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dc.contributor.author | Estevez, M. Carmen | es_ES |
dc.contributor.author | Belenguer Ballester, José | es_ES |
dc.contributor.author | Gómez Montes, Silvia | es_ES |
dc.contributor.author | Miralles Garcia, Javier | es_ES |
dc.contributor.author | Escuela, Alfonso M. | es_ES |
dc.contributor.author | Montoya Baides, Ángel | es_ES |
dc.contributor.author | Lechuga, Laura M. | es_ES |
dc.date.accessioned | 2013-07-08T11:49:15Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0003-2654 | |
dc.identifier.uri | http://hdl.handle.net/10251/30822 | |
dc.description.abstract | [EN] A highly sensitive and speci¿c SPR-based competitive immunoassay for the detection of Thiabendazole (TBZ) has been developed. An indirect format where a TBZ¿protein conjugate is immobilized onto gold surfaces has been selected. Under the optimal conditions, a LOD of 0.67 nM (0.13 mg L 1 ) and an IC50 of 3.2 nM (0.64 mg L 1 ) have been achieved which are comparable to the values obtained by conventional ELISA. Analysis of real samples has been attempted by ¿rst evaluating the in¿uence of complex matrix samples coming from whole oranges and secondly measuring samples containing TBZ previously evaluated by chromatographic methods. A methanolic extraction procedure followed by a simple dilution in assay buffer has proven to be suf¿cient to measure orange samples using the developed immunoassay with an excellent recovery percentage. The sensitivity and the feasibility of measuring whole orange samples demonstrate the effectiveness and robustness of the SPR biosensor, which can be useful for the determination of TBZ in food at concentrations below the Maximum Residue Levels (MRLs) established by the European legislation. | es_ES |
dc.description.sponsorship | MCE, SG and LML acknowledge the financial support from Fundacion M. Botin. JB thanks Instituto de la Mediana y Pequena Industria Valenciana (IMPIVA) under the activity "Proyectos de alta especializacion del personal investigador''. | |
dc.language | Inglés | es_ES |
dc.publisher | Royal Society of Chemistry | es_ES |
dc.relation.ispartof | Analyst | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject.classification | TECNOLOGIA ELECTRONICA | es_ES |
dc.title | Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance | es_ES |
dc.type | Artículo | es_ES |
dc.embargo.lift | 10000-01-01 | |
dc.embargo.terms | forever | es_ES |
dc.identifier.doi | 10.1039/C2AN36094B | |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano - Institut Interuniversitari d'Investigació en Bioenginyeria i Tecnologia Orientada a l'Ésser Humà | es_ES |
dc.description.bibliographicCitation | Estevez, MC.; Belenguer Ballester, J.; Gómez Montes, S.; Miralles Garcia, J.; Escuela, AM.; Montoya Baides, Á.; Lechuga, LM. (2012). Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance. Analyst. 137(23):5659-5665. doi:10.1039/C2AN36094B | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1039/C2AN36094B | es_ES |
dc.description.upvformatpinicio | 5659 | es_ES |
dc.description.upvformatpfin | 5665 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 137 | es_ES |
dc.description.issue | 23 | es_ES |
dc.relation.senia | 240723 | |
dc.identifier.eissn | 1364-5528 | |
dc.identifier.pmid | 23057069 | |
dc.contributor.funder | Fundación Botín | |
dc.contributor.funder | Instituto de la Pequeña y Mediana Industria de la Generalitat Valenciana |