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Fruit load modulates flowering-related gene expression in buds of alternate-bearing 'Moncada' mandarin

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Fruit load modulates flowering-related gene expression in buds of alternate-bearing 'Moncada' mandarin

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dc.contributor.author Muñoz Fambuena, Natalia es_ES
dc.contributor.author Mesejo Conejos, Carlos es_ES
dc.contributor.author Gonzalez Más, Mª Carmen es_ES
dc.contributor.author Primo-Millo, Eduardo es_ES
dc.contributor.author Agustí Fonfría, Manuel es_ES
dc.contributor.author Iglesias, Domingo J. es_ES
dc.date.accessioned 2013-06-27T11:33:03Z
dc.date.issued 2012-08-21
dc.identifier.issn 0305-7364
dc.identifier.uri http://hdl.handle.net/10251/30237
dc.description.abstract Background and Aims Gene determination of flowering is the result of complex interactions involving both promoters and inhibitors. In this study, the expression of flowering-related genes at the meristem level in alternate-bearing citrus trees is analysed, together with the interplay between buds and leaves in the determination of flowering. Methods First defruiting experiments were performed to manipulate blossoming intensity in `Moncada¿ mandarin, Citrus clementina. Further defoliation was performed to elucidate the role leaves play in the flowering process. In both cases, the activity of flowering-related genes was investigated at the flower induction (November) and differentiation (February) stages. Key Results Study of the expression pattern of flowering-genes in buds from on (fully loaded) and off (without fruits) trees revealed that homologues of FLOWERING LOCUS T (CiFT), TWIN SISTER OF FT (TSF), APETALA1 (CsAP1) and LEAFY (CsLFY) were negatively affected by fruit load. CiFT and TSF activities showed a marked increase in buds from off trees through the study period (ten-fold in November). By contrast, expression of the homologues of the flowering inhibitors of TERMINAL FLOWER 1 (CsTFL), TERMINAL FLOWER 2 (TFL2) and FLOWERING LOCUS C (FLC) was generally lower in off trees. Regarding floral identity genes, the increase in CsAP1 expression in off trees was much greater in buds than in leaves, and significant variations in CsLFY expression (approx. 20 %) were found only in February. Defoliation experiments further revealed that the absence of leaves completely abolished blossoming and severely affected the expression of most of the flowering-related genes, particularly decreasing the activity of floral promoters and of CsAP1 at the induction stage. Conclusions These results suggest that the presence of fruit affects flowering by greatly altering gene-expression not only at the leaf but also at the meristem level. Although leaves are required for flowering to occur, their absence strongly affects the activity of floral promoters and identity genes. es_ES
dc.description.sponsorship This work was supported by a grant from the Instituto Nacional Investigaciones Agrarias, Spain (RTA2009-00147). M. C. Gonzalez was the recipient of a contract by the Fundacion Agroalimed (Conselleria d'Agricultura, Pesca i Alimentacio, Generalitat Valenciana). en_EN
dc.language Inglés es_ES
dc.publisher Oxford University Press (OUP): Policy B - Oxford Open Option A es_ES
dc.relation.ispartof Annals of Botany es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Alternate bearing es_ES
dc.subject Citrus es_ES
dc.subject Flowering es_ES
dc.subject Orange citrus-sinensis es_ES
dc.subject Trifoliata L. Raf es_ES
dc.subject Floral induction es_ES
dc.subject Meristem identity es_ES
dc.subject Satsuma mandarin es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.title Fruit load modulates flowering-related gene expression in buds of alternate-bearing 'Moncada' mandarin es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1093/aob/mcs190
