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A Versatile Toolbox for Multiplexed Protein Micropatterning by Laser Lithography

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A Versatile Toolbox for Multiplexed Protein Micropatterning by Laser Lithography

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dc.contributor.author Gropeanu, Mihaela es_ES
dc.contributor.author Bhagawati, Maniraj es_ES
dc.contributor.author Gropeanu, Radu A. es_ES
dc.contributor.author Rodríguez Muñiz, Gemma María es_ES
dc.contributor.author Sundaram, Subramanian es_ES
dc.contributor.author Piehler, Jacob es_ES
dc.contributor.author Campo, Aránzazu del es_ES
dc.date.accessioned 2013-10-31T08:48:59Z
dc.date.issued 2013-03-25
dc.identifier.issn 1613-6810
dc.identifier.uri http://hdl.handle.net/10251/33156
dc.description.abstract Photocleavable oligohistidine peptides (POHP) allow in situ spatial organization of multiple His-tagged proteins onto surfaces functionalized with tris(nitrilotriacetic acid) (tris-NTA). Here, a second generation of POHPs is presented with improved photoresponse and site-specifi c covalent coupling is introduced for generating stable protein assemblies. POHPs with different numbers of histidine residues and a photocleavable linker based on the 4,5-dimethoxy-o-nitrophenyl ethyl chromophore are prepared. These peptides show better photosensitivity than the previously used o-nitrophenyl ethyl derivative. Effi cient and stable caging of tris-NTAfunctionalized surfaces by POHPs comprising 12 histidine residues is demonstrated by multiparameter solid-phase detection techniques. Laser lithographic uncaging by photofragmentation of the POHPs is possible with substantially reduced photodamage as compared to previous approaches. Thus, in situ micropatterning of His-tagged proteins under physiological conditions is demonstrated for the fi rst time. In combination with a short peptide tag for a site-specifi c enzymatic coupling reaction, covalent immobilization of multiple proteins into target micropatterns is possible under physiological conditions. es_ES
dc.format.extent 8 es_ES
dc.language Inglés es_ES
dc.publisher Wiley-VCH Verlag es_ES
dc.relation.ispartof Small es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Protein micropatterns es_ES
dc.subject Laser lithography es_ES
dc.subject Photocleavable peptides es_ES
dc.subject Phototriggers es_ES
dc.subject Histidine tags es_ES
dc.title A Versatile Toolbox for Multiplexed Protein Micropatterning by Laser Lithography es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1002/smll.201201901
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Tecnología Química - Institut Universitari Mixt de Tecnologia Química es_ES
dc.description.bibliographicCitation Gropeanu, M.; Bhagawati, M.; Gropeanu, RA.; Rodríguez Muñiz, GM.; Sundaram, S.; Piehler, J.; Campo, AD. (2013). A Versatile Toolbox for Multiplexed Protein Micropatterning by Laser Lithography. Small. 9(6):838-845. doi:10.1002/smll.201201901 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1002/smll.201201901 es_ES
dc.description.upvformatpinicio 838 es_ES
dc.description.upvformatpfin 845 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 237406
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