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Dispersive solid-phase extraction and immunoassay with internal reference calibration using fatty acid-coated inorganic fluorescent nanoparticles

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Dispersive solid-phase extraction and immunoassay with internal reference calibration using fatty acid-coated inorganic fluorescent nanoparticles

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dc.contributor.author Cháfer Pericás, María Consuelo es_ES
dc.contributor.author Balaguer, Ángel es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.contributor.author Puchades Pla, Rosa es_ES
dc.date.accessioned 2014-07-17T10:13:52Z
dc.date.issued 2013
dc.identifier.issn 0003-2697
dc.identifier.uri http://hdl.handle.net/10251/38870
dc.description.abstract Dispersive solid-phase extraction (dSPE) using fatty acid-coated Eu2O3 nanoparticles (NPs) was developed, and a direct immunoassay was carried out employing these NPs as support. Secondary antibodies labeled with fluorophore groups were used as reporters, and the intrinsic optical properties of the Eu2O3 NPs were employed as an internal calibration of the detection system. The methodology was optimized for both dSPE¿NP amount, sample volume, extraction time, ionic strength, and pH¿and immunoassay¿immunoreagent concentrations, ionic strength, and incubation time. As proof of concept, the methodology was applied to the bovine serum albumin (BSA)/anti-BSA system, and precision of the method was between 5% and 17% with an IC50 of 100 nM. Then, water samples with high saline content (sea water) were assayed to observe the matrix effect, and average recoveries (n = 3) between 78% and 108% were obtained, demonstrating the reliability of the developed analytical method. Finally, the simultaneous dSPE¿immunoassay methodology was applied to other compounds with very different chemical characteristics such as an oligonucleotide, the antibiotic sulfamerazine, and the pesticide chlorpyriphos. Although the IC50 values for sulfamerazine were approximately 2400 nM, satisfactory standard curves were obtained. However, poor reproducibility and sensitivity results were obtained for the oligonucleotide and chlorpyriphos preliminary assays. es_ES
dc.description.sponsorship The authors acknowledge the Generalitat Valenciana (Project Prometeo 2010, 008) for the financial support received. C.C.-P. gratefully thanks the Spanish Ministerio de Educacion y Ciencia for a postdoctoral contract with financing from the European Social Fund. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier Masson es_ES
dc.relation.ispartof Analytical Biochemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Nanoparticle es_ES
dc.subject Immunoassay es_ES
dc.subject Dispersive SPE es_ES
dc.subject Fluorescence es_ES
dc.subject Internal reference es_ES
dc.subject BSA es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Dispersive solid-phase extraction and immunoassay with internal reference calibration using fatty acid-coated inorganic fluorescent nanoparticles es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1016/j.ab.2012.09.019
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F008/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Cháfer Pericás, MC.; Balaguer, Á.; Maquieira Catala, Á.; Puchades Pla, R. (2013). Dispersive solid-phase extraction and immunoassay with internal reference calibration using fatty acid-coated inorganic fluorescent nanoparticles. Analytical Biochemistry. 432(1):31-37. https://doi.org/10.1016/j.ab.2012.09.019 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.ab.2012.09.019 es_ES
dc.description.upvformatpinicio 31 es_ES
dc.description.upvformatpfin 37 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 432 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 232763
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder Ministerio de Educación y Ciencia es_ES


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