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Parthenogenic blastocysts cultured under in vivo conditions exhibit proliferation and differentiation expression genes similar to those of normal embryos.

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Parthenogenic blastocysts cultured under in vivo conditions exhibit proliferation and differentiation expression genes similar to those of normal embryos.

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dc.contributor.author Naturil Alfonso, Carmen es_ES
dc.contributor.author Saenz de Juano Ribes, María de los Desamparados es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.contributor.author Marco Jiménez, Francisco es_ES
dc.date.accessioned 2014-07-17T10:29:56Z
dc.date.issued 2011
dc.identifier.issn 0378-4320
dc.identifier.uri http://hdl.handle.net/10251/38877
dc.description.abstract Parthenote embryos offer multiple possibilities in biotechnological investigation, such as stem cell research. However, there is still a dearth of knowledge of this kind of embryo. In this study, development and ploidy were analysed in parthenotes under in vitro and in vivo culture conditions. Subsequently, using real-time PCR, the expressions of factor OCT-4, Vascular Endothelial Growth Factor, Epidermal Growth Factor Receptor 3 and Transforming Growth Factor ß2 genes were analysed to compare the embryo types at the blastocyst stage. Development and implantation of parthenote embryos were described after transfer at day 10 of pregnancy. Parthenotes showed similar blastocyst development for both culture conditions and most of the parthenotes produced were diploid. However, parthenotes developed under in vivo conditions showed similar mRNA expression of OCT-4, VEGF and TGF-ß2 to 5 and 6 day old blastocysts. In contrast, parthenotes developed under in vitro conditions had altered the expression pattern of these genes, except for erbB3 mRNA. Finally, transferred parthenotes had the ability to implant but showed severe growth retardation and lesser size. This is the first demonstration of the influence of culture conditions on parthenote mRNA expression. Our study highlights the importance of culture conditions in subsequent uses of parthenotes, such as the production of stem cell lines. © 2011 Elsevier B.V. es_ES
dc.description.sponsorship This work was supported by Generalitat Valenciana research program (Prometeo 2009-8260: 125) and by the Spanish Research Projects (CICYT AGL2008-03274). The authors thank Neil Macowan Language Services for revising the English version of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier Masson es_ES
dc.relation Generalitat Valenciana research program [Prometeo 2009-8260: 125] es_ES
dc.relation Spanish Research Projects [CICYT AGL2008-03274] es_ES
dc.relation.ispartof Animal Reproduction Science es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Culture conditions es_ES
dc.subject In vitro es_ES
dc.subject In vivo es_ES
dc.subject MRNA expression es_ES
dc.subject Animal es_ES
dc.subject Animal embryo es_ES
dc.subject Article es_ES
dc.subject Blastocyst es_ES
dc.subject Cell culture es_ES
dc.subject Cell differentiation es_ES
dc.subject Cell proliferation es_ES
dc.subject Comparative study es_ES
dc.subject Cytology es_ES
dc.subject Embryo culture es_ES
dc.subject Embryo development es_ES
dc.subject Evaluation es_ES
dc.subject Female es_ES
dc.subject Gene expression es_ES
dc.subject Gene expression profiling es_ES
dc.subject Genetics es_ES
dc.subject Metabolism es_ES
dc.subject Nidation es_ES
dc.subject Parthenogenesis es_ES
dc.subject Physiology es_ES
dc.subject Rabbit es_ES
dc.subject Animals es_ES
dc.subject Cells, Cultured es_ES
dc.subject Embryo Culture Techniques es_ES
dc.subject Embryo Implantation es_ES
dc.subject Embryo, Mammalian es_ES
dc.subject Embryonic Development es_ES
dc.subject Rabbits es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Parthenogenic blastocysts cultured under in vivo conditions exhibit proliferation and differentiation expression genes similar to those of normal embryos. es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1016/j.anireprosci.2011.08.005
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Naturil Alfonso, C.; Saenz De Juano Ribes, MDLD.; Peñaranda, D.; Vicente Antón, JS.; Marco Jiménez, F. (2011). Parthenogenic blastocysts cultured under in vivo conditions exhibit proliferation and differentiation expression genes similar to those of normal embryos. Animal Reproduction Science. (127):222-228. doi:10.1016/j.anireprosci.2011.08.005 es_ES
dc.description.accrualMethod Senia es_ES
dc.description.upvformatpinicio 222 es_ES
dc.description.upvformatpfin 228 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.issue 127 es_ES
dc.relation.senia 198234


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