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RTA2009-00147/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.description.bibliographicCitation Muñoz Fambuena, N.; Mesejo Conejos, C.; Gonzalez Más, MC.; Primo-Millo, E.; Agustí Fonfría, M.; Iglesias, DJ. (2012). Fruit load modulates flowering-related gene expression in buds of alternate-bearing 'Moncada' mandarin. Annals of Botany. 110(6):1109-1118. https://doi.org/10.1093/aob/mcs190 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://aob.oxfordjournals.org/content/110/6/1109.full.pdf+html es_ES
dc.description.upvformatpinicio 1109 es_ES
dc.description.upvformatpfin 1118 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 110 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 237530
dc.identifier.pmid 22915579 en_EN
dc.identifier.pmcid PMC3478051
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Fundación de la Comunitat Valenciana para la Investigación Agroalimentaria, Agroalimed es_ES
dc.description.references Abe, M. (2005). FD, a bZIP Protein Mediating Signals from the Floral Pathway Integrator FT at the Shoot Apex. Science, 309(5737), 1052-1056. doi:10.1126/science.1115983 es_ES
dc.description.references Bustin, S. (2002). Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. Journal of Molecular Endocrinology, 23-39. doi:10.1677/jme.0.0290023 es_ES
dc.description.references Corbesier, L., & Coupland, G. (2006). The quest for florigen: a review of recent progress. Journal of Experimental Botany, 57(13), 3395-3403. doi:10.1093/jxb/erl095 es_ES
dc.description.references Dornelas, M. C., Camargo, R. L. B., Figueiredo, L. H. M., & Takita, M. A. (2007). A genetic framework for flowering-time pathways in Citrus spp. Genetics and Molecular Biology, 30(3 suppl), 769-779. doi:10.1590/s1415-47572007000500006 es_ES
dc.description.references Endo, T., Shimada, T., Fujii, H., Kobayashi, Y., Araki, T., & Omura, M. (2005). Ectopic Expression of an FT Homolog from Citrus Confers an Early Flowering Phenotype on Trifoliate Orange (Poncirus trifoliata L. Raf.). Transgenic Research, 14(5), 703-712. doi:10.1007/s11248-005-6632-3 es_ES
dc.description.references Esumi, T., Hagihara, C., Kitamura, Y., Yamane, H., & Tao, R. (2009). Identification of anFTortholog in Japanese apricot (Prunus mumeSieb. et Zucc.). The Journal of Horticultural Science and Biotechnology, 84(2), 149-154. doi:10.1080/14620316.2009.11512496 es_ES
dc.description.references Esumi, T., Kitamura, Y., Hagihara, C., Yamane, H., & Tao, R. (2010). Identification of a TFL1 ortholog in Japanese apricot (Prunus mume Sieb. et Zucc.). Scientia Horticulturae, 125(4), 608-616. doi:10.1016/j.scienta.2010.05.016 es_ES
dc.description.references Giakountis, A., & Coupland, G. (2008). Phloem transport of flowering signals. Current Opinion in Plant Biology, 11(6), 687-694. doi:10.1016/j.pbi.2008.10.003 es_ES
dc.description.references Hashimoto, J. G., Beadles-Bohling, A. S., & Wiren, K. M. (2004). Comparison of RiboGreen®and 18S rRNA quantitation for normalizing real-time RT-PCR expression analysis. BioTechniques, 36(1), 54-60. doi:10.2144/04361bm06 es_ES
dc.description.references Jaeger, K. E., Graf, A., & Wigge, P. A. (2006). The control of flowering in time and space. Journal of Experimental Botany, 57(13), 3415-3418. doi:10.1093/jxb/erl159 es_ES
dc.description.references Jang, S., Torti, S., & Coupland, G. (2009). Genetic and spatial interactions betweenFT,TSFandSVPduring the early stages of floral induction in Arabidopsis. The Plant Journal, 60(4), 614-625. doi:10.1111/j.1365-313x.2009.03986.x es_ES
dc.description.references Jaya, E. S. K. D., Clemens, J., Song, J., Zhang, H., & Jameson, P. E. (2009). Quantitative expression analysis of meristem identity genes in Eucalyptus occidentalis: AP1 is an expression marker for flowering. Tree Physiology, 30(2), 304-312. doi:10.1093/treephys/tpp117 es_ES
dc.description.references Koshita, Y., Takahara, T., Ogata, T., & Goto, A. (1999). Involvement of endogenous plant hormones (IAA, ABA, GAs) in leaves and flower bud formation of satsuma mandarin (Citrus unshiu Marc.). Scientia Horticulturae, 79(3-4), 185-194. doi:10.1016/s0304-4238(98)00209-x es_ES
