antoine P. H. A. es_ES
dc.contributor.author van Amerongen, Aart es_ES
dc.date.accessioned 2013-06-26T06:58:03Z
dc.date.issued 2011-09-21
dc.identifier.issn 0003-2700
dc.identifier.uri http://hdl.handle.net/10251/30120
dc.description.abstract The present study demonstrates that carbon nanoparticles (CNPs) can be used as labels in microarrays. CNPs were used in nucleic acid microarray immunoassays (NAMIAs) for the detection of di¿erent Shiga toxin-producing Escherichia coli (STEC) virulence factors: four genes speci¿c for STEC (vt1,vt2, eae, andehxA) and the gene forE. coli16S (hui). Optimization was performed using a Box Behnken design, andthe limit of detection for each virulence factor was established. Finally,this NAMIA using CNPs was tested with DNA from 48 ¿eld strains originating from cattle feces, and its performance was evaluated by comparing results with those achieved by the reference method q-PCR. All factors tested gave sensitivity and speci¿city values higher than 0.80 and e¿ciency values higher than 0.92. Kappa coe¿cients showed an almost perfect agreement (k > 0.8) between NAMIA and the reference method used forvt1, eae, and ehxA, and a perfect agreement (k = 1) forvt2 and hui. The excellent agreement between the developed NAMIA and q-PCR demonstrates that the proposed analytical procedure is indeed ¿t for purpose, i.e., it is valuable for fast screening of ampli¿ed genetic material such as E. coli virulence factors. This also proves the applicability of CNPs in microarrays. es_ES
dc.description.sponsorship This work was partially supported by the Generalitat Valenciana (BEST/2009/026), the Universidad Politecnica de Valencia (PAID-00-09-2837), and the Dutch Ministry of Agriculture, Nature and Food Quality (Strategic research program Food Safety, Monitoring and Detection KB-06-005). The authors thank Dr. Eva Moller Nielsen at the Danish Veterinary Institute (Copenhagen, Denmark) for providing E. coli control strains and Dr. Lutz Geue (Friedrich-Loeffler-Institut, Wusterhausen, Germany) and Dr. Dorte Dopfer (School of Veterinary Medicine, University of Wisconsin, Madison, WI) for field isolates. en_EN
dc.language Inglés es_ES
dc.publisher American Chemical Society es_ES
dc.relation info:eu-repo/grantAgreement/Generalitat Valenciana//BEST%2F2009%2F026/ES/BEST%2F2009%2F026/ es_ES
dc.relation Universidad Politecnica de Valencia PAID-00-09-2837 es_ES
dc.relation Dutch Ministry of Agriculture, Nature and Food Quality KB-06-005 es_ES
dc.relation.ispartof Analytical Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Pathogen detection es_ES
dc.subject DNA microarray es_ES
dc.subject Bacteria es_ES
dc.subject Assay es_ES
dc.subject Inmunoassay es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Carbon nanoparticles as detection labels in antibody microarrays.Detection of genes encoding virulence factors in Shiga toxin-producing Escherichia coli es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1021/ac201823v
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Noguera Murray, PS.; Posthuma-Trumpie, GA.; Van Tuil, M.; Van Der Wal, FJ.; De Boer, A.; Moers, APHA.; Van Amerongen, A. (2011). Carbon nanoparticles as detection labels in antibody microarrays.Detection of genes encoding virulence factors in Shiga toxin-producing Escherichia coli. Analytical Chemistry. 83:8531-8536. https://doi.org/10.1021/ac201823v es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://pubs.acs.org/doi/ipdf/10.1021/ac201823v es_ES
dc.description.upvformatpinicio 8531 es_ES
dc.description.upvformatpfin 8536 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 83 es_ES
dc.relation.senia 211041
dc.contributor.funder Generalitat Valenciana es_ES


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