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dc.contributor.author | Tamarit López, Jesús![]() |
es_ES |
dc.contributor.author | Morais Ezquerro, Sergi Beñat![]() |
es_ES |
dc.contributor.author | Puchades Pla, Rosa![]() |
es_ES |
dc.contributor.author | Maquieira Catala, Ángel![]() |
es_ES |
dc.date.accessioned | 2013-09-25T11:33:13Z | |
dc.date.issued | 2011-11-02 | |
dc.identifier.issn | 1043-1802 | |
dc.identifier.uri | http://hdl.handle.net/10251/32350 | |
dc.description.abstract | A novel DNA microarrying platform based on oxygen plasma activation of polycarbonate surface of compact disks (DVD) is presented. Carboxylic acid groups are generated in few seconds on polycarbonate in an efficient, fast, and clean way. Following this surface activation strategy, amino-modified oligonucleotide probes were covalently attached, reaching an immobilization density of 2 pmol cm¿2. Atomic force microscopy imaging revealed the nondestructive character of this treatment when applied for short times, allowing for disk scanning in standard DVD drives. DNA assays performed on oxygen plasma treated disks resulted very efficient with maximum hybridization yield of 93% and reaching a low limit of detection (200 pM) for perfect match synthetic oligonucleotide targets when reading the disk with a standard drive as detector. The approach was also evaluated by scoring single nucleotide polymorphisms with a discrimination ratio of 12.8. As proof of concept, the oxygen plasma treated interactive polycarbonate DNA microarraying platform was applied to the detection of PCR products of Salmonella spp., reaching a detection limit of 2 nM that corresponds to a DNA concentration of only 1 c.f.u./mL. The results confirm the suitability of the microarray platform for analysis of biological samples with high sensitivity. | es_ES |
dc.description.sponsorship | This research was funded through projects FEDER MICINN CTQ2010-15943 (CICYT, Spain) and by Generalitat Valenciana (GV/2009/028 and PROMETEO/2010/008). The Spanish Ministerio de Educacion y Ciencia provided J.T.-L. with a grant for his Ph.D. studies. We thank Juan Hurtado for his technical assistance with the plasma reactor. | en_EN |
dc.format.extent | 8 | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation.ispartof | Bioconjugate Chemistry | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Oxygen Plasma Treated Interactive Polycarbonate DNA Microarraying Platform | es_ES |
dc.type | Artículo | es_ES |
dc.embargo.lift | 10000-01-01 | |
dc.embargo.terms | forever | es_ES |
dc.identifier.doi | 10.1021/bc2004268 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//CTQ2010-15943/ES/ESTUDIO DE NUEVAS VIAS DE DESARROLLO DE BIOMEMS PARA SCREENING MASIVO. DEMOSTRACION DE CONCEPTO COMO HERRAMIENTA DE ANALISIS APLICABLE EN "OMICAS"./ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Generalitat Valenciana//GV%2F2009%2F028/ES/Puesta a punto de biosensores en disco compacto/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Generalitat Valenciana//PROMETEO%2F2010%2F008/ES/PROMETEO%2F2010%2F008/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic | es_ES |
dc.description.bibliographicCitation | Tamarit López, J.; Morais Ezquerro, SB.; Puchades Pla, R.; Maquieira Catala, Á. (2011). Oxygen Plasma Treated Interactive Polycarbonate DNA Microarraying Platform. Bioconjugate Chemistry. 22:2573-2580. https://doi.org/10.1021/bc2004268 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http:/dx.doi.org/10.1021/bc2004268 | es_ES |
dc.description.upvformatpinicio | 2573 | es_ES |
dc.description.upvformatpfin | 2580 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 22 | es_ES |
dc.relation.senia | 212790 | |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia | es_ES |