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Development of oligonucleotide microarrays onto si-based surfaces via thioether linkage mediated by UV irradiation

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Development of oligonucleotide microarrays onto si-based surfaces via thioether linkage mediated by UV irradiation

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Escorihuela Fuentes, J.; Bañuls Polo, MJ.; Puchades Pla, R.; Maquieira Catala, Á. (2012). Development of oligonucleotide microarrays onto si-based surfaces via thioether linkage mediated by UV irradiation. Bioconjugate Chemistry. 23(10):2121-2128. doi:10.1021/bc300333a

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/33763

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Title: Development of oligonucleotide microarrays onto si-based surfaces via thioether linkage mediated by UV irradiation
Author:
UPV Unit: Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic
Issued date:
Abstract:
Selective covalent immobilization of thiolated oligonucleotides onto an epoxy-functionalized silicon-substrate can be achieved via light radiation (365 nm). Following this approach, thiol-modified oligonucleotide probes ...[+]
Subjects: Oligonucleotide , Silicon , Sulfide , Article , Controlled study , Cross linking , DNA hybridization , DNA strand , Escherichia coli , Immobilization , Microarray analysis , Nonhuman , Quantum yield , Single nucleotide polymorphism , Ultraviolet irradiation
Copyrigths: Reserva de todos los derechos
Source:
Bioconjugate Chemistry. (issn: 1043-1802 )
DOI: 10.1021/bc300333a
Publisher:
American Chemical Society
Publisher version: http://dx.doi.org/10.1021/bc300333a
Thanks:
Financial support from Ministerio de Ciencia e Innovacion (CTQ2010-15943 BQU) and Generalitat Valenciana (programa PROMETEO/2010/008) is acknowledged. We also thank the NTC of the Universitat Politecnica de Valencia for ...[+]
Type: Artículo

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