- -

Microbial examination of anaerobic sludge adaptation to animal slurry

RiuNet: Repositorio Institucional de la Universidad Politécnica de Valencia

Compartir/Enviar a

Citas

Estadísticas

  • Estadisticas de Uso

Microbial examination of anaerobic sludge adaptation to animal slurry

Mostrar el registro sencillo del ítem

Ficheros en el ítem

dc.contributor.author Moset Hernández, Verónica es_ES
dc.contributor.author Cerisuelo García, Alba es_ES
dc.contributor.author Ferrer Riera, Pablo es_ES
dc.contributor.author Jiménez Belenguer, Ana Isabel es_ES
dc.contributor.author Bertolini, Edson es_ES
dc.contributor.author Cambra López, María es_ES
dc.date.accessioned 2014-04-09T17:45:55Z
dc.date.issued 2013-03-19
dc.identifier.issn 0959-3330
dc.identifier.uri http://hdl.handle.net/10251/36931
dc.description.abstract The objective of this study was to evaluate changes in the microbial population of anaerobic sludge digesters during the adaptation to pig slurry (PS) using quantitative real-time polymerase chain reaction (qPCR) and qualitative scanning electron microscopy (SEM). Additionally, the relationship between microbial parameters and sludge physicochemical composition and methane yield was examined. Results showed that the addition of PS to an unadapted thermophilic anaerobic digester caused an increase in volatile fatty acids (VFA) concentration, a decrease in removal efficiency and CH4 yield. Additionally, increases in total bacteria and total archaea were observed using qPCR. Scanning electron micrographs provided a general overview of the sludge¿s cell morphology, morphological diversity and degree of organic matter degradation. A change in microbial morphotypes from homogeneous cell morphologies to a higher morphological diversity, similar to that observed in PS, was observed with the addition of PS by SEM. Therefore, the combination of qPCR and SEM allowed expanding the knowledge about the microbial adaptation to animal slurry in thermophilic anaerobic digesters. es_ES
dc.description.sponsorship This work has been supported by the Instituto Nacional de Investigaciones Agrarias of the Ministry of Agriculture of Spain with the projects INIA RTA2006-00143 and INIA RTA2010-0132. en_EN
dc.format.extent 10 es_ES
dc.language Inglés es_ES
dc.publisher Taylor & Francis: STM, Behavioural Science and Public Health Titles es_ES
dc.relation.ispartof Environmental Technology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Anaerobic digestion es_ES
dc.subject Animal slurry es_ES
dc.subject Scanning electron microscopy es_ES
dc.subject Quantitative real-time polymerase chain reaction es_ES
dc.subject Electron Microscopy Service of the UPV es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Microbial examination of anaerobic sludge adaptation to animal slurry es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1080/09593330.2013.848940
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RTA2010-00132-00-00/ES/Estudio de nuevos caracteres para su inclusión en el programa de mejora genética de caprino lechero de raza Murciano Granadina/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEC//RTA2006-00143-00-00/ES/Consolidación del esquema de slelección caprino lechero de raza murciano-granadina en la Comunidad Valenciana con programas de inseminación artificial y de transferencia de embriones/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro Avanzado de Microbiología de Alimentos - Centre Avançat de Microbiologia d'Aliments es_ES
dc.description.bibliographicCitation Moset Hernández, V.; Cerisuelo García, A.; Ferrer Riera, P.; Jiménez Belenguer, AI.; Bertolini, E.; Cambra López, M. (2013). Microbial examination of anaerobic sludge adaptation to animal slurry. Environmental Technology. 35(6):749-758. https://doi.org/10.1080/09593330.2013.848940 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1080/09593330.2013.848940 es_ES
dc.description.upvformatpinicio 749 es_ES
dc.description.upvformatpfin 758 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 35 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 251669
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Ministerio de Educación y Ciencia es_ES
dc.description.references Costa, R. D., Tavares, C. R. G., & Cossich, E. S. (2007). Stabilization of Swine Wastes by Anaerobic Digestion. Environmental Technology, 28(10), 1145-1151. doi:10.1080/09593332808618875 es_ES
dc.description.references Angelidaki, I., Karakashev, D., Batstone, D. J., Plugge, C. M., & Stams, A. J. M. (2011). Biomethanation and Its Potential. Methods in Enzymology, 327-351. doi:10.1016/b978-0-12-385112-3.00016-0 es_ES
dc.description.references Stamatelatou, K., Skiadas, I. V., & Lyberatos, G. (2004). On the behavior of the periodic anaerobic baffled reactor (PABR) during the transition from carbohydrate to protein-based feedings. Bioresource Technology, 92(3), 321-326. doi:10.1016/j.biortech.2003.09.006 es_ES
