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Production of engineered long-life and male sterile Pelargonium plants

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Production of engineered long-life and male sterile Pelargonium plants

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dc.contributor.author García Sogo, Begoña es_ES
dc.contributor.author Pineda Chaza, Benito José es_ES
dc.contributor.author Roque Mesa, Edelin Marta es_ES
dc.contributor.author Antón Martínez, María Teresa es_ES
dc.contributor.author Atarés Huerta, Alejandro es_ES
dc.contributor.author Borja, Marisé es_ES
dc.contributor.author Beltran Porter, Jose Pio es_ES
dc.contributor.author Moreno Ferrero, Vicente es_ES
dc.contributor.author Cañas Clemente, Luís Antonio es_ES
dc.date.accessioned 2013-09-04T10:15:35Z
dc.date.available 2013-09-04T10:15:35Z
dc.date.issued 2012
dc.identifier.issn 1471-2229
dc.identifier.uri http://hdl.handle.net/10251/31752
dc.description.abstract [EN]Background: Pelargonium is one of the most popular garden plants in the world. Moreover, it has a considerable economic importance in the ornamental plant market. Conventional cross-breeding strategies have generated a range of cultivars with excellent traits. However, gene transfer via Agrobacterium tumefaciens could be a helpful tool to further improve Pelargonium by enabling the introduction of new genes/traits. We report a simple and reliable protocol for the genetic transformation of Pelargonium spp. and the production of engineered long-life and male sterile Pelargonium zonale plants, using the pSAG12::ipt and PsEND1::barnase chimaeric genes respectively. Results: The pSAG12::ipt transgenic plants showed delayed leaf senescence, increased branching and reduced internodal length, as compared to control plants. Leaves and flowers of the pSAG12::ipt plants were reduced in size and displayed a more intense coloration. In the transgenic lines carrying the PsEND1::barnase construct no pollen grains were observed in the modified anther structures, which developed instead of normal anthers. The locules of sterile anthers collapsed 3¿4 days prior to floral anthesis and, in most cases, the undeveloped anther tissues underwent necrosis. Conclusion: The chimaeric construct pSAG12::ipt can be useful in Pelargonium spp. to delay the senescence process and to modify plant architecture. In addition, the use of engineered male sterile plants would be especially useful to produce environmentally friendly transgenic plants carrying new traits by preventing gene flow between the genetically modified ornamentals and related plant species. These characteristics could be of interest, from a commercial point of view, both for pelargonium producers and consumers. es_ES
dc.description.sponsorship This work was funded by grants AGL2009-13388-C03-01 and BIO2009-08134 from the Spanish Ministry of Science and Innovation (MICINN). We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). In the past five years we have received funding from the Spanish Ministry of Science and Innovation (MICINN) and the article-processing charge will be pay with funds from two granted projects. The authors received salaries from two different institutions: The Polytechnic University of Valencia (UPV) or the High Spanish Council of Scientific Research (CSIC). We are not currently applying for a patent related with the content of this manuscript. All the mentioned organisms/institutions do not gain or lose financially from the publication of this manuscript either now or in the future.
dc.language Inglés es_ES
dc.publisher BioMed Central es_ES
dc.relation MICINN/AGL2009-13388-C03-01 es_ES
dc.relation MICINN/BIO2009-08134 es_ES
dc.relation CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI) es_ES
dc.relation Polytechnic University of Valencia (UPV) es_ES
dc.relation High Spanish Council of Scientific Research (CSIC) es_ES
dc.relation.ispartof BMC Plant Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Pelargonium zonale es_ES
dc.subject Pelargonium peltatum es_ES
dc.subject pSAG12 promoter es_ES
dc.subject ipt gene es_ES
dc.subject Engineered long-lived plants es_ES
dc.subject Delayed senescence es_ES
dc.subject Engineered male sterility es_ES
dc.subject PsEND1 promoter es_ES
dc.subject Barnase es_ES
dc.subject Anther ablation es_ES
dc.subject Biosafe ornamentals es_ES
dc.subject.classification GENETICA es_ES
dc.title Production of engineered long-life and male sterile Pelargonium plants es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/1471-2229-12-156
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation García Sogo, B.; Pineda Chaza, BJ.; Roque Mesa, EM.; Antón Martínez, MT.; Atarés Huerta, A.; Borja, M.; Beltran Porter, JP.... (2012). Production of engineered long-life and male sterile Pelargonium plants. BMC Plant Biology. 12:156-171. doi:10.1186/1471-2229-12-156 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1186/1471-2229-12-156 es_ES
dc.description.upvformatpinicio 156 es_ES
dc.description.upvformatpfin 171 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.relation.senia 231284
dc.identifier.pmid 22935247 en_EN
dc.identifier.pmcid PMC3492168 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación


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