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Chemical silicon surface modification and bioreceptor attachment to develop competitive integrated photonic biosensors

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Chemical silicon surface modification and bioreceptor attachment to develop competitive integrated photonic biosensors

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dc.contributor.author Escorihuela Fuentes, Jorge es_ES
dc.contributor.author Bañuls Polo, Mª José es_ES
dc.contributor.author García Castelló, Javier es_ES
dc.contributor.author Toccafondo, Veronica es_ES
dc.contributor.author García-Rupérez, Jaime es_ES
dc.contributor.author Puchades Pla, Rosa es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.date.accessioned 2013-07-08T13:36:04Z
dc.date.issued 2012
dc.identifier.issn 1618-2642
dc.identifier.uri http://hdl.handle.net/10251/30831
dc.description.abstract [EN] Methodology for the functionalization of siliconbased materials employed for the development of photonic label-free nanobiosensors is reported. The studied functionalization based on organosilane chemistry allowed the direct attachment of biomolecules in a single step, maintaining their bioavailability. Using this immobilization approach in probe microarrays, successful specific detection of bacterial DNA is achieved, reaching hybridization sensitivities of 10 pM. The utility of the immobilization approach for the functionalization of label-free nanobiosensors based on photonic crystals and ring resonators was demonstrated using bovine serum albumin (BSA)/anti-BSA as a model system. es_ES
dc.description.sponsorship The authors thank European Union (INTOPSENS FP7-ICT-223932), Ministerio de Ciencia e Innovacion (project no. FEDER CTQ2010-15943) and Generalitat Valenciana (project no. PROMETEO/2010/008 and GV-2010-031) for financial support. en_EN
dc.language Español es_ES
dc.publisher Springer Verlag es_ES
dc.relation MICINN/CTQ2010-15943 es_ES
dc.relation GV/PROMETEO/2010/008 es_ES
dc.relation GV/GV-2010-031
dc.relation.ispartof Analytical and Bioanalytical Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Photonic nanobiosensor es_ES
dc.subject Silicon-based materials es_ES
dc.subject Label free es_ES
dc.subject DNA microarray es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.subject.classification TEORIA DE LA SEÑAL Y COMUNICACIONES es_ES
dc.title Chemical silicon surface modification and bioreceptor attachment to develop competitive integrated photonic biosensors es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1007/s00216-012-6280-4
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/223932 en_EN
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Comunicaciones - Departament de Comunicacions es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Escorihuela Fuentes, J.; Bañuls Polo, MJ.; García Castelló, J.; Toccafondo, V.; García-Rupérez, J.; Puchades Pla, R.; Maquieira Catala, Á. (2012). Chemical silicon surface modification and bioreceptor attachment to develop competitive integrated photonic biosensors. Analytical and Bioanalytical Chemistry. 404:2831-2840. https://doi.org/10.1007/s00216-012-6280-4 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s00216-012-6280-4 es_ES
dc.description.upvformatpinicio 2831 es_ES
dc.description.upvformatpfin 2840 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 404 es_ES
dc.relation.senia 232760
dc.identifier.eissn 1618-2650
dc.identifier.pmid 22872294
dc.contributor.funder European Commission
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Generalitat Valenciana
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