dc.description.references Kotoda, N., Hayashi, H., Suzuki, M., Igarashi, M., Hatsuyama, Y., Kidou, S., … Abe, K. (2010). Molecular Characterization of FLOWERING LOCUS T-Like Genes of Apple (Malus × domestica Borkh.). Plant and Cell Physiology, 51(4), 561-575. doi:10.1093/pcp/pcq021 es_ES
dc.description.references Li, D., Liu, C., Shen, L., Wu, Y., Chen, H., Robertson, M., … Yu, H. (2008). A Repressor Complex Governs the Integration of Flowering Signals in Arabidopsis. Developmental Cell, 15(1), 110-120. doi:10.1016/j.devcel.2008.05.002 es_ES
dc.description.references Lord, E. M., & Eckard, K. J. (1985). Shoot Development in Citrus sinensis L. (Washington Navel Orange). I. Floral and Inflorescence Ontogeny. Botanical Gazette, 146(3), 320-326. doi:10.1086/337531 es_ES
dc.description.references Mathieu, J., Warthmann, N., Küttner, F., & Schmid, M. (2007). Export of FT Protein from Phloem Companion Cells Is Sufficient for Floral Induction in Arabidopsis. Current Biology, 17(12), 1055-1060. doi:10.1016/j.cub.2007.05.009 es_ES
dc.description.references Matsuda, N., Ikeda, K., Kurosaka, M., Takashina, T., Isuzugawa, K., Endo, T., & Omura, M. (2009). Early Flowering Phenotype in Transgenic Pears (Pyrus communis L.) Expressing the CiFT Gene. Journal of the Japanese Society for Horticultural Science, 78(4), 410-416. doi:10.2503/jjshs1.78.410 es_ES
dc.description.references Michaels, S. D., Himelblau, E., Kim, S. Y., Schomburg, F. M., & Amasino, R. M. (2004). Integration of Flowering Signals in Winter-Annual Arabidopsis. Plant Physiology, 137(1), 149-156. doi:10.1104/pp.104.052811 es_ES
dc.description.references Moss, G. I. (1971). Effect of fruit on flowering in relation to biennial bearing in sweet orange(Citrus sinensis). Journal of Horticultural Science, 46(2), 177-184. doi:10.1080/00221589.1971.11514396 es_ES
dc.description.references Muñoz-Fambuena, N., Mesejo, C., Carmen González-Mas, M., Primo-Millo, E., Agustí, M., & Iglesias, D. J. (2011). Fruit regulates seasonal expression of flowering genes in alternate-bearing ‘Moncada’ mandarin. Annals of Botany, 108(3), 511-519. doi:10.1093/aob/mcr164 es_ES
dc.description.references Muñoz-Fambuena, N., Mesejo, C., González-Mas, M. C., Iglesias, D. J., Primo-Millo, E., & Agustí, M. (2012). Gibberellic Acid Reduces Flowering Intensity in Sweet Orange [Citrus sinensis (L.) Osbeck] by Repressing CiFT Gene Expression. Journal of Plant Growth Regulation, 31(4), 529-536. doi:10.1007/s00344-012-9263-y es_ES
dc.description.references Nishikawa, F., Endo, T., Shimada, T., Fujii, H., Shimizu, T., Omura, M., & Ikoma, Y. (2007). Increased CiFT abundance in the stem correlates with floral induction by low temperature in Satsuma mandarin (Citrus unshiu Marc.). Journal of Experimental Botany, 58(14), 3915-3927. doi:10.1093/jxb/erm246 es_ES
dc.description.references Nishikawa, F., Endo, T., Shimada, T., Fujii, H., Shimizu, T., Kobayashi, Y., … Omura, M. (2010). Transcriptional changes in CiFT-introduced transgenic trifoliate orange (Poncirus trifoliata L. Raf.). Tree Physiology, 30(3), 431-439. doi:10.1093/treephys/tpp122 es_ES
dc.description.references Notaguchi, M., Abe, M., Kimura, T., Daimon, Y., Kobayashi, T., Yamaguchi, A., … Araki, T. (2008). Long-Distance, Graft-Transmissible Action of Arabidopsis FLOWERING LOCUS T Protein to Promote Flowering. Plant and Cell Physiology, 49(11), 1645-1658. doi:10.1093/pcp/pcn154 es_ES
dc.description.references Peña, L., Martín-Trillo, M., Juárez, J., Pina, J. A., Navarro, L., & Martínez-Zapater, J. M. (2001). Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time. Nature Biotechnology, 19(3), 263-267. doi:10.1038/85719 es_ES
dc.description.references Pillitteri, L. J., Lovatt, C. J., & Walling, L. L. (2004). Isolation and Characterization of a TERMINAL FLOWER Homolog and Its Correlation with Juvenility in Citrus. Plant Physiology, 135(3), 1540-1551. doi:10.1104/pp.103.036178 es_ES
