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Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.)

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Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.)

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dc.contributor.author Corral Martínez, Patricia es_ES
dc.contributor.author Seguí-Simarro, José M. es_ES
dc.date.accessioned 2013-10-31T13:02:32Z
dc.date.issued 2012
dc.identifier.issn 0014-2336
dc.identifier.uri http://hdl.handle.net/10251/33165
dc.description.abstract Production of doubled haploids (DHs) through androgenesis induction is an important biotechnological tool for plant breeding. In some species, DHs are efficiently obtained through embryogenesis from isolated microspore cultures. In eggplant, however, this process is still at its infancy, despite the economic relevance of this important agricultural crop. To date, only two studies have focused previously on this process, suggesting that in eggplant microspore cultures, the only morphogenic response is callus formation. Given the notable lack of studies on eggplant microspore cultures, in this work we explored this process with different experimental approaches. We studied the response of different cultivars and characterized the development of microspores induced to divide and proliferate. We demonstrated that microspore-derived embryos (MDEs) can be produced in eggplant; however, MDEs stopped at the globular stage, to turn into euploid and principally mixoploid calli. From these calli, 60 % of DH plants could be regenerated. In order to promote microspore induction we evaluated the effect of polyethylene glycol (PEG) and mannitol. PEG, but not mannitol, significantly increased induction of microspore embryogenesis. We also tested the ability of eight different media compositions to promote efficient plant regeneration from calli. In order to test it in a genotype-independent manner, we previously developed a method to generate clonal callus populations derived from single microspore-derived calli. Together, the results presented hereby constitute an efficient way to produce eggplant DHs through microspore culture. In addition, they contribute significant insights into the knowledge of the particularities of androgenesis induction in this species. © 2012 Springer Science+Business Media B.V. es_ES
dc.description.sponsorship We acknowledge Mrs. Nuria Palacios for her excellent technical work, as well as the staff of the COMAV greenhouses for their valuable help. Thanks are also due to Dr. Santiago Vilanova for providing us with the SSRs used in this work. This work was supported by grant from Spanish MICINN AGL2010-17895 to JMSS. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation Spanish MICINN [AGL2010-17895] es_ES
dc.relation.ispartof Euphytica es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Androgenesis es_ES
dc.subject Haploid es_ES
dc.subject In vitro culture es_ES
dc.subject Microspore embryogenesis es_ES
dc.subject Plant regeneration es_ES
dc.subject Solanum melongena es_ES
dc.subject.classification GENETICA es_ES
dc.title Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.) es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1007/s10681-012-0715-z
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Corral Martínez, P.; Seguí-Simarro, JM. (2012). Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.). Euphytica. 187(1):47-61. doi:10.1007/s10681-012-0715-z es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s10681-012-0715-z es_ES
dc.description.upvformatpinicio 47 es_ES
dc.description.upvformatpfin 61 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 187 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 223184
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