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dc.contributor.author | De la Iglesia Jordán, Francisca | es_ES |
dc.contributor.author | Martinez Garcia, Fernando | es_ES |
dc.contributor.author | Hillung, Julia | es_ES |
dc.contributor.author | Cuevas Torrijos, Jose Manuel | es_ES |
dc.contributor.author | Gerrish, Philip J. | es_ES |
dc.contributor.author | Daros Arnau, Jose Antonio | es_ES |
dc.contributor.author | Elena Fito, Santiago Fco | es_ES |
dc.date.accessioned | 2013-12-16T13:25:03Z | |
dc.date.available | 2013-12-16T13:25:03Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0022-538X | |
dc.identifier.uri | http://hdl.handle.net/10251/34543 | |
dc.description.abstract | [EN] A potential drawback of recent antiviral therapies based on the transgenic expression of artificial microRNAs is the ease with which viruses may generate escape mutations. Using a variation of the classic Luria-Delbruck fluctuation assay, we estimated that the spontaneous mutation rate in the artificial microRNA (amiR) target of a plant virus was ca.6 x 10(-5) per replication event. | es_ES |
dc.description.sponsorship | This work was supported by grants BFU2009-06993 from the Spanish Ministerio de Ciencia e Innovación, RGP12/2008 from the Human Frontier Science Program Organization, and PROMETEO2010/019 from Generalitat Valenciana to S.F.E.; by CSIC grant 2010TW0015 to J.-A.D.; and by U.S. National Institutes of Health grants R01GM079843-01 and ARRA PDS#35063 and EC grant FP7231807 to P.J.G. F.M. was supported by a fellowship from Universidad Politénica de Valencia, J.H. was supported by a fellowship from the Spanish Ministerio de Ciencia e Innovación, and J.M.C. was contracted under the CSIC JAE-Doc program. | |
dc.language | Inglés | es_ES |
dc.publisher | American Society for Microbiology | es_ES |
dc.relation.ispartof | Journal of Virology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Plant RNA viruses | es_ES |
dc.subject | Interference | es_ES |
dc.subject | Replication | es_ES |
dc.subject | Resistance | es_ES |
dc.subject | Escape | es_ES |
dc.subject | Identification | es_ES |
dc.subject | Inhibition | es_ES |
dc.title | Luria-Delbrück estimation of Turnip mosaic virus mutation rate in vivo | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1128/JVI.06909-11 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BFU2009-06993/ES/Biologia Evolutiva Y De Sistemas De La Emergencia De Fitovirus De Rna/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/231807/EU/Developing the Framework for an Epidemic Forecast Infrastructure/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/NIH/NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES/5F32GM079843-02/US/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/NIH//R01GM079843-01/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/NIH//ARRA%2FPDS#35063/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/HFSP//RGP0012%2F2008/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F019/ES/Implicaciones evolutivas de la supresión del silenciamiento del RNA por parte de proteína virales/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CSIC//2010TW0015/ES/Evaluation of the durability of artificial microRNA-mediated strategies for plant resistance to RNA viruses/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | De La Iglesia Jordán, F.; Martinez Garcia, F.; Hillung, J.; Cuevas Torrijos, JM.; Gerrish, PJ.; Daros Arnau, JA.; Elena Fito, SF. (2012). Luria-Delbrück estimation of Turnip mosaic virus mutation rate in vivo. Journal of Virology. 86(6):3386-3388. https://doi.org/10.1128/JVI.06909-11 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1128/JVI.06909-11 | es_ES |
dc.description.upvformatpinicio | 3386 | es_ES |
dc.description.upvformatpfin | 3388 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 86 | es_ES |
dc.description.issue | 6 | es_ES |
dc.relation.senia | 232194 | |
dc.identifier.pmid | 22238294 | en_EN |
dc.identifier.pmcid | PMC3302333 | en_EN |
dc.contributor.funder | Ministerio de Ciencia e Innovación | |
dc.contributor.funder | European Commission | |
dc.contributor.funder | Generalitat Valenciana | |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas | |
dc.contributor.funder | National Institutes of Health, EEUU | |
dc.contributor.funder | Universitat Politècnica de València | |
dc.contributor.funder | Human Frontier Science Program Organization |