dc.description.references Pillitteri, L. J., Lovatt, C. J., & Walling, L. L. (2004). Isolation and Characterization of LEAFY and APETALA1 Homologues from Citrus sinensis L. Osbeck `Washington’. Journal of the American Society for Horticultural Science, 129(6), 846-856. doi:10.21273/jashs.129.6.0846 es_ES
dc.description.references Rottmann, W. H., Meilan, R., Sheppard, L. A., Brunner, A. M., Skinner, J. S., Ma, C., … Strauss, S. H. (2000). Diverse effects of overexpression of LEAFY and PTLF, a poplar (Populus) homolog of LEAFY/FLORICAULA, in transgenic poplar and Arabidopsis. The Plant Journal, 22(3), 235-245. doi:10.1046/j.1365-313x.2000.00734.x es_ES
dc.description.references Sherman, W. B., & Beckman, T. G. (2003). CLIMATIC ADAPTATION IN FRUIT CROPS. Acta Horticulturae, (622), 411-428. doi:10.17660/actahortic.2003.622.43 es_ES
dc.description.references Southerton, S. G., Strauss, S. H., Olive, M. R., Harcourt, R. L., Decroocq, V., Zhu, X., … Dennis, E. S. (1998). Plant Molecular Biology, 37(6), 897-910. doi:10.1023/a:1006056014079 es_ES
dc.description.references Sreekantan, L., & Thomas, M. R. (2006). VvFT and VvMADS8, the grapevine homologues of the floral integrators FT and SOC1, have unique expression patterns in grapevine and hasten flowering in Arabidopsis. Functional Plant Biology, 33(12), 1129. doi:10.1071/fp06144 es_ES
dc.description.references Takada, S., & Goto, K. (2003). TERMINAL FLOWER2, an Arabidopsis Homolog of HETEROCHROMATIN PROTEIN1, Counteracts the Activation of FLOWERING LOCUS T by CONSTANS in the Vascular Tissues of Leaves to Regulate Flowering Time. The Plant Cell, 15(12), 2856-2865. doi:10.1105/tpc.016345 es_ES
dc.description.references Tan, F.-C., & Swain, S. M. (2007). Functional characterization of AP3, SOC1 and WUS homologues from citrus (Citrus sinensis). Physiologia Plantarum, 131(3), 481-495. doi:10.1111/j.1399-3054.2007.00971.x es_ES
dc.description.references Tränkner, C., Lehmann, S., Hoenicka, H., Hanke, M.-V., Fladung, M., Lenhardt, D., … Flachowsky, H. (2010). Over-expression of an FT-homologous gene of apple induces early flowering in annual and perennial plants. Planta, 232(6), 1309-1324. doi:10.1007/s00425-010-1254-2 es_ES
dc.description.references Vemmos, S. N. (1999). Carbohydrate content of inflorescent buds of defruited and fruiting pistachio (Pistacia vera L) branches in relation to biennial bearing. The Journal of Horticultural Science and Biotechnology, 74(1), 94-100. doi:10.1080/14620316.1999.11511079 es_ES
dc.description.references Wada, M., Cao, Q., Kotoda, N., Soejima, J., & Masuda, T. (2002). Plant Molecular Biology, 49(6), 567-577. doi:10.1023/a:1015544207121 es_ES
dc.description.references Wigge, P. A. (2005). Integration of Spatial and Temporal Information During Floral Induction in Arabidopsis. Science, 309(5737), 1056-1059. doi:10.1126/science.1114358 es_ES
dc.description.references Yahata, D., Matsumoto, K., & Ushijima, K. (2004). Relationship between Flower-bud Differentiation and Carbohydrate Contents in Spring Shoots of Very-early, Early and Late Maturing Cultivars of Satsuma Mandarin. Engei Gakkai zasshi, 73(5), 405-410. doi:10.2503/jjshs.73.405 es_ES
dc.description.references Yamaguchi, A., Kobayashi, Y., Goto, K., Abe, M., & Araki, T. (2005). TWIN SISTER OF FT (TSF) Acts as a Floral Pathway Integrator Redundantly with FT. Plant and Cell Physiology, 46(8), 1175-1189. doi:10.1093/pcp/pci151 es_ES
dc.description.references Yan, J., Yuan, F., Long, G., Qin, L., & Deng, Z. (2011). Selection of reference genes for quantitative real-time RT-PCR analysis in citrus. Molecular Biology Reports, 39(2), 1831-1838. doi:10.1007/s11033-011-0925-9 es_ES
dc.description.references YU, Q., MOORE, P. H., ALBERT, H. H., ROADER, A. H. K., & MING, R. (2005). Cloning and characterization of a FLORICAULA/LEAFY ortholog, PFL, in polygamous papaya. Cell Research, 15(8), 576-584. doi:10.1038/sj.cr.7290327 es_ES
dc.description.references Yu, X., Klejnot, J., & Lin, C. (2006). Florigen: One Found, More to Follow? Journal of Integrative Plant Biology, 48(6), 617-621. doi:10.1111/j.1744-7909.2006.00309.x es_ES


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