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Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions

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Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions

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Nombre: Naturil;Saenz;Peñ ...
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dc.contributor.author Naturil Alfonso, Carmen es_ES
dc.contributor.author Saenz de Juano Ribes, María de los Desamparados es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.contributor.author Marco Jiménez, Francisco es_ES
dc.date.accessioned 2014-08-28T09:57:45Z
dc.date.available 2014-08-28T09:57:45Z
dc.date.issued 2012-12
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10251/39269
dc.description.abstract Parthenogenetic embryos are one attractive alternative as a source of embryonic stem cells, although many aspects related to the biology of parthenogenetic embryos and parthenogenetically derived cell lines still need to be elucidated. The present work was conducted to investigate the gene expression profile of rabbit parthenote embryos cultured under in vivo conditions using microarray analysis. Transcriptomic profiles indicate 2541 differentially expressed genes between parthenotes and normal in vivo fertilised blastocysts, of which 76 genes were upregulated and 16 genes downregulated in in vivo cultured parthenote blastocyst, using 3 fold-changes as a cut-off. While differentially upregulated expressed genes are related to transport and protein metabolic process, downregulated expressed genes are related to DNA and RNA binding. Using microarray data, 6 imprinted genes were identified as conserved among rabbits, humans and mice: GRB10, ATP10A, ZNF215, NDN, IMPACT and SFMBT2. We also found that 26 putative genes have at least one member of that gene family imprinted in other species. These data strengthen the view that a large fraction of genes is differentially expressed between parthenogenetic and normal embryos cultured under the same conditions and offer a new approach to the identification of imprinted genes in rabbit. © 2012 Naturil-Alfonso et al. es_ES
dc.description.sponsorship This work was supported by Generalitat Valenciana research programme (Prometeo 2009/125). Carmen Naturil was supported by Generalitat Valenciana research programme (Prometeo 2009/125). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS ONE es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Animal cell es_ES
dc.subject Article es_ES
dc.subject ATP10A gene es_ES
dc.subject Blastocyst es_ES
dc.subject DNA binding es_ES
dc.subject Down regulation es_ES
dc.subject Embryo es_ES
dc.subject Embryo culture es_ES
dc.subject Embryo development es_ES
dc.subject Fertilization es_ES
dc.subject Gene es_ES
dc.subject Gene expression es_ES
dc.subject Gene expression profiling es_ES
dc.subject Gene identification es_ES
dc.subject Genetic association es_ES
dc.subject Genetic conservation es_ES
dc.subject Genetic variability es_ES
dc.subject Genome imprinting es_ES
dc.subject GRB10 gene es_ES
dc.subject IMPACT gene es_ES
dc.subject In vivo study es_ES
dc.subject Microarray analysis es_ES
dc.subject NDN gene es_ES
dc.subject Nonhuman es_ES
dc.subject Nucleotide sequence es_ES
dc.subject Parthenogenetic blastocyst es_ES
dc.subject Protein metabolism es_ES
dc.subject Protein transport es_ES
dc.subject Rabbit es_ES
dc.subject RNA binding es_ES
dc.subject Sfmbt2 gene es_ES
dc.subject Transcriptomics es_ES
dc.subject Upregulation es_ES
dc.subject ZNF215 gene es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.pone.0051271
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2009%2F125/ES/Efecto de la crioconservación de embriones sobre el desarrollo y el re-establecimiento de poblaciones/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Naturil Alfonso, C.; Saenz De Juano Ribes, MDLD.; Peñaranda, D.; Vicente Antón, JS.; Marco Jiménez, F. (2012). Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions. PLoS ONE. 7(12):1-11. https://doi.org/10.1371/journal.pone.0051271 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1371/journal.pone.0051271 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 11 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 12 es_ES
dc.relation.senia 229338
dc.identifier.pmid 23251477 en_EN
dc.identifier.pmcid PMC3522381 en_EN
dc.contributor.funder Generalitat Valenciana es_ES
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