Experimental studies on ion-exchange chromatography columns : porosity and capacity

Abstract

Consulta en la Biblioteca ETSI Industriales (8131)

[EN] The ion-exchange chromatography is a technique used to separate the milk whey proteins ß-Lactoglobulin A and B. These proteins are very important to obtain different products for the food industry. From these proteins, amounts of important compounds for the biotechnology industry can be obtained, so it is important to separate them correctly and with the most purity possible. The installation to make this separation is a continuous system made up four ionexchange columns. First of all, it is necessary characterized the columns that will be used. The porosity (¿) and the capacity (¿) of the columns are the two first parameters that should be calculated. To obtain these parameters is not necessary work with the proteins; they are calculated using solutions of salt (Sodium Nitrate) and eluent made up this salt, buffer (Bis Tris Propane) and Hydrochloric acid. The installation used is made up four pumps, columns and the UV Sensor. With this, the concentration of the solutions that are driven into the system is known, and it allow calculate the two desired parameters for each column.