dc.description.references Snell-Castro, R., Godon, J.-J., Delgenès, J.-P., & Dabert, P. (2005). Characterisation of the microbial diversity in a pig manure storage pit using small subunit rDNA sequence analysis. FEMS Microbiology Ecology, 52(2), 229-242. doi:10.1016/j.femsec.2004.11.016 es_ES
dc.description.references Peu, P., Brugere, H., Pourcher, A.-M., Kerouredan, M., Godon, J.-J., Delgenes, J.-P., & Dabert, P. (2006). Dynamics of a Pig Slurry Microbial Community during Anaerobic Storage and Management. Applied and Environmental Microbiology, 72(5), 3578-3585. doi:10.1128/aem.72.5.3578-3585.2006 es_ES
dc.description.references Liu, F. H., Wang, S. B., Zhang, J. S., Zhang, J., Yan, X., Zhou, H. K., … Zhou, Z. H. (2009). The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis. Journal of Applied Microbiology, 106(3), 952-966. doi:10.1111/j.1365-2672.2008.04064.x es_ES
dc.description.references Hori, T., Haruta, S., Ueno, Y., Ishii, M., & Igarashi, Y. (2006). Dynamic Transition of a Methanogenic Population in Response to the Concentration of Volatile Fatty Acids in a Thermophilic Anaerobic Digester. Applied and Environmental Microbiology, 72(2), 1623-1630. doi:10.1128/aem.72.2.1623-1630.2006 es_ES
dc.description.references Song, L., Laguerre, S., Dumon, C., Bozonnet, S., & O’Donohue, M. J. (2010). A high-throughput screening system for the evaluation of biomass-hydrolyzing glycoside hydrolases. Bioresource Technology, 101(21), 8237-8243. doi:10.1016/j.biortech.2010.05.097 es_ES
dc.description.references Sanz, J. L., & Köchling, T. (2007). Molecular biology techniques used in wastewater treatment: An overview. Process Biochemistry, 42(2), 119-133. doi:10.1016/j.procbio.2006.10.003 es_ES
dc.description.references Miron, Y. (2000). The role of sludge retention time in the hydrolysis and acidification of lipids, carbohydrates and proteins during digestion of primary sludge in CSTR systems. Water Research, 34(5), 1705-1713. doi:10.1016/s0043-1354(99)00280-8 es_ES
dc.description.references De la Rubia, M. A., Perez, M., Romero, L. I., & Sales, D. (2006). Effect of solids retention time (SRT) on pilot scale anaerobic thermophilic sludge digestion. Process Biochemistry, 41(1), 79-86. doi:10.1016/j.procbio.2005.03.073 es_ES
dc.description.references APHA, Standard methods for the examination of water and wastewater. In Greenberg AE, Clesceri LS, Eaton AD, editors. 21th ed. Washington DC, USA: American Public Health Association; 2005. pp. 1325. es_ES
dc.description.references Moset, V., Cerisuelo, A., Sutaryo, S., & Møller, H. B. (2012). Process performance of anaerobic co-digestion of raw and acidified pig slurry. Water Research, 46(16), 5019-5027. doi:10.1016/j.watres.2012.06.032 es_ES
dc.description.references Yu, Y., Kim, J., & Hwang, S. (2006). Use of real-time PCR for group-specific quantification of aceticlastic methanogens in anaerobic processes: Population dynamics and community structures. Biotechnology and Bioengineering, 93(3), 424-433. doi:10.1002/bit.20724 es_ES
dc.description.references Prenafeta-Boldú, F. X., Guivernau, M., Gallastegui, G., Viñas, M., Hoog, G. S., & Elías, A. (2012). Fungal/bacterial interactions during the biodegradation of TEX hydrocarbons (toluene, ethylbenzene and p-xylene) in gas biofilters operated under xerophilic conditions. FEMS Microbiology Ecology, 80(3), 722-734. doi:10.1111/j.1574-6941.2012.01344.x es_ES
dc.description.references Liu, W.-T., Chan, O.-C., & Fang, H. H. P. (2002). Microbial community dynamics during start-up of acidogenic anaerobic reactors. Water Research, 36(13), 3203-3210. doi:10.1016/s0043-1354(02)00022-2 es_ES
dc.description.references Griffin, M. E., McMahon, K. D., Mackie, R. I., & Raskin, L. (1998). Methanogenic population dynamics during start-up of anaerobic digesters treating municipal solid waste and biosolids. Biotechnology and Bioengineering, 57(3), 342-355. doi:10.1002/(sici)1097-0290(19980205)57:3<342::aid-bit11>3.0.co;2-i es_ES
dc.description.references Griffith, G. W., Ozkose, E., Theodorou, M. K., & Davies, D. R. (2009). Diversity of anaerobic fungal populations in cattle revealed by selective enrichment culture using different carbon sources. Fungal Ecology, 2(2), 87-97. doi:10.1016/j.funeco.2009.01.005 es_ES
dc.description.references Shin, S. G., Lee, S., Lee, C., Hwang, K., & Hwang, S. (2010). Qualitative and quantitative assessment of microbial community in batch anaerobic digestion of secondary sludge. Bioresource Technology, 101(24), 9461-9470. doi:10.1016/j.biortech.2010.07.081 es_ES
dc.description.references Molinuevo-Salces, B., González-Fernández, C., Gómez, X., García-González, M. C., & Morán, A. (2012). Vegetable processing wastes addition to improve swine manure anaerobic digestion: Evaluation in terms of methane yield and SEM characterization. Applied Energy, 91(1), 36-42. doi:10.1016/j.apenergy.2011.09.010 es_ES


Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem