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Bisphenol A determination in baby bottles by chemiluminescent enzyme-linked immunosorbent assay, lateral flow immunoassay and liquid chromatography tandem mass spectrometry

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Bisphenol A determination in baby bottles by chemiluminescent enzyme-linked immunosorbent assay, lateral flow immunoassay and liquid chromatography tandem mass spectrometry

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dc.contributor.author Maiolini, Elisabetta es_ES
dc.contributor.author Ferri, Elida es_ES
dc.contributor.author Pitasi, Agata Laura es_ES
dc.contributor.author Montoya Baides, Ángel es_ES
dc.contributor.author Di Giovanni, Manuela es_ES
dc.contributor.author Errani, Ermanno es_ES
dc.contributor.author Girotti, Stefano es_ES
dc.date.accessioned 2014-05-26T09:17:32Z
dc.date.issued 2014
dc.identifier.issn 0003-2654
dc.identifier.uri http://hdl.handle.net/10251/37750
dc.description.abstract Two immunoassays, a Lateral Flow ImmunoAssay (LFIA) based on colloidal gold nanoparticle labels and an indirect competitive chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA), were developed and a high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was optimized to assess the possible release of bisphenol A (BPA, 4,4¿-isopropylidenediphenol) from different plastic baby bottles treated with simulating solutions. Coating conjugate concentration, anti-BPA antibody dilution, incubation time of the primary and secondary antibodies, and tolerance to different organic solvents were optimized to obtain the best performance of the ELISA with chemiluminescent end-point detection. The influence of different buffers on LFIA performance was also evaluated. Both methods showed good repeatability (mean CV value around 13%) and sensitivity. Reproducibility tests for CL-ELISA gave a mean CV value of about 25%. The IC50 and Limit of Detection (LOD) values of CL-ELISA were 0.2 and 0.02 ng mL¿1, respectively. The LOD of LFIA was 0.1 ¿g mL¿1. A LC-MS/MS method was also optimized. The separation was performed in a C18 column with a triple-quadrupole mass spectrometer with electrospray ionisation interface. The method showed a good linearity in the range 2 to 500 ng mL¿1, with a regression coefficient of 0.998. In the simulating solutions the detection and quantification limits, calculated by the signal to noise level of 3 (S/N = 3), were 5.8 ng mL¿1 and 17.4 ng mL¿1, respectively. This limit of quantification was about 3 and 35 times lower than the permitted limits set by the official method CEN/TS 13130-13 (0.05 ¿g mL¿1) and by the Directive 2004/19/EC (0.6 ¿g mL¿1), respectively. The methods were applied to determine BPA release from baby bottles, performing repeated procedures according to EU and national regulations. The results demonstrated that no BPA migration from the tested plastic materials occurred with only one exception. The migrated amount, above the regulatory limits, was detected by all the mentioned assays es_ES
dc.description.sponsorship This work was supported by a grant from the University of Bologna (RFO-Focused Fundamental Research Projects 2000-2011) grants. en_EN
dc.language Inglés es_ES
dc.publisher Royal Society of Chemistry es_ES
dc.relation.ispartof Analyst es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Bisphenol A determination in baby bottles by chemiluminescent enzyme-linked immunosorbent assay, lateral flow immunoassay and liquid chromatography tandem mass spectrometry es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1039/c3an00552f
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano - Institut Interuniversitari d'Investigació en Bioenginyeria i Tecnologia Orientada a l'Ésser Humà es_ES
dc.description.bibliographicCitation Maiolini, E.; Ferri, E.; Pitasi, AL.; Montoya Baides, Á.; Di Giovanni, M.; Errani, E.; Girotti, S. (2014). Bisphenol A determination in baby bottles by chemiluminescent enzyme-linked immunosorbent assay, lateral flow immunoassay and liquid chromatography tandem mass spectrometry. Analyst. 139(1):318-324. doi:10.1039/c3an00552f es_ES
dc.description.accrualMethod S es_ES
dc.description.upvformatpinicio 318 es_ES
dc.description.upvformatpfin 324 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 139 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 263998
dc.contributor.funder Università di Bologna es_ES
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