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Involvement of nitric oxide (NO) and auxin in signal transduction of copper induced morphological responses in Arabidopsis seedlings.

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Involvement of nitric oxide (NO) and auxin in signal transduction of copper induced morphological responses in Arabidopsis seedlings.

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dc.contributor.author Petó, Andrea es_ES
dc.contributor.author Lehotai, Nóra es_ES
dc.contributor.author Lozano Juste, Jorge es_ES
dc.contributor.author Leon Ramos, Jose es_ES
dc.contributor.author Tari, Irma es_ES
dc.contributor.author Erdei, László es_ES
dc.contributor.author Kolbert, Zsuzsanna es_ES
dc.date.accessioned 2013-06-27T10:13:22Z
dc.date.issued 2011-05-19
dc.identifier.issn 0305-7364
dc.identifier.uri http://hdl.handle.net/10251/30234
dc.description.abstract Background and Aims Plants are able to adapt to the environment dynamically through regulation of their growth and development. Excess copper (Cu2+ ), a toxic heavy metal, induces morphological alterations in plant organs; however, the underlying mechanisms are still unclear. With this in mind, the multiple signalling functions of nitric oxide (NO) in plant cells and its possible regulatory role and relationship with auxin were examined during Cu2+ -induced morphological responses. Methods Endogenous auxin distribution was determined by microscopic observation of X-Gluc-stained DR5::GUS arabidopsis, and the levels of NO, superoxide and peroxynitrite were detected by ¿uorescence microscopy. As well as wild-type, NO-overproducer (nox1) and -de¿cient (nia1nia2 and nia1nia2noa1-2) arabidopsis plants were used. Key Results Cu2+ at a concentration of 50mM resulted in a large reduction in cotyledon area and hypocotyl and primary root lengths, accompanied by an increase in auxin levels. In cotyledons, a low Cu2+ concentration promoted NO accumulation, which was arrested by nitric oxide synthase or nitrate reductase inhibitors. The 5-mM Cu2+ -induced NO synthesis was not detectable in nia1nia2 or nia1nia2noa1-2 plants. In roots, Cu2+ caused a decrease of the NO level which was not associated with superoxide and peroxynitrite formation. Inhibition of auxin transport resulted in an increase in NO levels, while exogenous application of an NO donor reduced DR5::GUS expression. The elongation processes of nox1 were not sensitive to Cu2+ , but NO-de¿cient plants showed diverse growth responses. ConclusionsIn plant organs, Cu2+ excess results in severe morphological responses during which the endogenous hormonal balance and signal transduction are affected. Auxin and NO negatively regulate each other¿s level and NO intensi¿es the metal-induced cotyledon expansion, but mitigates elongation processes under Cu2+ exposure es_ES
dc.language Inglés es_ES
dc.publisher Oxford University Press (OUP): Policy B - Oxford Open Option A es_ES
dc.relation.ispartof Annals of Botany es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject Auxin es_ES
dc.subject Copper es_ES
dc.subject Morphological responses es_ES
dc.subject Nitric oxide es_ES
dc.title Involvement of nitric oxide (NO) and auxin in signal transduction of copper induced morphological responses in Arabidopsis seedlings. es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1093/aob/mcr176
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Petó, A.; Lehotai, N.; Lozano Juste, J.; Leon Ramos, J.; Tari, I.; Erdei, L.; Kolbert, Z. (2011). Involvement of nitric oxide (NO) and auxin in signal transduction of copper induced morphological responses in Arabidopsis seedlings. Annals of Botany. 108(3):449-457. doi:10.1093/aob/mcr176 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://aob.oxfordjournals.org/content/108/3/449.full.pdf+html es_ES
dc.description.upvformatpinicio 449 es_ES
dc.description.upvformatpfin 457 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 108 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 213980


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