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Design and development of modular DNA assembly tools for Multigene Engineering and Synthetic Biology in Plants

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Design and development of modular DNA assembly tools for Multigene Engineering and Synthetic Biology in Plants

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dc.contributor.advisor Granell Richart, Antonio es_ES
dc.contributor.advisor Orzáez Calatayud, Diego Vicente es_ES
dc.contributor.author Sarrión Perdigones, Manuel Alejandro es_ES
dc.date.accessioned 2014-02-07T07:17:36Z
dc.date.available 2014-02-07T07:17:36Z
dc.date.created 2014-01-21T11:30:15Z es_ES
dc.date.issued 2014-02-07T07:17:31Z es_ES
dc.identifier.uri http://hdl.handle.net/10251/35399
dc.description.abstract The post-genomics era has put at the disposal of modern plant breeders an endless list of genetic building blocks for the design of new biotechnological crops. After a first wave of single-gene transgenic with controversial public acceptance, genomic information and technology is paving the way for increasingly complex designs based in multiple gene engineering. Those designs aiming at the production of inexpensive health-promoting compounds are most likely to be welcomed by consumers. In this project we plan to develop new multigene assembling tools. During this PhD, a standardized collection of interchangeable genetic parts (including promoters, CDS, P-DNAs, etc) and vectors will be developed. The collection, inspired in Synthetic Biology standards, will be made easy-to-assemble in an interchangeable, semi-idempotent and seamless fashion by the addition of flanking recognition sites of type IIS Restriction endonucleases. The construction of the collection will facilitate multigene engineering and will constitute a first step towards enabling Synthetic Biology in plants. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.rights Reserva de todos los derechos es_ES
dc.source Riunet es_ES
dc.subject Synthetic Biology es_ES
dc.subject Plant Biotechnology es_ES
dc.subject Multigene Engineering es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Design and development of modular DNA assembly tools for Multigene Engineering and Synthetic Biology in Plants
dc.type Tesis doctoral es_ES
dc.identifier.doi 10.4995/Thesis/10251/35399 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Sarrión Perdigones, MA. (2014). Design and development of modular DNA assembly tools for Multigene Engineering and Synthetic Biology in Plants [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/35399 es_ES
dc.description.accrualMethod TESIS es_ES
dc.type.version info:eu-repo/semantics/acceptedVersion es_ES
dc.relation.tesis 4478 es_ES


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