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In vitro evaluation of IgE-mediated hypersensitivity reactions to quinolones

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In vitro evaluation of IgE-mediated hypersensitivity reactions to quinolones

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dc.contributor.author Aranda, A. es_ES
dc.contributor.author Mayorga, C. es_ES
dc.contributor.author Ariza, A. es_ES
dc.contributor.author Doña, I. es_ES
dc.contributor.author Rosado, A. es_ES
dc.contributor.author Blanca-López, N. es_ES
dc.contributor.author Andreu Ros, María Inmaculada es_ES
dc.contributor.author Torres, M.J. es_ES
dc.date.accessioned 2013-11-19T10:39:20Z
dc.date.issued 2011
dc.identifier.issn 0105-4538
dc.identifier.uri http://hdl.handle.net/10251/33726
dc.description.abstract Background: Hypersensitivity IgE-mediated reactions to quinolones are not easy to diagnose, with skin testing inducing false positive results. The aim of the study was to evaluate the in vitro-specific IgE response in patients with immediate allergic reactions to quinolones. Methods: We evaluated 38 patients with confirmed immediate allergic reactions to quinolones. Those with anaphylaxis were considered allergic by clinical history, once other possible causes were ruled out, and those with urticaria by drug provocation. Sepharose-radioimmunoassay (RIA) and basophil activation test (BAT) with ciprofloxacin, moxifloxacin and levofloxacin were performed. Results: The quinolones involved were moxifloxacin (N = 24), ciprofloxacin (N = 11) and levofloxacin (N = 3). Sepharose-RIA was positive in 12 cases (31.57%) and BAT in 27 (71.05%). With Sepharose-RIA, 8 (21%) were positive to ciprofloxacin, 7 (18.4%) to moxifloxacin and 7 (18.4%) to levofloxacin. With BAT, 23 (60.5%) were positive to ciprofloxacin, 12 (31.6%) to moxifloxacin and 8 (21%) to levofloxacin. The specificity of the Sepharose-RIA was demonstrated by inhibition tests. To confirm that the BAT results observed were IgE mediated, the PI3K inhibitor wortmannin was used, with this compound inhibiting the BAT when stimulated with anti-IgE and the different quinolones, but not when fMLP was used as the basophil stimulator. Sepharose-RIA and BAT were repeated in positive cases 1 year later, detecting a decrease in all cases, with four becoming negative. Conclusion: Immediate hypersensitivity reactions to quinolones do occur, with moxifloxacin being the drug most frequently involved. The BAT is a useful method for diagnosing patients. Specific IgE was demonstrated by Sepharose-RIA and inhibition assay. © 2010 John Wiley & Sons A/S. es_ES
dc.description.sponsorship This work has been financed by Grants from Fondo de Investigacion Sanitaria (PS09/01768) and FIS-Thematic Networks and Co-operative Research Centers: RIRAAF (RD07/0064). en_EN
dc.language Inglés es_ES
dc.publisher Wiley-Blackwell es_ES
dc.relation Fondo de Investigacion Sanitaria PS09/01768 es_ES
dc.relation FIS-Thematic Networks and Co-operative Research Centers: RIRAAF RD07/0064 es_ES
dc.relation.ispartof Allergy es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Allergy es_ES
dc.subject Basophil activation test es_ES
dc.subject IgE es_ES
dc.subject Quinolones es_ES
dc.subject Radioimmunoassay es_ES
dc.subject Ciprofloxacin es_ES
dc.subject Dipyrone es_ES
dc.subject Ibuprofen es_ES
dc.subject Immunoglobulin E es_ES
dc.subject Levofloxacin es_ES
dc.subject Moxifloxacin es_ES
dc.subject Omeprazole es_ES
dc.subject Paracetamol es_ES
dc.subject Sepharose es_ES
dc.subject Adult es_ES
dc.subject Aged es_ES
dc.subject Allergic reaction es_ES
dc.subject Anaphylactic shock es_ES
dc.subject Anaphylaxis es_ES
dc.subject Article es_ES
dc.subject Basophil es_ES
dc.subject Basophil degranulation test es_ES
dc.subject Clinical article es_ES
dc.subject Controlled clinical trial es_ES
dc.subject Female es_ES
dc.subject Human es_ES
dc.subject Hypersensitivity reaction es_ES
dc.subject Immune response es_ES
dc.subject In vitro study es_ES
dc.subject Male es_ES
dc.subject Priority journal es_ES
dc.subject Provocation test es_ES
dc.subject Single blind procedure es_ES
dc.subject Urticaria es_ES
dc.subject Adolescent es_ES
dc.subject Anti-Infective Agents es_ES
dc.subject Aza Compounds es_ES
dc.subject Humans es_ES
dc.subject Hypersensitivity, Immediate es_ES
dc.subject Immunologic Tests es_ES
dc.subject Middle Aged es_ES
dc.subject Ofloxacin es_ES
dc.subject Quinolines es_ES
dc.subject Sensitivity and Specificity es_ES
dc.subject Young Adult es_ES
dc.subject.classification QUIMICA ORGANICA es_ES
dc.title In vitro evaluation of IgE-mediated hypersensitivity reactions to quinolones es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1111/j.1398-9995.2010.02460.x
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Tecnología Química - Institut Universitari Mixt de Tecnologia Química es_ES
dc.description.bibliographicCitation Aranda, A.; Mayorga, C.; Ariza, A.; Doña, I.; Rosado, A.; Blanca-López, N.; Andreu Ros, MI.... (2011). In vitro evaluation of IgE-mediated hypersensitivity reactions to quinolones. Allergy. 66:247-254. doi:10.1111/j.1398-9995.2010.02460.x es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://onlinelibrary.wiley.com/doi/10.1111/j.1398-9995.2010.02460.x/pdf es_ES
dc.description.upvformatpinicio 247 es_ES
dc.description.upvformatpfin 254 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 66 es_ES
dc.relation.senia 39790


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