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Live birth from slow-frozen rabbit oocytes after in vivo fertilisation

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Live birth from slow-frozen rabbit oocytes after in vivo fertilisation

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dc.contributor.author Jiménez Trigos, María Estrella es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.contributor.author Marco Jiménez, Francisco es_ES
dc.date.accessioned 2014-08-28T12:15:14Z
dc.date.available 2014-08-28T12:15:14Z
dc.date.issued 2013-12
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10251/39277
dc.description.abstract In vivo fertilisation techniques such as intraoviductal oocyte transfer have been considered as alternatives to bypass the inadequacy of conventional in vitro fertilisation in rabbit. There is only one study in the literature, published in 1989, that reports live offspring from cryopreserved rabbit oocytes. The aim of the present study was to establish the in vivo fertilisation procedure to generate live offspring with frozen oocytes. First, the effect of two recipient models (i) ovariectomised or (ii) oviduct ligated immediately after transfer on the ability of fresh oocytes to fertilise were compared. Second, generation of live offspring from slow-frozen oocytes was carried out using the ligated oviduct recipient model. Throughout the experiment, recipients were artificially inseminated 9 hours prior to oocyte transfer. In the first experiment, two days after unilateral transfer of fresh oocytes, oviducts and uterine horns were flushed to assess embryo recovery rates. The embryo recovery rates were low compared to control in both ovariectomised and ligated oviduct groups. However, ligated oviduct recipient showed significantly (P<0.05) higher embryo recovery rates compared to ovariectomised and control-transferred. In the second experiment, using bilateral oviduct ligation model, all females that received slow-frozen oocytes became pregnant and delivered a total of 4 live young naturally. Thus, in vivo fertilisation is an effective technique to generate live offspring using slow-frozen oocytes in rabbits. es_ES
dc.description.sponsorship This work was supported by the Spanish Research Project AGL2011-30170-C02-01 (CICYT) and by funds from the Generalitat Valenciana Research Programme (Prometeo 2009/125). Estrella Jimenez was supported by a research grant from the Education Ministry of the Valencian Regional Government (programme VALi+d (ACIF/2010/262). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS ONE es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Intracytoplasmic sperm injection es_ES
dc.subject Messenger-RNA expression es_ES
dc.subject Different culture-systems es_ES
dc.subject Thawed bovine oocytes es_ES
dc.subject Embryo cryopreservation es_ES
dc.subject Mouse oocytes es_ES
dc.subject Vitro es_ES
dc.subject Survival es_ES
dc.subject Vitrification es_ES
dc.subject Oviduct es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Live birth from slow-frozen rabbit oocytes after in vivo fertilisation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.pone.0083399
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2011-30170-C02-02/ES/ESTUDIO DEL SISTEMA INMUNE DE DIFERENTES LINEAS GENETICAS CUNICOLAS Y SU RESPUESTA FRENTE A DESAFIOS PRODUCTIVOS E INFECCIOSOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2009%2F125/ES/Efecto de la crioconservación de embriones sobre el desarrollo y el re-establecimiento de poblaciones/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACIF%2F2010%2F262/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Jiménez Trigos, ME.; Vicente Antón, JS.; Marco Jiménez, F. (2013). Live birth from slow-frozen rabbit oocytes after in vivo fertilisation. PLoS ONE. 8(17):1-8. https://doi.org/10.1371/journal.pone.0083399 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1371/journal.pone.0083399 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 8 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 17 es_ES
dc.relation.senia 253735
dc.identifier.pmid 24358281 en_EN
dc.identifier.pmcid PMC3866232 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Generalitat Valenciana es_ES
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